Activated growth signaling pathway expression in Ewing sarcoma and clinical outcome

Abstract

In our series, p-mTOR and p27(KIP1) protein overexpression were independently associated with better survival.

Different thresholds of ZEB1 are required for Ras-mediated tumour initiation and metastasis

“…K-Ras mutation also drives sarcoma initiation 28 where ZEB1 is induced and Pten is likewise downregulated, as opposed to mutated [28][29][30][31][32] . In addition, as noted above, a ZEB1 high/PTEN low pattern also marks fibroblasts in the stroma of K-Ras mutant tumours, and such loss of Pten in the tumour stroma has been shown to be important for tumour progression 33 .…”

“…Indeed, it has been demonstrated recently that Pten can be secreted and taken up by adjacent cells 43 , raising the possibility that elimination of Pten in fibroblasts is necessary to remove a secondary source of secreted Pten that would otherwise be taken up by adjacent tumour cells. K-Ras mutation also drives sarcoma initiation 28 where ZEB1 is induced and Pten is likewise downregulated as opposed to mutated [28][29][30][31][32] . Thus, the ZEB1, PTEN pathway can be induced by K-Ras in mesenchymal cells in the absence of adjacent carcinoma cells.…”

Transcription Factors Sp1 and p73 Control the Expression of the Proapoptotic Protein NOXA in the Response of Testicular Embryonal Carcinoma Cells to Cisplatin

“…Sections were counterstained with hematoxylin and analyzed by light microscopy. For immunoreactivity, a previously reported 0 -3 semi-quantitative scoring system for both the intensity of stain and the percentage of positive cells (labeling frequency percentage) was used (27). For intensity of stain, each case was visually compared with both positive and negative controls.…”

Sequential Inductions of the ZEB1 Transcription Factor Caused by Mutation of Rb and Then Ras Proteins Are Required for Tumor Initiation and Progression

“…Ras also drives sarcoma formation, and ZEB1 expression is linked to invasion and metastasis in these tumors, where epithelial-to-mesenchymal transition would not appear to be a factor. Taken together, these findings raise the possibility that ZEB1 may have roles in tumor progression extending beyond the ZEB1/miR-200 loop thought to facilitate invasion and metastasis (21)(22)(23)(24)(25).…”