Activated sludge monitoring with combined respirometric–titrimetric measurements

Gernaey, Krist V.; Petersen, B.; Ottoy, Jean–Pierre; Vanrolleghem, Peter A.

doi:10.1016/s0043-1354(00)00366-3

Cited by 68 publications

(58 citation statements)

References 40 publications

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“…For the experiments with acetate, a linear increase of BOD st was observed when increasing amounts of acetate were added to the respirometer (data not shown; see Gernaey et al, 2001). The slope of this curve represents …”

Section: Experiments With Carbon Sources (Acetate and Dextrose)mentioning

confidence: 92%

“…Results of these experiments can be found in Gernaey et al (2001), Petersen (2000), and Petersen et al (2000Petersen et al ( , 2001. The results described in this paper focus on the degradation of organic carbon sources by activated sludge.…”

Section: Data Setmentioning

confidence: 99%

“…A detailed description of the interpretation of the respirometric data can be found in Gernaey et al (2001).…”

Section: Data Interpretationmentioning

confidence: 99%

“…When ammonium is added to an activated sludge sample, nitri®cation can be measured through both its oxygen consumption and its proton production. Thus combined respirometric±titrimetric data sets can be collected (Devisscher, 1997;Gernaey et al, 2001;Petersen et al, 2000). The most important advantage of a combination of respirometric and titrimetric measurements, however, is that two independent measurements are obtained simultaneously for the same process.…”

Section: Introductionmentioning

confidence: 99%

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Modeling aerobic carbon source degradation processes using titrimetric data and combined respirometric–titrimetric data: Experimental data and model structure

Gernaey

Petersen

Comeau

et al. 2002

Biotech & Bioengineering

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Experimental data are presented that resulted from aerobic batch degradation experiments in activated sludge with simple carbon sources (acetate and dextrose) as substrates. Data collection was done using combined respirometric-titrimetric measurements. The respirometer consists of an open aerated vessel and a closed non-aerated respiration chamber for monitoring the oxygen uptake rate related to substrate degradation. The respirometer is combined with a titrimetric unit that keeps the pH of the activated sludge sample at a constant value by addition of acid and/or base. The experimental data clearly showed that the activated sludge bacteria react with consumption or production of protons during aerobic degradation of the two carbon sources under study. Thus, the cumulative amount of added acid and/or base could serve as a complementary information source on the degradation processes. For acetate, protons were consumed during aerobic degradation, whereas for dextrose protons were produced. For both carbon sources, a linear relationship was found between the amount of carbon source added and the amount of protons consumed (in case of acetate: 0.38 meq/mmol) or produced (in case of dextrose: 1.33 meq/mmol) during substrate degradation. A model taking into account substrate uptake, CO(2) production, and NH(3) uptake for biomass growth is proposed to describe the aerobic degradation of a C(x)H(y)O(z)-type carbon source. Theoretical evaluation of this model for reference parameters showed that the proton effect due to aerobic substrate degradation is a function of the pH of the liquid phase. The proposed model could describe the experimental observations with both carbon sources.

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Section: Experiments With Carbon Sources (Acetate and Dextrose)mentioning

confidence: 92%

Section: Data Setmentioning

confidence: 99%

“…A detailed description of the interpretation of the respirometric data can be found in Gernaey et al (2001).…”

Section: Data Interpretationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modeling aerobic carbon source degradation processes using titrimetric data and combined respirometric–titrimetric data: Experimental data and model structure

Gernaey

Petersen

Comeau

et al. 2002

Biotech & Bioengineering

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“…The experimental methodology to monitor aerobic batch substrate degradation processes using combined respirometric±titrimetric measurements has already been described in detail by Gernaey et al (2001Gernaey et al ( , 2002. A typical data set obtained by adding 1.563 mmol acetate (100 mg COD) to the aeration vessel of the set-up at t = 0 during a short-term batch experiment with activated sludge is shown in Fig.…”

Section: Experimental Methodologymentioning

confidence: 99%

Modeling aerobic carbon source degradation processes using titrimetric data and combined respirometric–titrimetric data: Structural and practical identifiability

Gernaey

Petersen

Dochain

et al. 2002

Biotech & Bioengineering

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The structural and practical identifiability of a model for description of respirometric-titrimetric data derived from aerobic batch substrate degradation experiments of a C(x)H(y)O(z) carbon source with activated sludge was evaluated. The model processes needed to describe titrimetric data included substrate uptake, CO(2) production, and NH(3) uptake for biomass growth. The structural identifiability was studied using the Taylor series method and a recently proposed generalization method. It showed that combining respirometric and titrimetric data allows structural identifiability of one extra parameter combination, the biomass yield, Y(H), compared to estimation on separate data sets, on condition that the nitrogen fraction in biomass (i(XB)) is known. However, data from short-term batch substrate degradation experiments were not sufficiently informative to allow practical identification of all structurally identifiable parameters. Combining respirometry and titrimetry resulted in improvements of parameter confidence intervals compared to estimation on separate respirometric or titrimetric data sets. However, the level of the improvement seems to be substrate dependent: parameter confidence intervals improved considerably more for dextrose than for acetate degradation models. Noteworthy is the finding that the half-saturation substrate concentrations can be different depending on whether they are estimated from respirometric or titrimetric data. Moreover, this difference appears to be dependent on the carbon source considered: for dextrose, titrimetry-based K(S) values are higher than respirometry-based values while for acetate the opposite was found. It was hypothesized that this can be explained by the different point in cell metabolism where the proton production or consumption takes place, leading to a corresponding difference in timing between pH effect and oxygen consumption. Finally, the biomass yield Y(H) and the nitrogen content of the biomass i(XB) could be estimated from combined respirometric-titrimetric data obtained with addition of a known amount of carbon source. Y(H) can also be estimated from r(O) data when the initial substrate concentration S(S)(0) is known. The values found correspond to values reported in literature, but, interestingly, also seem able to reflect the occurrence of storage processes when pulses of acetate and dextrose are added.

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Wastewater: Growth Kinetics of Microorganisms

Pollard

2003

Encyclopedia of Environmental Microbiology

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Distinguishing Metabolism from Growth Directly Measure Bacterial Growth Kinetics Measuring Bacterial Growth in Activated Sludge Respiration Versus Growth Kinetics Biofilms In Situ Growth Kinetics of Single Species

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Activated sludge monitoring with combined respirometric–titrimetric measurements

Cited by 68 publications

References 40 publications

Modeling aerobic carbon source degradation processes using titrimetric data and combined respirometric–titrimetric data: Experimental data and model structure

Modeling aerobic carbon source degradation processes using titrimetric data and combined respirometric–titrimetric data: Experimental data and model structure

Modeling aerobic carbon source degradation processes using titrimetric data and combined respirometric–titrimetric data: Structural and practical identifiability

Wastewater: Growth Kinetics of Microorganisms

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