Activated phenotype of circulating neutrophils in familial Mediterranean fever

Manukyan, Gayane; Petřek, Martin; Kriegová, Eva; Ghazaryan, Karine A.; Fillerová, Regina; Boyajyan, Anna

doi:10.1016/j.imbio.2012.10.007

Cited by 23 publications

(25 citation statements)

References 40 publications

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“…FMF attacks are characterised by neutrophilia and neutrophil infiltration to affected tissues 9 10. For this reason, we initially investigated NETs release by peripheral blood PMNs during periods of disease attack and remission.…”

Section: Resultsmentioning

confidence: 99%

“…However, little is known regarding the triggers of disease attacks, as well as the contribution of PMNs to the initiation of the attacks 1 2 10. To date, studies that describe the cellular expression or circulating levels of IL-1β during inflammatory attack, are either controversial or missing 9 43 44.…”

Section: Discussionmentioning

confidence: 99%

“…However, the role of PMNs in the induction and regulation of IL-1β-mediated inflammation in FMF has not been yet elucidated 9 10. During the last decade, the release of neutrophil extracellular traps (NET) has been described as an effector mechanism of PMNs in many infectious and non-infectious inflammatory diseases 11 12.…”

Section: Introductionmentioning

confidence: 99%

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Neutrophil extracellular traps regulate IL-1β-mediated inflammation in familial Mediterranean fever

et al. 2014

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Neutrophil extracellular traps regulate IL-1β-mediated inflammation in familial Mediterranean fever

et al. 2014

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“…27 The hallmark of the disease is recurrent, self-limiting febrile episodes of serosal and synovial inflammation and During inflammatory episodes, high number of abnormally activated polymorphonuclear neutrophils and monocytes circulate and influx into the affected sites. 28,29 Neutrophils are responsible for endothelial dysfunction, oxidative stress, and over production of adhesion molecules. 30 Moreover, attack-free periods of FMF are characterized by subclinical inflammation.…”

Section: Discussionmentioning

confidence: 99%

Glutathione-S-Transferase Variants are not Associated With Increased Carotid Intima-Media Thickness in Turkish Familial Mediterranean Fever Patients

Gürbüz

Bağcı²,

Hüzmelí

et al. 2016

Arch Rheumatol

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Objectives: This study aims to evaluate the carotid intima-media thickness (CIMT) in patients diagnosed with familial Mediterranean fever (FMF) and investigate whether there is a relationship between glutathione-S-transferase (GST) gene polymorphisms and CIMT. Patients and methods: Sixty FMF patients (17 males, 43 females; mean age: 31.43±11.36 years; range 18 to 45 years) and 60 healthy controls (22 males, 38 females; mean age: 29.8±5.82 years; range 18 to 40 years) were enrolled in this study. Polymerase chain reaction-restriction fragment length polymorphism methods were carried out to assess GST polymorphisms. CIMT was measured by carotid ultrasonography. Biochemical parameters were also evaluated using biochemical methods. Results: Right and left CIMT of FMF patients were statistically significantly higher than that of control group (CIMT right p=0.001 and CIMT left: p=0.033). There was no significant association in terms of GST polymorphisms between FMF and control groups. No significant association was observed between GST polymorphisms and CIMT. Low density lipoprotein, erythrocyte sedimentation rate, and fibrinogen levels were significantly higher in the patient group (p<0.05). The difference between groups was not significant in terms of other biochemical parameters (p>0.05). Conclusion: Although no significant association was observed between GST polymorphisms and CIMT in FMF patients and controls, CIMT was statistically significantly higher in FMF patients compared to controls.

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“…All samples were treated with RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and maintained at -80˚C. cDNA synthesis and qPCR were performed as described previously (27). Briefly, cDNA synthesis was performed using a Transcriptor First Stand cDNA Synthesis kit (Roche Diagnostics) and stored at -20˚C prior to further use.…”

Section: Methodsmentioning

confidence: 99%

Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome

Martirosyan

Petřek

Kishore

et al. 2016

Experimental and Therapeutic Medicine

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Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by thrombosis and recurrent fetal loss, with the persistent presence of antiphospholipid antibodies (aPLs). aPLs exert their pathogenic effect via the overproduction of tissue factor and activation of complement and several cell types, including endothelial cells, platelets and notably monocytes. As a result, a hypercoagulable state develops leading to APS-associated obstetric complications and fetal loss. Despite being far from optimal, treatment of APS usually includes heparin and low dose aspirin. Recently, plasma exchange (PE) therapy was successfully used in patients with APS with obstetric complications who did not respond to the standard treatment. Therefore, the present study investigated the mechanism underlying PE action, and aimed to determine whether PE affects the functional activity of APS monocytes by examining the expression of 11 mRNA transcripts encoding cytokines, signaling molecules and transcription factors. Monocytes were collected prior to and following the PE treatment from women with APS who experienced recurrent pregnancy losses, as well as from healthy volunteers. Compared with control cells, APS monocytes showed deregulated expression of interleukin (IL)-1β, IL-6, IL-23, chemokine (C-C motif) ligand 2 (CCL2), C-X-C motif chemokine 10 (CXCL10), toll-like receptor 2, and signal transducer and activator of transcription 3. PE treatment resulted in increased IL-1β, IL-6, IL-23, CCL2, P2X7 and tumor necrosis factor-α mRNA transcripts in APS monocytes, restoring the mRNA expression levels to within normal ranges. Furthermore, PE therapy counterbalanced the expression levels of CCL2 and CXCL10, the levels of which are indicative of T helper cell 1/2 balance. The results of the present study indicate that the altered transcriptional profile in APS monocytes was restored by the immunomodulatory effect of plasmapheresis.

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Activated phenotype of circulating neutrophils in familial Mediterranean fever

Cited by 23 publications

References 40 publications

Neutrophil extracellular traps regulate IL-1β-mediated inflammation in familial Mediterranean fever

Neutrophil extracellular traps regulate IL-1β-mediated inflammation in familial Mediterranean fever

Glutathione-S-Transferase Variants are not Associated With Increased Carotid Intima-Media Thickness in Turkish Familial Mediterranean Fever Patients

Immunomodulatory effects of therapeutic plasma exchange on monocytes in antiphospholipid syndrome

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