2022
DOI: 10.3390/antiox11081616
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Activation and Inhibition of Human Matrix Metalloproteinase-9 (MMP9) by HOCl, Myeloperoxidase and Chloramines

Abstract: Matrix metalloproteinase-9 (MMP9, gelatinase B) plays a key role in the degradation of extracellular-matrix (ECM) proteins in both normal physiology and multiple pathologies, including those linked with inflammation. MMP9 is excreted as an inactive proform (proMMP9) by multiple cells, and particularly neutrophils. The proenzyme undergoes subsequent processing to active forms, either enzymatically (e.g., via plasmin and stromelysin-1/MMP3), or via the oxidation of a cysteine residue in the prodomain (the “cyste… Show more

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Cited by 10 publications
(12 citation statements)
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References 75 publications
(133 reference statements)
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“…Next, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), amino acid and protein chloramines were prepared as described previously [12,58]. Thus, HEPES-chloramines (HEPES-CA), Lys-(Lys-CA) and taurine-chloramines (Tau-CA) were prepared by adding HOCl to a fivefold molar excess of HEPES, Nα-acetyl-Lys and taurine, with the parent compounds present at 1 M, 100 mM, and 100 mM respectively, in 5 mM sodium phosphate buffer (pH 7.4).…”
Section: Formation and Quantification Of Chloraminesmentioning
confidence: 99%
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“…Next, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), amino acid and protein chloramines were prepared as described previously [12,58]. Thus, HEPES-chloramines (HEPES-CA), Lys-(Lys-CA) and taurine-chloramines (Tau-CA) were prepared by adding HOCl to a fivefold molar excess of HEPES, Nα-acetyl-Lys and taurine, with the parent compounds present at 1 M, 100 mM, and 100 mM respectively, in 5 mM sodium phosphate buffer (pH 7.4).…”
Section: Formation and Quantification Of Chloraminesmentioning
confidence: 99%
“…HSA-chloramines (HSA-CA), human plasma fibronectin-chloramines (FN-CA), laminin-111-chloramines (laminin-CA) and murine basement membrane extract chloramines (BME-CA) were generated by adding a 50-fold molar excess of HOCl over the protein concentration (40 µM HSA, 26 µM fibronectin, 14 µM laminin-111, 20 µM basement membrane extract based on the molecular mass of laminin). Chloramine concentrations were determined by quantifying the oxidation of 5-thio-2-nitrobenzoic acid (TNB) to 5,5 -dithiobis-(2-nitrobenzoic acid) (DTNB) as described previously [12,58]. TNB was generated from DTNB by treatment of 1.3 mM DTNB with 50 mM NaOH for 15 min at 21 • C; this material was then diluted 1:50 in PBS buffer.…”
Section: Formation and Quantification Of Chloraminesmentioning
confidence: 99%
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