2019
DOI: 10.1016/j.immuni.2019.06.024
|View full text |Cite
|
Sign up to set email alerts
|

Activation Dynamics and Immunoglobulin Evolution of Pre-existing and Newly Generated Human Memory B cell Responses to Influenza Hemagglutinin

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

18
133
0

Year Published

2019
2019
2023
2023

Publication Types

Select...
5
2
1

Relationship

1
7

Authors

Journals

citations
Cited by 115 publications
(157 citation statements)
references
References 75 publications
18
133
0
Order By: Relevance
“…As a comparator non-malaria antigen, we used influenza surface glycoprotein hemagglutinin (HA)-the principal target of influenza-specific neutralizing antibodies. Using the Meso Scale Discovery (MSD) assay, which is an electrochemiluminescent-based ELISA replacement assay (22), we found in the Mali cohort a high prevalence of serum IgG reactivity specific for the influenza A subtypes H1N1 or H3N2, which have been reported to circulate in Mali (23) ( Fig. S1).…”
Section: Confirmation Of Specificity Of Pfama1/pfmsp1 and Ha B Cell Pmentioning
confidence: 99%
See 2 more Smart Citations
“…As a comparator non-malaria antigen, we used influenza surface glycoprotein hemagglutinin (HA)-the principal target of influenza-specific neutralizing antibodies. Using the Meso Scale Discovery (MSD) assay, which is an electrochemiluminescent-based ELISA replacement assay (22), we found in the Mali cohort a high prevalence of serum IgG reactivity specific for the influenza A subtypes H1N1 or H3N2, which have been reported to circulate in Mali (23) ( Fig. S1).…”
Section: Confirmation Of Specificity Of Pfama1/pfmsp1 and Ha B Cell Pmentioning
confidence: 99%
“…To more directly confirm the antigen specificity of the B cell probes, we sorted single probe-positive B cells from adult Malian PBMC samples (gating strategy in Fig. 1 A-D) and amplified the BCR using reverse transcription and PCR followed by Sanger sequencing (22,28). Heavy chain and corresponding light chain sequences of four Pf-and HA-probe binding B cells were chosen for cloning and expression as recombinant human monoclonal antibodies (hmAb).…”
Section: Confirmation Of Specificity Of Pfama1/pfmsp1 and Ha B Cell Pmentioning
confidence: 99%
See 1 more Smart Citation
“…In humans, both switched immunoglobulin (swIg + ) and immunoglobulin M (IgM + ) memory cells have been described, as well as "atypical" MBCs that express FCRL4 or FCRL5 and/or lack expression of the canonical MBC surface marker CD27 (2,3). Various activated B cell subsets, which appear transiently after infection or vaccination, have also been reported (4)(5)(6). In mice, MBCs can be subcategorized based on their expression of Ig isotype as well as other surface markers, such as CD80, PD-L2, and CD73 (2,7).…”
mentioning
confidence: 99%
“…Circulating MBCs adapted to the H3 head domain were detected within ∼4 wk of symptom onset. Evidence that HA-reactive MBCs with increased levels of somatic hypermutation are formed many months after HA vaccination in humans indicates that affinity maturation of MBCs continues long after any early switch by GCs to predominantly plasma cell production (Andrews et al 2019;Matsuda et al 2019).…”
Section: B-cell Response To Seasonal Influenza Infectionmentioning
confidence: 99%