Activation in stored platelet concentrates: correlation between membrane expression of P‐selectin, glycoprotein IIb/IIIa, and beta‐ thromboglobulin release

Rinder, Henry M.; Snyder, Edward L.; Bonan, Jayne L.; Napychank, PA; Malkus, H; Smith, Brian R.

doi:10.1046/j.1537-2995.1993.33193142305.x

Cited by 119 publications

(111 citation statements)

References 21 publications

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“…1 A ) in experiments where monocytes were incubated with unstimulated platelets. These small increases may have been due to spontaneous activation of the platelets with longer ex vivo incubation periods (34). In contrast, platelets activated by thrombin induced significant ( P Ͻ 0.01) release of MCP-1 compared with levels found when monocytes were incubated in the absence of platelets or with unactivated platelets.…”

Section: Resultsmentioning

confidence: 89%

Activated platelets signal chemokine synthesis by human monocytes.

et al. 1996

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Human blood monocytes adhere rapidly and for prolonged periods to activated platelets that display P-selectin, an adhesion protein that recognizes a specific ligand on leukocytes, P-selectin glycoprotein-1. We previously demonstrated that P-selectin regulates expression and secretion of cytokines by stimulated monocytes when it is presented in a purified, immobilized form or by transfected cells. Here we show that thrombin-activated platelets induce the expression and secretion of monocyte chemotactic protein-1 and IL-8 by monocytes. Enhanced monokine synthesis requires engagement of P-selectin glycoprotein-1 on the leukocyte by P-selectin on the platelet. Secretion of the chemokines is not, however, directly signaled by P-selectin; instead, tether-

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Section: Resultsmentioning

confidence: 89%

Activated platelets signal chemokine synthesis by human monocytes.

et al. 1996

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“…Additionally, C3a, C4a and β-thromboglobulin were reduced after filtration [17] and an increased platelet recovery of filtered platelet concentrates has been observed [18]. Thus, leukocyte reduction results in improved quality [19], but also in the loss of 10–20% of platelets [6, 12]. This loss may increase, if clogging phenomena of the filter or previous platelet activation occur [20, 21].…”

Section: Discussionmentioning

confidence: 99%

“…Platelet membrane glycoproteins were analyzed by flow cytometry (FACS Scan with LYSIS II, Becton Dickinson, Heidelberg, Germany) after incubation with fluorescence-labeled antibodies against CD41a, CD42b, CD62 and CD63 (Becton Dickinson) as previously described [12]. Furthermore, leukocyte subsets were determined by flow cytometry in donors of both groups (n = 15 each) before and after apheresis according to standard methods [13].…”

Section: Methodsmentioning

confidence: 99%

Quality and Safety of& ;Platelet Apheresis Concentrates Produced with a New Leukocyte Reduction System

et al. 1998

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Objectives: Contaminating white blood cells (WBC) in apheresis platelet concentrates (PC) can cause a variety of adverse effects after platelet transfusion. To obtain PCs with low WBC contamination, a new leukoreduction system (LRS) utilizing ‘fluidized particle bed’ technology has recently been introduced. Methods: We prospectively examined the effect of LRS apheresis on the donor, the quality of the resulting PCs (n = 120), and the platelet increment in the corresponding recipients. Conventionally prepared apheresis PCs served as control group (n = 27). Platelet glycoproteins were examined by flow cytometry. Results: In LRS apheresis, we observed no serious adverse effects on the donors, but the postdonation absolute lymphocyte counts were reduced from 1,787±505/μl to 1,405±383/μl (p < 0.001). Comparable results were seen in non-LRS donors. The collection efficiency of the LRS procedures was 50.0±7.6%, resulting in a yield of 4.3±1.0 × 10¹¹ platelets/PC. In flow cytometry, platelet glycoproteins in LRS PCs were not elevated: mean fluorescence of CD62 (6±4) or CD63 (9±3) in comparison with non-LRS PCs (mean fluorescence of CD62: 7±4, CD63: 8±3). Median leukocyte contamination of the LRS PCs was 0.41 × 10⁵ (range 0.07–8.5) WBCs/unit. In 43 recipients, the 24-hour corrected count increments after transfusion of LRS PCs (12,530±8,761) were essentially the same as those of 20 recipients of non-LRS PCs (13,133±9,812; p = 0.75). Conclusions: LRS apheresis appears to be a safe procedure, which produced effective PCs with few contaminating leukocytes. With new apheresis technology, filtration of PCs may become superfluous.

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“…Decreased swirling is one of the most simple and inexpensive methods available to assess platelet activation [4], however this determination is subjective. More objective procedures include the detection and quantitation of antigens exposed during platelet degranulation [7]. Degranulation involves the externalization of alpha granule contents onto the platelet surface and the exposure of antigens normally confined within the granules of resting platelets [8, 9, 10].…”

Section: Introductionmentioning

confidence: 99%

“…Following platelet activation and granule release, P-selectin is also present on the external surface of platelets [8, 9, 10]. P-selectin exposure can be detected by flow cytometry and used as a measure of platelet activation [1, 2, 6, 7, 12]. …”

Section: Introductionmentioning

confidence: 99%

Does Hyperconcentration Result in Platelet Activation?

Gollehon¹,

King²,

Craig³

1998

Vox Sanguinis

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Background and Objectives: Hyperconcentration of platelets may lead to platelet activation and loss of platelet function. Materials and Methods: Platelet activation following hyperconcentration was assessed using flow-cytometric detection of platelet P-selectin expression and platelet swirling. Results: Platelet hyperconcentration led to a minimal increase in P-selectin expression and no difference in platelet swirling. Conclusion: Hyperconcentration was not associated with a clinically significant change in platelet activation and had no significant effect on platelet quality as detected by pH and platelet yield.

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Activation in stored platelet concentrates: correlation between membrane expression of P‐selectin, glycoprotein IIb/IIIa, and beta‐ thromboglobulin release

Cited by 119 publications

References 21 publications

Activated platelets signal chemokine synthesis by human monocytes.

Activated platelets signal chemokine synthesis by human monocytes.

Quality and Safety of& ;Platelet Apheresis Concentrates Produced with a New Leukocyte Reduction System

Does Hyperconcentration Result in Platelet Activation?

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