Activation and turnover of the plant immune signaling kinase BIK1: a fine balance

Dias, Márcia Gonçalves; Soleimani, Faranak; Monaghan, Jacqueline

doi:10.1042/ebc20210071

Cited by 7 publications

(2 citation statements)

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“…CPK28 phosphorylates the E3 ubiquitin ligases PLANT U-BOX 25 (PUB25) and PUB26, enhancing their ability to poly-ubiquitinate the RLCK BOTRYTIS INDUCED KINASE 1 (BIK1), a common substrate of multiple receptors and a critical signaling node in plant immunity (Monaghan et al, 2014;Wang et al, 2018;DeFalco & Zipfel, 2021). The CPK28-PUB25/26 regulatory module buffers BIK1 protein accumulation to optimize immune output (Gonçalves Dias et al, 2022). In the current study, we aimed to identify additional CPK28 binding partners in Arabidopsis using a proteomics approach.…”

Section: Introductionmentioning

confidence: 99%

Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening inArabidopsis thaliana

Dias,

Doss,

Rawat

et al. 2023

Preprint

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SummaryThe calcium-dependent protein kinase CPK28 is a regulator of immune homeostasis in multiple plant species. Here, we used a proteomics approach to uncover CPK28-associated proteins. We found that CPK28 associates with subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39, and trans-phosphorylates RAF26 and RAF39. Metazoan Raf kinases function in mitogen-activated protein kinase (MAPK) cascades as MAPK kinase kinases (MKKKs). Although Raf-like kinases share some features with MKKKs, we found that MRK1, RAF26, and RAF39 are unable to trans-phosphorylate any of the 10 Arabidopsis MKKs. We show that MRK1, RAF26, and RAF39 localize to the cytosol and endomembranes, and we define redundant roles for these kinases in stomatal opening, immune-triggered reactive oxygen species (ROS) production, and resistance to a bacterial pathogen. Overall, our study suggests that C7 Raf-like kinases associate with and are phosphorylated by CPK28, function redundantly in stomatal immunity, and possess substrate specificities distinct from canonical MKKKs.

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Section: Introductionmentioning

confidence: 99%

Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening inArabidopsis thaliana

Dias,

Doss,

Rawat

et al. 2023

Preprint

Self Cite

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“…In Arabidopsis thaliana (Arabidopsis), subfamily VII RLCKs (RLCK‐VIIs) BOTRYTIS‐INDUCED KINASE 1 (BIK1) and homologue PBS‐Like 1 (PBL1) are rapidly phosphorylated and activated by multiple PRR complexes to initiate intracellular signalling (Couto & Zipfel, 2016 ; Liang & Zhou, 2018 ). BIK1 associates with numerous immune signalling proteins including RESPIRATORY BURST OXIDASE HOMOLOGUE D (RBOHD), cyclic nucleotide gated ion channels CNGC2/CNGC4, and calcium‐dependent protein kinases (CDPKs) (Gonçalves Dias et al, 2022 ; Sun & Zhang, 2020 ). bik1 pbl1 loss‐of‐function plants display weakened defence responses and increased susceptibility to bacterial and fungal pathogens (Lu et al, 2010 ; Veronese et al, 2006 ; Zhang et al, 2010 ).…”

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confidence: 99%

Characterization of a foxtail mosaic virus vector for gene silencing and analysis of innate immune responses in Sorghum bicolor

Bredow

Natukunda

Beernink

et al. 2022

Molecular Plant Pathology

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Sorghum is vulnerable to many biotic and abiotic stresses, which cause considerable yield losses globally. Efforts to genetically characterize beneficial sorghum traits, including disease resistance, plant architecture, and tolerance to abiotic stresses, are ongoing. One challenge faced by sorghum researchers is its recalcitrance to transformation, which has slowed gene validation efforts and utilization for cultivar development. Here, we characterize the use of a foxtail mosaic virus (FoMV) vector for virus‐induced gene silencing (VIGS) by targeting two previously tested marker genes: phytoene desaturase (PDS) and ubiquitin (Ub). We additionally demonstrate VIGS of a subgroup of receptor‐like cytoplasmic kinases (RLCKs) and report the role of these genes as positive regulators of early defence signalling. Silencing of subgroup 8 RLCKs also resulted in higher susceptibility to the bacterial pathogens Pseudomonas syringae pv. syringae (B728a) and Xanthomonas vasicola pv. holcicola, demonstrating the role of these genes in host defence against bacterial pathogens. Together, this work highlights the utility of FoMV‐induced gene silencing in the characterization of genes mediating defence responses in sorghum. Moreover, FoMV was able to systemically infect six diverse sorghum genotypes with high efficiency at optimal temperatures for sorghum growth and therefore could be extrapolated to study additional traits of economic importance.

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Conservation and divergence of flg22, pep1 and nlp20 in activation of immune response and inhibition of root development

Huang

Cui

et al. 2023

Plant Science

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Activation and turnover of the plant immune signaling kinase BIK1: a fine balance

Cited by 7 publications

References 83 publications

Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening inArabidopsis thaliana

Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening inArabidopsis thaliana

Characterization of a foxtail mosaic virus vector for gene silencing and analysis of innate immune responses in Sorghum bicolor

Conservation and divergence of flg22, pep1 and nlp20 in activation of immune response and inhibition of root development

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