Activation of Akt and JNK/Nrf2/NQO1 pathway contributes to the protective effect of coptisine against AAPH-induced oxidative stress

Hu, Yinran; Ma, Hang; Zou, Zongyao; He, Kai; Xiao, Yali; Wang, Yue; Feng, Min; Ye, Xiaoli; Li, Xuegang

doi:10.1016/j.biopha.2016.11.031

Cited by 53 publications

(33 citation statements)

References 42 publications

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“…Then, zebrafish embryos were rinsed using fresh embryo media until 24 hpf were completed. The assay was performed in triplicate (Hu et al., 2017; Vilcacundo et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

Argentine Patagonia barberry chemical composition and evaluation of its antioxidant capacity

et al. 2020

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An important portion of vitamins, minerals and polyphenols components in human diet are captured from fruit consumption. Argentinean Patagonia Berberis microphylla was characterized with the phenolic content, the proximate composition and the identification and quantification of anthocyanins, not‐anthocyanins and proteins. The antioxidant capacity of berberis ethanolic extracts (EB) was determined by the 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assays. EB was used to reduce production of reactive substances species (ROS) in zebrafish. EB presented a total polyphenols content of 1,035.03 mg GAE/100 g fresh weight (FW). EB presented an ABTS value of 116.25 ± 17 μmol TE/g FW. EB presented a DPPH value of 137.80 ± 1.90 μmol TE/g FW. EB was able of reducing the ROS in zebrafish. Berberies Protein Isolate (BPI) presented proteins with bands from 15 to 62 kDa. BPI presented an ABTS value of 593.11 ± 8.60 μmol TE/g. The BPI duodenal digest presented a value of 641.07 ± 12.60 μmol TE/g digests. Practical applications The practical applications of the present study are to increase scientific knowledge for consumers about the quality and benefits of the consumption of the native fruit (Berberis microphylla) from the Patagonia region of Argentine. This work describes the protein profile of berberies, their digestibility and their antioxidant activity. This study allows to better understand the phytonutrients that make up this fruit. Future studies may identify the peptides present in hydrolyzates. The bio‐compounds of this fruit could be used as functional ingredients by the food industry for different purposes.

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“…Then, zebrafish embryos were rinsed using fresh embryo media until 24 hpf were completed. The assay was performed in triplicate (Hu et al., 2017; Vilcacundo et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

Argentine Patagonia barberry chemical composition and evaluation of its antioxidant capacity

et al. 2020

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“…In addition, coptisine has been shown to improve cardiac systolic and diastolic dysfunction, and to ameliorate mitochondrial respiratory dysfunction by high doses of isoproterenol, a synthetic catecholamine and β-adrenergic agonist, due to its strong antioxidant activity (Gong et al 2012). Moreover, recent studies have shown that coptisine acts as a powerful ROS scavenger through activating the nuclear factorerythroid-2-related factor 2 (Nrf2) signaling pathway, and can effectively exacerbate oxidative stress (Hu et al 2017;Luo et al 2018). These antioxidant defense effects of Nrf2 signal transduction by coptisine seem to play an important role in regulating cell proliferation and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Activation of the Nrf2/HO-1 signaling pathway contributes to the protective effects of coptisine against oxidative stress-induced DNA damage and apoptosis in HaCaT keratinocytes

Choi

2019

gpb

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In this study, the protective effect of coptisine on the oxidative damage-mediated apoptosis was evaluated in cultured human HaCaT keratinocytes. The results demonstrate that preincubation of cells with coptisine prior to H 2 O 2 stimulation resulted in significant inhibition of cytotoxicity and DNA damage associated with the inhibition of reactive oxygen species (ROS) accumulation. Coptisine also restored H 2 O 2-induced mitochondrial dysfunction and decrease of ATP production, and prevented apoptosis by inhibiting Bax/Bcl-2 ratio, caspase-3 activity, and poly(ADP-ribose) polymerase degradation. Interestingly, the expressions of nuclear factor-erythroid-2-related factor 2 (Nrf2) and its active form, phosphorylated Nrf2, were strikingly promoted by coptisine in the presence of H 2 O 2 , which was associated with a marked increase in the expression of heme oxygenase-1 (HO-1). However, coptisine-induced HO-1 expression was completely abrogated by Nrf2-specific small interfering RNA (Nrf2-siRNA), which suggests that the increased expression of HO-1 by coptisine is Nrf2-dependent. In addition, Nrf2-siRNA transfection significantly eliminated the protective effect of coptisine on H 2 O 2-induced cytotoxicity, and this effect was similar to that by zinc protoporphyrin IX (ZnPP), an HO-1 specific inhibitor. Furthermore, the protective effects of coptisine against H 2 O 2-induced cytotoxicity were abolished by ZnPP, indicating that coptisine protects keratinocytes against oxidative stress-induced injury through activation of the Nrf2/HO-1 signaling pathway.

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“…Interestingly, the effect of coptisine (0.0025‐0.01 mg/mL, 12 hpf; 0.06‐0.25 μmol/L, 3 hours) on MAPK subfamilies ERK, JNK and p38 demonstrated to be reversed in oxidative injury model of AAPH‐challenged adult zebrafish . It protected zebrafish against oxidative injury through up‐regulating mRNA expression of ERK, JNK, p38, NQO1, Nrf2 and Akt, and this effect was further confirmed in anther in vitro model, AAPH‐stimulated HepG2 cells.…”

Section: Other Propertiesmentioning

confidence: 73%

Coptisine fromCoptis chinensisexerts diverse beneficial properties: A concise review

Luo

Deng

et al. 2019

J Cellular Molecular Medi

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Coptisine is a natural small‐molecular compound extracted from Coptis chinensis (CC) with a history of using for thousands of years. This work aimed at summarizing coptisine's activity and providing advice for its clinical use. We analysed the online papers in the database of SciFinder, Web of Science, PubMed, Google scholar and CNKI by setting keywords as ‘coptisine’ in combination of ‘each pivotal pathway target’. Based on the existing literatures, we find (a) coptisine exerted potential to be an anti‐cancer, anti‐inflammatory, CAD ameliorating or anti‐bacterial drug through regulating the signalling transduction of pathways such as NF‐κB, MAPK, PI3K/Akt, NLRP3 inflammasome, RANKL/RANK and Beclin 1/Sirt1. However, we also (b) observe that the plasma concentration of coptisine demonstrates obvious non‐liner relationship with dosage, and even the highest dosage used in animal study actually cannot reach the minimum concentration level used in cell experiments owing to the poor absorption and low availability of coptisine. We conclude (a) further investigations can focus on coptisine's effect on caspase‐1‐involved inflammasome assembling and pyroptosis activation, as well as autophagy. (b) Under circumstance of promoting coptisine availability by pursuing nano‐ or microrods strategies or applying salt‐forming process to coptisine, can it be introduced to clinical trial.

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Activation of Akt and JNK/Nrf2/NQO1 pathway contributes to the protective effect of coptisine against AAPH-induced oxidative stress

Cited by 53 publications

References 42 publications

Argentine Patagonia barberry chemical composition and evaluation of its antioxidant capacity

Argentine Patagonia barberry chemical composition and evaluation of its antioxidant capacity

Activation of the Nrf2/HO-1 signaling pathway contributes to the protective effects of coptisine against oxidative stress-induced DNA damage and apoptosis in HaCaT keratinocytes

Coptisine fromCoptis chinensisexerts diverse beneficial properties: A concise review

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