Activation of a Meiotic Checkpoint during Drosophila Oogenesis Regulates the Translation of Gurken through Chk2/Mnk

Abdu, Uri; Brodsky, Michael; Schüpbach, Trudi

doi:10.1016/s0960-9822(02)01165-x

Cited by 135 publications

(152 citation statements)

References 44 publications

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“…In particular, the pachytene checkpoint is triggered in meiocytes impaired in synapsis and recombination (Roeder and Bailis, 2000). It widely exists in many species, including S. cerevisiae, Drosophila, C. elegans, and mice, and leads to cell cycle arrest during the pachytene stage and cell death thereafter (Edelmann et al, 1996;McKee and Kleckner, 1997b;Pittman et al, 1998;Gartner et al, 2000;Roeder and Bailis, 2000;Abdu et al, 2002).…”

Section: Mof Is Required For Meiotic Progression In Ricementioning

confidence: 99%

“…Activation of the pachytene checkpoint leads to an arrest of the meiotic program that results in apoptosis. The pachytene checkpoint widely exists in yeast and animals, including Saccharomyces cerevisiae, Drosophila melanogaster, Caenorhabditis elegans, mice, and other mammals (Edelmann et al, 1996;McKee and Kleckner, 1997b;Pittman et al, 1998;Gartner et al, 2000;Abdu et al, 2002). However, it is not clear whether a similar pachytene checkpoint exists in plants because unlike yeast and animals that cause meiotic cell arrest or cell death, most plant meiotic mutants defective in SPO11 initiated recombination can complete meiosis but produce abnormal microspores Wijnker and Schnittger, 2013).…”

Section: Introductionmentioning

confidence: 99%

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MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice

Wang

Higgins

et al. 2016

Plant Cell

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Section: Mof Is Required For Meiotic Progression In Ricementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice

Wang

Higgins

et al. 2016

Plant Cell

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“…Delays in meiotic DSB repair lead to oocyte dorsal-ventral polarity defects in the developing embryos of females due to the meiotic DSB repair checkpoint (Ghabrial and Schupbach 1999;Abdu et al 2002). We reasoned that if DSB repair occurs by NHEJ in mei-218; spn-D and mei-218; spn-B females, then the frequency of abnormal embryos (a readout for checkpoint activity) laid by these mothers would be reduced.…”

Section: Repair Of Dsbs In Mei-218; Spn-d Depends On Ligase4mentioning

confidence: 99%

Multiple Barriers to Nonhomologous DNA End Joining During Meiosis inDrosophila

Joyce¹,

Paul

Chen

et al. 2012

Genetics

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Repair of meiotic double-strand breaks (DSBs) uses the homolog and recombination to yield crossovers while alternative pathways such as nonhomologous end joining (NHEJ) are suppressed. Our results indicate that NHEJ is blocked at two steps of DSB repair during meiotic prophase: first by the activity of the MCM-like protein MEI-218, which is required for crossover formation, and, second, by Rad51-related proteins SPN-B (XRCC3) and SPN-D (RAD51C), which physically interact and promote homologous recombination (HR). We further show that the MCM-like proteins also promote the activity of the DSB repair checkpoint pathway, indicating an early requirement for these proteins in DSB processing. We propose that when a meiotic DSB is formed in the absence of both MEI-218 and SPN-B or SPN-D, a DSB substrate is generated that can enter the NHEJ repair pathway. Indeed, due to its high error rate, multiple barriers may have evolved to prevent NHEJ activity during meiosis.

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“…Later work showed that vas-null females produce rare tumorous egg chambers in which nurse cells and oocytes have not differentiated, and also rare egg chambers with supernumerary, misplaced, or no oocytes, suggesting that Vas may also function in restricting cell fate during germline stem cell (GSC) differentiation (Styhler et al 1998;Tomancak et al 1998). A DNA damage checkpoint that functions in the oocyte during meiotic prophase, after the 16-cell cyst has formed, regulates Vas activity (Ghabrial and Schü pbach 1999;Abdu et al 2002;Findley et al 2003;Theurkauf et al 2006;Chen et al 2007;Pane et al 2007). Vas binds to eIF5B, an essential translation initiation factor that functions in ribosomal subunit joining (Carrera et al 2000;Pestova et al 2000).…”

mentioning

confidence: 99%

Vasa promotes Drosophila germline stem cell differentiation by activating mei-P26 translation by directly interacting with a (U)-rich motif in its 3′ UTR

Liu

Han²,

Lasko³

2009

Genes Dev.

104

105

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Vasa (Vas) is a DEAD-box RNA-binding protein required in Drosophila at several steps of oogenesis and for primordial germ cell (PGC) specification. Vas associates with eukaryotic initiation factor 5B (eIF5B), and this interaction has been implicated in translational activation of gurken mRNA in the oocyte. Vas is expressed in all ovarian germline cells, and aspects of the vas-null phenotype suggest a function in regulating the balance between germline stem cells (GSCs) and their fate-restricted descendants. We used a biochemical approach to recover Vas-associated mRNAs and obtained mei-P26, whose product represses microRNA activity and promotes GSC differentiation. We found that vas and mei-P26 mutants interact, and that mei-P26 translation is substantially reduced in vas mutant cells. In vitro, Vas protein bound specifically to a (U)-rich motif in the mei-P26 39 untranslated region (UTR), and Vas-dependent regulation of GFP-mei-P26 transgenes in vivo was dependent on the same (U)-rich 39 UTR domain. The ability of Vas to activate mei-P26 expression in vivo was abrogated by a mutation that greatly reduces its interaction with eIF5B. Taken together, our data support the conclusion that Vas promotes germ cell differentiation by directly activating mei-P26 translation in early-stage committed cells.[Keywords: Oogenesis; RNA-binding protein; DEAD-box; patterning; development] Supplemental material is available at http://www.genesdev.org.

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Activation of a Meiotic Checkpoint during Drosophila Oogenesis Regulates the Translation of Gurken through Chk2/Mnk

Abstract: DmChk2 is a transducer of the meiotic checkpoint in flies that is activated by unrepaired double-strand DNA breaks. Activation of DmChk2 in this specific checkpoint affects a cell cycle regulator as well as mRNA translation.

Cited by 135 publications

References 44 publications

MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice

MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice

Multiple Barriers to Nonhomologous DNA End Joining During Meiosis inDrosophila

Vasa promotes Drosophila germline stem cell differentiation by activating mei-P26 translation by directly interacting with a (U)-rich motif in its 3′ UTR

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