Activation effect of Ganoderma lucidum polysaccharides liposomes on murine peritoneal macrophages

Liu, Zhenguang; Xing, Jie; Huang, Yee; Bo, Ruonan; Zheng, Simin; Luo, Li; Niu, Yale; Zhang, Yan; Hu, Yuanliang; Liu, Jiaguo; Wu, Yi; Wang, Deyun

doi:10.1016/j.ijbiomac.2015.10.088

Cited by 46 publications

(30 citation statements)

References 22 publications

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“…After washing, the remaining cells were more than 90% macrophages. Macrophages were analyzed at this step or cultured in DMEM medium and maintained for several days [21, 22]. The Animal Committee of The Second Military University approved all mouse experiments.…”

Section: Methodsmentioning

confidence: 99%

Gelsolin Inhibits the Inflammatory Process Induced by LPS

Cheng

Liu

et al. 2017

Cell Physiol Biochem

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Background/Aims: Endotoxemia is a life-threatening situation that signifies a key challenge in the field of intensive care medicine. Proinflammatory mediators produced by macrophages play a key role in endotoxemia. Gelsolin (GSN) is involved in the process of inflammation. Methods: IL-6 and TNF-α in the supernatant were measured with an ELISA kit. NO production was assessed by measurement of nitrite concentration with the Griess assay. si-RNA directed against GSN (si-GSN) was transfected by Lipofectamine. Results: LPS decreased the levels of GSN. Recombinant GSN inhibited the cytokines induced by LPS and rescued mice from LPS-induced death, and si-GSN increased death in the LPS-pretreated mice. Conclusion: GSN exhibited a protective role in endotoxemia.

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Section: Methodsmentioning

confidence: 99%

Gelsolin Inhibits the Inflammatory Process Induced by LPS

Cheng

Liu

et al. 2017

Cell Physiol Biochem

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“…The polysaccharide composition of G. lucidum (GLP) includes several monomers (glucose, mannose, galactose, fucose, xylose, rhamnose and arabinose) that are described to contribute significantly to its immunomodulatory, antioxidant, antitumour and antibacterial properties Liu et al, 2016a). Among the terpenes, the triterpenes (ganoderic acids, ganoderenic acids, ganoderols and lucideric acids) are the most abundant ones, having been reported to be responsible for its antitumour, antiinflammatory, antioxidant, anti-hepatitis, antimalarial, hypoglycemic, antimicrobial and anti-HIV-1 properties (Baby et al, 2015;Bishop et al, 2015;Grienke et al, 2015;Van Nguyen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits

Taofiq

Heleno

Calhelha

et al. 2017

Food and Chemical Toxicology

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a b s t r a c tGanoderma lucidum was characterized in terms of nutritional value and chemical composition. Thereafter, ethanolic Soxhlet extracts were evaluated for antioxidant, anti-inflammatory, antityrosinase, antimicrobial and cytotoxic effects, and further characterized in terms of phenolic acids, polysaccharides and triterpenoids. Finally, the obtained extracts were tested as cosmeceutical ingredients. G. lucidum proved to be a source of macronutrients and important bioactive compounds such as terpenoids, specially triterpenoids, and polysaccharides. In the extracts, ganoderic acids C2, A and H were the most abundant triterpenic acids and protocatechuic, p-hydroxibenzoic and syringic acids the identified phenolics. The developed cosmeceutical formulation preserved the extract bioactivities, presented a lightyellow colour and a pH of 4.6, which is considered appropriate for cosmeceutical's design. Behind the important nutritional/bioactive composition of G. lucidum, a potential towards its valorisation in the field of cosmeceuticals is foreseeable, as deduced from the bioactivities of its ethanolic extract and preservation in the tested formulation.

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“…On the other side, Ganoderma spp. extracts and metabolites (derived from wild and/or traditionally cultivated basidiocarps) express strong immunomodulatory characteristics and present an effective modifiers of different biological processes (Lin et al, 2006b; Boh, 2013; Shi et al, 2013; Liu et al, 2016a,b). According to available literature, there is no knowledge about the immunomodulatory potential of crude extracts of G. lucidum basidiocarps cultivated on any alternative and environmentally friendly substrate.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of extract ofGanoderma lucidumbasidiocarps cultivated on alternative substrate

Nedeljković

Ćilerdžić

Zmijanjac

et al. 2019

Preprint

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24Background: Ganoderma lucidum is a medicinal mushroom exhibiting numerous health 25 benefits primarily based on strong immunostimulatory effects. The aim of the study was 26 to investigate if there were differences in effects of extracts of commercially (GC) and 27 alternatively (wheat straw) (GA) cultivated G. lucidum basidiocarps on properties of 28 peritoneal macrophages (PM) and monocyte-derived dendritic cells (MoDCs). 29 Methods: Differences in immunomodulatory effects of GC/GA extracts were studied. 30 Viability of treated PMs, their adhesive and phagocytic capability and capacity to produce 31 reactive oxigen species (ROS) and NO was tested. Immature MoDCs generated from 32 human monocytes were treated with poly I:C (10.0 µg/ml) and loxoribine (34.0 µg/ml), a 33 selective TLR3 and TLR7 agonists, respectively, and with/without GC/GA extract (100.0 34 µg/ml). The effect of each combination on phenotypic properties, cytokines production by 35 MoDCs, and their proliferation and Th polarizing capacity was studied. 36 Results: GA extract stronger stimulated metabolic and phagocytic activity of PMs, their 37 adhesion capability and ability to produce ROS and NO compared to GC. Both tested 38 extracts significantly increased allostimulatory and Th1 polarization capacity of 39 simultaneous TLR3 and TLR7-activated MoDCs, but GA extract was more effective. 40 Discussion: The extract of alternatively cultivated G. lucidum basidiocarps increased 41 production of ROS and NO by TLR4 stimulated PMs and up-regulated production of 42 certain cytokines as well as allostimulatory and Th1 polarization capacity of MoDCs. GA 43 extract could be a potent immunostimulatory agent for activation of MoDCs with 44 simultaneous engagement of TLRs that seems to be a promising strategy for preparation 45 of DC-based anti-tumor vaccines. 46 47

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Activation effect of Ganoderma lucidum polysaccharides liposomes on murine peritoneal macrophages

Cited by 46 publications

References 22 publications

Gelsolin Inhibits the Inflammatory Process Induced by LPS

Gelsolin Inhibits the Inflammatory Process Induced by LPS

The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits

Immunomodulatory effects of extract ofGanoderma lucidumbasidiocarps cultivated on alternative substrate

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