Activation of adenylate cyclase in <i>cdc25</i> mutants of <i>Saccharomyces cerevisiae</i>

Pardo, Luis A.; Lazo, Pedro S.; Ramos, Sofı́a

doi:10.1016/0014-5793(93)80554-8

Cited by 17 publications

(15 citation statements)

References 25 publications

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“…We have previously shown that intracellular acidi¢cation causes an increase in the GTP/GDP ratio on the Ras proteins through inactivation of the Ras-GTPases, Ira1 and Ira2 [17]. In addition, Cdc25 and Ras are also involved in membrane targeting of adenylate cyclase [60,61]. As such, the ampli¢cation model would be consistent with several studies on the importance of Cdc25 and Ras-GTP for glucose-induced cAMP signalling [15,56^58].…”

Section: Intracellular Acidi¢cation Ampli¢es the Hexose Kinase-mediatsupporting

confidence: 87%

The role of hexose transport and phosphorylation in cAMP signalling in the yeast

Rolland

Wanke

Cauwenberg

et al. 2001

FEMS Yeast Research

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Section: Intracellular Acidi¢cation Ampli¢es the Hexose Kinase-mediatsupporting

confidence: 87%

The role of hexose transport and phosphorylation in cAMP signalling in the yeast

Rolland

Wanke

Cauwenberg

et al. 2001

FEMS Yeast Research

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“…Results obtained with cdc25 mutants can be explained by the fact that active Ras and therefore active Cdc25 is required for cAMP signalling by the Gpr1–Gpa2 system, but the signal does not run through Ras. Alternatively, inactivation of Cdc25 drastically reduces the amount of membrane‐bound adenylate cyclase (Pardo et al ., 1993). Deletion of the RAS genes also results in cytosolic localization of adenylate cyclase, but overexpression of Cdc25 in a ras1Δ ras2Δ bcy1Δ mutant again restores adenylate cyclase plasma membrane localization (Engelberg et al ., 1990).…”

Section: Is Ras Required For Signalling Through Adenylate Cyclase?mentioning

confidence: 99%

Novel sensing mechanisms and targets for the cAMP–protein kinase A pathway in the yeast Saccharomyces cerevisiae

Thevelein

Winde

1999

Molecular Microbiology

615

562

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The cAMP–protein kinase A (PKA) pathway in the yeast Saccharomyces cerevisiae plays a major role in the control of metabolism, stress resistance and proliferation, in particular in connection with the available nutrient conditions. Extensive information has been obtained on the core section of the pathway, i.e. Cdc25, Ras, adenylate cyclase, PKA, and on components interacting directly with this core section, such as the Ira proteins, Cap/Srv2 and the two cAMP phosphodiesterases. Recent work has now started to reveal upstream regulatory components and downstream targets of the pathway. A G‐protein‐coupled receptor system (Gpr1–Gpa2) acts upstream of adenylate cyclase and is required for glucose activation of cAMP synthesis in concert with a glucose phosphorylation‐dependent mechanism. Although a genuine signalling role for the Ras proteins remains unclear, they appear to mediate at least part of the potent stimulation of cAMP synthesis by intracellular acidification. Recently, several new targets of the PKA pathway have been discovered. These include the Msn2 and Msn4 transcription factors mediating part of the induction of STRE‐controlled genes by a variety of stress conditions, the Rim15 protein kinase involved in stationary phase induction of a similar set of genes and the Pde1 low‐affinity cAMP phosphodiesterase, which specifically controls agonist‐induced cAMP signalling. A major issue that remains to be resolved is the precise connection between the cAMP–PKA pathway and other nutrient‐regulated components involved in the control of growth and of phenotypic characteristics correlated with growth, such as the Sch9 and Yak1 protein kinases. Cln3 appears to play a crucial role in the connection between the availability of certain nutrients and Cdc28 kinase activity, but it remains to be clarified which nutrient‐controlled pathways control Cln3 levels.

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“…Several distinct functional domains have been identi¢ed in this protein. Ras2p and the GEF, Cdc25p, associate with adenylate cyclase in this membrane-bound complex and the localisation thereof to the plasma membrane is dependent on Cdc25p [211]. The central leucine-rich repeat domain was identi¢ed as the Ras-responsive region of the protein [209] and also found to interact physically with Ras2p [210].…”

Section: 112mentioning

confidence: 99%

The sensing of nutritional status and the relationship to filamentous growth inSaccharomyces cerevisiae

Gagiano

Bauer

Pretorius

2002

FEMS Yeast Research

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Heterotrophic organisms rely on the ingestion of organic molecules or nutrients from the environment to sustain energy and biomass production. Non-motile, unicellular organisms have a limited ability to store nutrients or to take evasive action, and are therefore most directly dependent on the availability of nutrients in their immediate surrounding. Such organisms have evolved numerous developmental options in order to adapt to and to survive the permanently changing nutritional status of the environment. The phenotypical, physiological and molecular nature of nutrient-induced cellular adaptations has been most extensively studied in the yeast Saccharomyces cerevisiae. These studies have revealed a network of sensing mechanisms and of signalling pathways that generate and transmit the information on the nutritional status of the environment to the cellular machinery that implements specific developmental programmes. This review integrates our current knowledge on nutrient sensing and signalling in S. cerevisiae, and suggests how an integrated signalling network may lead to the establishment of a specific developmental programme, namely pseudohyphal differentiation and invasive growth.

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Activation of adenylate cyclase in cdc25 mutants of Saccharomyces cerevisiae

Cited by 17 publications

References 25 publications

The role of hexose transport and phosphorylation in cAMP signalling in the yeast

The role of hexose transport and phosphorylation in cAMP signalling in the yeast

Novel sensing mechanisms and targets for the cAMP–protein kinase A pathway in the yeast Saccharomyces cerevisiae

The sensing of nutritional status and the relationship to filamentous growth inSaccharomyces cerevisiae

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