Activating macrophages for enhanced osteogenic and bactericidal performance by Cu ion release from micro/nano-topographical coating on a titanium substrate

Huang, Qianli; Ouyang, Zhengxiao; Tan, Yan; Wu, Hong; Liu, Yong

doi:10.1016/j.actbio.2019.09.030

Cited by 128 publications

(75 citation statements)

References 53 publications

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“…A similar expression profile was observed when macrophages were seeded onto a copper containing bioceramic coating on a titanium substrate. In this case, the expression of the pro-inflammatory markers iNOS and NFκB were upregulated and pro-inflammatory cytokines were upregulated when releasing 12 and 22 μM copper ions 37 . However, when lower concentrations (between 1 and 5 μM) of copper ions were released from a copper doped Ti6Al4V alloy the expression of pro-inflammatory markers IL-1β, IL-6, TNF-α and CCR7 were downregulated and increased the expression of IL-10 and CD206 22 , 38 .…”

Section: Discussionmentioning

confidence: 90%

Evaluation of the immunomodulatory effects of cobalt, copper and magnesium ions in a pro inflammatory environment

Díez-Tercero

Delgado

Bosch-Rué

et al. 2021

Sci Rep

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Biomaterials and scaffolds for Tissue Engineering are widely used for an effective healing and regeneration. However, the implantation of these scaffolds causes an innate immune response in which the macrophage polarization from M1 (pro-inflammatory) to M2 (anti-inflammatory) phenotype is crucial to avoid chronic inflammation. Recent studies have showed that the use of bioactive ions such as cobalt (Co2+), copper (Cu2+) and magnesium (Mg2+) could improve tissue regeneration, although there is limited evidence on their effect on the macrophage response. Therefore, we investigated the immunomodulatory potential of Co2+, Cu2+ and Mg2+ in macrophage polarization. Our results indicate that Mg2+ and concentrations of Cu2+ lower than 10 μM promoted the expression of M2 related genes. However, higher concentrations of Cu2+ and Co2+ (100 μM) stimulated pro-inflammatory marker expression, indicating a concentration dependent effect of these ions. Furthermore, Mg2+ were able to decrease M1 marker expression in presence of a mild pro-inflammatory stimulus, showing that Mg2+ can be used to modulate the inflammatory response, even though their application can be limited in a strong pro-inflammatory environment.

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Section: Discussionmentioning

confidence: 90%

Evaluation of the immunomodulatory effects of cobalt, copper and magnesium ions in a pro inflammatory environment

Díez-Tercero

Delgado

Bosch-Rué

et al. 2021

Sci Rep

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“…Moreover, persistent low inflammation caused by the high activity of Ag may increase the incidence of IAI (Croes et al, 2018). In addition, Cu ions that can be used as a coating promoted the polarization of inflammatory macrophages both in vivo and in vitro and improved the killing effect of materials on MRSA (Huang et al, 2019;Liu et al, 2019). The relationship between copper antibacterial alloys in IAI and immunity has not yet been reported.…”

Section: Discussionmentioning

confidence: 99%

Recent Advances in Research on Antibacterial Metals and Alloys as Implant Materials

Jiao

Zhang

et al. 2021

Front. Cell. Infect. Microbiol.

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Implants are widely used in orthopedic surgery and are gaining attention of late. However, their use is restricted by implant-associated infections (IAI), which represent one of the most serious and dangerous complications of implant surgeries. Various strategies have been developed to prevent and treat IAI, among which the closest to clinical translation is designing metal materials with antibacterial functions by alloying methods based on existing materials, including titanium, cobalt, tantalum, and biodegradable metals. This review first discusses the complex interaction between bacteria, host cells, and materials in IAI and the mechanisms underlying the antibacterial effects of biomedical metals and alloys. Then, their applications for the prevention and treatment of IAI are highlighted. Finally, new insights into their clinical translation are provided. This review also provides suggestions for further development of antibacterial metals and alloys.

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“…The Cu-containing coatings on Ti implants are found to polarize macrophages to the M1 phenotype, stimulate the release of proinflammatory cytokines, [139] and inhibit the release of antiinflammatory cytokines (IL-10 and IL-4) by activating Cu-transport signaling in macrophages. [140,141] The in vivo test shows the enhanced expression levels of M1 surface marker CD11c at the implant/bone tissue interface. [141] Therefore, it is considered that the Cu-containing coating can create a proper inflammatory microenvironment to favor the osteogenic differentiation of osteoblast-like cells and osteogenesis in vivo.…”

Section: Cu-incorporated Coatingmentioning

confidence: 99%

“…[140,141] The in vivo test shows the enhanced expression levels of M1 surface marker CD11c at the implant/bone tissue interface. [141] Therefore, it is considered that the Cu-containing coating can create a proper inflammatory microenvironment to favor the osteogenic differentiation of osteoblast-like cells and osteogenesis in vivo.…”

Section: Cu-incorporated Coatingmentioning

confidence: 99%

Biofunctional Elements Incorporated Nano/Microstructured Coatings on Titanium Implants with Enhanced Osteogenic and Antibacterial Performance

Zhao

et al. 2020

Adv Healthcare Materials

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Bone fracture is prevalent among athletes and senior citizens and may require surgical insertion of bone implants. Titanium (Ti) and its alloys are widely used in orthopedics due to its high corrosion resistance, good biocompatibility, and modulus compatible with natural bone tissues. However, bone repair and regrowth are impeded by the insufficient intrinsic osteogenetic capability of Ti and Ti alloys and potential bacterial infection. The physicochemical properties of the materials and nano/microstructures on the implant surface are crucial for clinical success and loading with biofunctional elements such as Sr, Zn, Cu, Si, and Ag into nano/microstructured TiO 2 coating has been demonstrated to enhance bone repair/regeneration and bacterial resistance of Ti implants. In this review, recent advances in biofunctional element-incorporated nano/microstructured coatings on Ti and Ti alloy implants are described and the prospects and limitations are discussed.

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Activating macrophages for enhanced osteogenic and bactericidal performance by Cu ion release from micro/nano-topographical coating on a titanium substrate

Cited by 128 publications

References 53 publications

Evaluation of the immunomodulatory effects of cobalt, copper and magnesium ions in a pro inflammatory environment

Evaluation of the immunomodulatory effects of cobalt, copper and magnesium ions in a pro inflammatory environment

Recent Advances in Research on Antibacterial Metals and Alloys as Implant Materials

Biofunctional Elements Incorporated Nano/Microstructured Coatings on Titanium Implants with Enhanced Osteogenic and Antibacterial Performance

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