Activation of adipose tissue glycerokinase contributes to increased white adipose tissue mass in mice fed a high-fat diet

Buzelle, Samyra Lopes; Przygodda, Franciele; Rossi-Valentim, Rafael; Ferreira, Graziella Nascimento; Garófalo, Maria Antonieta Rissato; Alves, Vâni Maria; Chaves, Valéria Ernestânia; Navegantes, Luiz Carlos Carvalho; Kettelhut, Ísis C.

doi:10.1007/s12020-020-02288-3

Cited by 5 publications

(4 citation statements)

References 53 publications

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“…It has been demonstrated that prolonged high-protein diet decreased the lipid accumulation by depression of lipogenic genes expression in the liver in black seabream ( Ancherythroculter nigrocauda ) ( 40 ), which seems to be similar in skeletal muscle in this study, as certain DEGs on lipid synthesis were screen out between different dietary protein groups. In the white muscle of fish subjected to a high-protein diet, both the expression of glycerol kinase (GyK), an important enzyme for producing glycerol 3-phosphate in lipogenesis ( 41 ), and the mRNA transcription of nocturnin (NOC), a circadian-regulated protein involved in adipogenesis through cooperation with PPAR-γ ( 42 ), were downregulated. Furthermore, GSEA showed that most related genes (CPT-I, CPT-II, SCD, and ACSL, etc.)…”

Section: Discussionmentioning

confidence: 99%

Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

et al. 2022

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The aim of this study was to investigate the effect of dietary protein level (soybean meal) on growth performance, flesh quality of grass carp, and the related molecular mechanisms. The results showed that appropriate dietary protein levels improved the growth performance, hardness, and pH of muscle while decreasing muscle crude lipid content and cooking loss and altering the antioxidant capacity and metabolic enzymes activities. In addition, appropriate dietary protein promoted the gene expression of myhc-1, myhc-4, myf5, myod, myog, and fgf6a, whereas inhibited that of myhc-7, myhc-2, mrf4, and mstn. Transcriptome profiling of muscle revealed that the flesh quality-specific differences were related to tight junctions and intramuscular fat (IMF) accumulation. GSEA showed that fatty acid metabolism and oxidative phosphorylation were downregulated in SM5 compared with SM1. To conclude, appropriate protein levels improved the growth and flesh quality by regulating muscle antioxidant capacity and gene expression of myhcs and fat metabolism-related signaling molecules.

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Section: Discussionmentioning

confidence: 99%

Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

et al. 2022

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“…A previous study demonstrated that the deficiency of AQP7 in adipose tissue leads to an increase in Gyk activity and intracellular glycerol content, with subsequent TAG accumulation in adipocytes (Hibuse et al, 2005). Similarly, we have previously shown that Gyk, despite its relatively low activity in white adipose tissue compared with liver and IBAT (Lin, 1977), is responsible for maintaining the supply of G3P for fatty acid esterification and TAG formation in epididymal adipose tissue from DEXA-treated rats (Ferreira et al, 2017) and in retroperitoneal adipose tissue from high-fat diet-fed mice (Buzelle et al, 2020). The radioisotopic dilution of the labeled glycerol could be the reason that we found decreased incorporation of U-[ 14 C]-glycerol into TAG of IBAT.…”

Section: Discussionmentioning

confidence: 75%

Glucocorticoids decrease thermogenic capacity and increase triacylglycerol synthesis by glycerokinase activation in the brown adipose tissue of rats

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Silva

Lautherbach

et al. 2022

Lipids

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Although it is well established that glucocorticoids inactivate thermogenesis and promote lipid accumulation in interscapular brown adipose tissue (IBAT), the underlying mechanisms remain unknown. We found that dexamethasone treatment (1 mg/kg) for 7 days in rats decreased the IBAT thermogenic activity, evidenced by its lower responsiveness to noradrenaline injection associated with reduced content of mitochondrial proteins, respiratory chain protein complexes, noradrenaline, and the β 3 -adrenergic receptor. In parallel, to understand better how dexamethasone increases IBAT lipid content, we also investigated the activity of the ATP citrate lyase (ACL), a key enzyme of de novo fatty acid synthesis, glucose-6-phosphate dehydrogenase (G6PD), a rate-limiting enzyme of the pentose phosphate pathway, and the three glycerol-3-P generating pathways: (1) glycolysis, estimated by 2-deoxyglucose uptake, (2) glyceroneogenesis, evaluated by phosphoenolpyruvate carboxykinase activity and pyruvate incorporation into triacylglycerol-glycerol, and (3) direct phosphorylation of glycerol, investigated by the content and activity of glycerokinase. Dexamethasone increased the mass and the lipid content of IBAT as well as plasma levels of glucose, insulin, non-esterified fatty acid, and glycerol. Furthermore, dexamethasone increased ACL and G6PD activities (79% and 48%, respectively). Despite promoting a decrease in the incorporation of U-[ 14 C]-glycerol into triacylglycerol ($54%), dexamethasone increased the content ($55%) and activity ($41%) of glycerokinase without affecting glucose uptake or glyceroneogenesis. Our data suggest that glucocorticoid administration reduces IBAT thermogenesis through sympathetic inactivation and stimulates glycerokinase activity and content, contributing to increased generation of glycerol-3-P, which is mostly used to esterify fatty acid and increase triacylglycerol content promoting IBAT whitening.

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“…There was no significant change in mRNA levels. 59 This study suggests that a possible reason for the difference is the downregulation of p-STAT3 expression in RWAT. This hypothesis is consistent with the results of this study.…”

Section: Food and Function Papermentioning

confidence: 80%

“…Moreover, studies have shown that the expression and activity of LPL vary in different tissues. The expression of LPL is upregulated in epididymal white adipose tissues (EWAT) in the HFD model, 58,59 but in retroperitoneal white adipose tissues (RWAT), LPL activity decreased significantly. There was no significant change in mRNA levels.…”

Section: Food and Function Papermentioning

confidence: 99%

Chinese chestnut shell polyphenol extract regulates the JAK2/STAT3 pathway to alleviate high-fat diet-induced, leptin-resistant obesity in mice

et al. 2023

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Activation of adipose tissue glycerokinase contributes to increased white adipose tissue mass in mice fed a high-fat diet

Cited by 5 publications

References 53 publications

Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

Glucocorticoids decrease thermogenic capacity and increase triacylglycerol synthesis by glycerokinase activation in the brown adipose tissue of rats

Chinese chestnut shell polyphenol extract regulates the JAK2/STAT3 pathway to alleviate high-fat diet-induced, leptin-resistant obesity in mice

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