Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles

Afaq, Farrukh; Abidi, Parveen; Matin, R.; Rahman, Qamar

doi:10.1016/s0378-4274(98)00151-9

Cited by 24 publications

(10 citation statements)

References 32 publications

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“…The cytokine/chemokine data illustrate that nanoscale TiO 2 of different structures and associated surface chemistries induce an inflammatory response in the PTE construct. These data are corroborated by previous studies reporting ultrafine TiO 2 particles cause an increase in tissue inflammation and alter macrophage chemotactic response 31. Together, this data concludes that at a dose of 1.56 μM, TiO 2 nanoparticles are reactive enough to initiate an inflammatory response.…”

Section: Resultssupporting

confidence: 88%

Exposure to Titanium Dioxide Nanomaterials Provokes Inflammation of an in Vitro Human Immune Construct

Schanen¹,

et al. 2009

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Nanoparticle technology is undergoing significant expansion largely because of the potential of nanoparticles as biomaterials, drug delivery vehicles, cancer therapeutics, and immunopotentiators. Incorporation of nanoparticle technologies for in vivo applications increases the urgency to characterize nanomaterial immunogenicity. This study explores titanium dioxide, one of the most widely manufactured nanomaterials, synthesized into its three most common nanoarchitectures: anatase (7-10 nm), rutile (15-20 nm), and nanotube (10-15 nm diameters, 70-150 nm length). The fully human autologous MIMIC immunological construct has been utilized as a predictive, nonanimal alternative to diagnose nanoparticle immunogenicity. Cumulatively, treatment with titanium dioxide nanoparticles in the MIMIC system led to elevated levels of proinflammatory cytokines and increased maturation and expression of costimulatory molecules on dendritic cells. Additionally, these treatments effectively primed activation and proliferation of naïve CD4 + T cells in comparison to dendritic cells treated with micrometer-sized (>1 μm) titanium dioxide, characteristic of an in vivo inflammatory response.

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Section: Resultssupporting

confidence: 88%

Exposure to Titanium Dioxide Nanomaterials Provokes Inflammation of an in Vitro Human Immune Construct

Schanen¹,

et al. 2009

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“…Antioxidant enzymes play a main role in the defence of mammalian blood, and these enzymes can be induced or inhibited in the blood cells exposed to different toxicants (Afaq et al, 1998;Prasad et al, 2006). In this study, there is considerable evidence that boron compounds (boric acid, borax, ulexite and colemanite) influence the antioxidant enzyme activities as related to the dose not leading to any genetic damage in human blood.…”

Section: Discussionmentioning

confidence: 94%

Effects of Some Boron Compounds on Peripheral Human Blood

Türkez

Geyikoğlu

Tatar

et al. 2007

Zeitschrift Für Naturforschung C

121

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Peripheral blood cultures were exposed to various doses (5 to 500 mg/L) of boron compounds. Sister-chromatid exchange, micronucleus and chromosomal aberration tests were applied to estimate the DNA damage, and biochemical parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glucose-6-phosphate dehydrogenase, total glutathione, malondialdehyde and total antioxidant capacity) were examined to determine oxidative stress. According to our findings, various boron compounds at low doses were useful in supporting antioxidant enzyme activities in human blood cultures. It was found that the boron compounds do not have genotoxic effects even in the highest concentrations, though in increasing doses they constitute oxidative stress. It is concluded that the tested boron compounds can be used safely, but it is necessary to consider the tissue damages which are likely to appear depending on the oxidative stress.

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“…Recent studies have revealed that exposure to nano-TiO 2 causes inflammation, pulmonary damage, fibrosis, and lung tumors [Yamadori et al, 1986;Driscoll et al, 1991;Oberdorster et al, 1992;Baggs et al, 1997;Afaq et al, 1998].…”

Section: Discussionmentioning

confidence: 99%

Titanium dioxide nanoparticles trigger p53‐mediated damage response in peripheral blood lymphocytes

Kang

Kim

Lee

et al. 2008

Environ and Mol Mutagen

327

156

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Titanium dioxide nanoparticles (nano-TiO2) are widely used as a photocatalyst in air and water remediation. These nanoparticles are known to induce toxicity; however, their cytotoxic mechanism is not fully understood. In this study, we investigated the underlying mechanism of nano-TiO2-induced cytotoxicity in peripheral blood lymphocytes. We examined the genotoxic effects of nano-TiO2 in lymphocytes using alkaline single-cell gel electrophoresis (Comet) and cytokinesis-block micronucleus (CBMN) assays. Lymphocytes treated with nano-TiO2 showed significantly increased micronucleus formation and DNA breakage. Western-blot analysis to identify proteins involved in the p53-mediated response to DNA damage revealed the accumulation of p53 and activation of DNA damage checkpoint kinases in nano-TiO2-treated lymphocytes. However, p21 and bax, downstream targets of p53, were not affected, indicating that nano-TiO2 does not stimulate transactivational activity of p53. The generation of reactive oxygen species (ROS) in nano-TiO2-treated cells was also observed, andN-acetylcysteine (NAC) supplementation inhibited the level of nano-TiO2-induced DNA damage. Given that ROS-induced DNA damage leads to p53 activation in the DNA damage response, our results suggest that nano-TiO2 induces ROS generation in lymphocytes, thereby activating p53-mediated DNA damage checkpoint signals.

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Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles

Cited by 24 publications

References 32 publications

Exposure to Titanium Dioxide Nanomaterials Provokes Inflammation of an in Vitro Human Immune Construct

Exposure to Titanium Dioxide Nanomaterials Provokes Inflammation of an in Vitro Human Immune Construct

Effects of Some Boron Compounds on Peripheral Human Blood

Titanium dioxide nanoparticles trigger p53‐mediated damage response in peripheral blood lymphocytes

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