Keywords: Anaerobic sludge digester supernatant, SBR, simultaneous nitrifi cation and denitrifi cation (SND).Abstract: In this study, the dependence between volumetric exchange rate (n) in an SBR (Sequencing Batch Reactor) with a modifi ed cycle and simultaneous nitrifi cation and denitrifi cation (SND) effi ciency during the treatment of anaerobic sludge digester supernatant was determined. In the SBR cycle alternating three aeration phases (with limited dissolved oxygen (DO) concentration up to 0.7 mg O 2 /L) and two mixing phases were applied. The lengths of each aeration and mixing phases were 4 and 5.5 h, respectively. Independently of n, a total removal of ammonium was achieved. However, at n = 0.1 d -1 and n = 0.3 d -1 nitrates were the main product of nitrifi cation, while at n = 0.5 d -1, both nitrates and nitrites occurred in the effl uent. Under these operational conditions, despite low COD/N (ca. 4) ratio in the infl uent, denitrifi cation in activated sludge was observed. A higher denitrifi cation effi ciency at n = 0.5 d -1 (51.3%) than at n = 0.1 d -1 (7.8%) indicated that n was a crucial factor infl uencing SND via nitrite and nitrate in the SBR with a low oxygen concentration in aeration phases.