Activated Circulating Myeloid-Derived Suppressor Cells in Patients with Dilated Cardiomyopathy

Abstract

Background/Aims: Myeloid-derived suppressor cells (MDSCs) are increased in inflammatory and autoimmune disorders. This study aims to evaluate the significance of MDSCs in dilated cardiomyopathy (DCM) patients. Methods: In total, 42 newly hospitalized DCM patients and 39 healthy controls were enrolled in the study. The frequencies of circulating CD14⁺HLA-DR^-/low MDSCs were determined by flow cytometry. Then, the functional properties of MDSCs in suppressing T cell proliferation and interferon-gamma (IFN-γ) production were measured in a co-culture model. Then, mRNA expression levels of various important molecules in peripheral blood mononuclear cells were measured by real time polymerase chain reaction. Furthermore, correlation analyses between MDSC frequencies and cardiac function parameters were also performed. Results: The frequencies of circulating CD14⁺HLA-DR^-/low MDSCs were significantly elevated in DCM patients compared with healthy controls. It showed that MDSCs from DCM patients more effectively suppressed T cell proliferation and IFN-γ production compared with those from healthy controls, which was partially mediated by arginase-1 (Arg-1). In addition, the correlation analysis suggested that MDSC frequencies were negatively correlated with left ventricular ejection fraction (LVEF), while positively with N-terminal pro-brain natriuretic peptide (NT-proBNP) in patients with DCM. Conclusions: Circulating activated MDSCs might play significant immunomodulatory roles in the pathogenesis of DCM.

Circulating Long Non-coding RNA ENST00000507296 Is a Prognostic Indicator in Patients with Dilated Cardiomyopathy

“…Several studies discovered that circulating exosomal small RNAs would be significant diagnostic and prognostic biomarkers in the pathogenesis of diseases, such as multiple myeloma 31 and castration-resistant prostate cancer 32 . Recently, activated circulating myeloid-derived suppressor cells were reported to play important immunomodulatory roles in DCM 33 . The miRNAs miR-155, -636, -646, and -639 were found to have the potential to perform risk stratification in children with DCM 34 .…”

Blood immune cell biomarkers in lung cancer

“…It is noteworthy that in the age‐matched control group individual people showed a somewhat high amount of HLA‐DR low monocytes. Although the existence of an unnoticed tumor cannot be excluded, circulating MDSC are not tumor‐specific and have been described in several other pathological conditions, such as viral infections , various chronic infections or dilated cardiomyopathy . These reports suggest that HLA‐DR low monocytes might be part of a conserved response to different endogenous and exogenous stress signals, including inflammation .…”

Response Gene to Complement-32 Promotes the Imbalance of Treg/Th17 in Patients with Dilated Cardiomyopathy

“…The diagnosis of DCM was based on the revised criteria established by the 1995 World Health Organization ⁄International Society and Federation of Cardiology Task Forceon the Classification of Cardiomyopathy [19]. In this investigation, DCM was defined as systolic dysfunction [left ventricular ejection fraction (LVEF) < 45%] with ventricular dilation [left ventricular end-diastolic diameter (LVEDD) > 55 mm] in the absence of an apparent secondary cause of cardiomyopathy, such as coronary heart disease, hypertensive heart disease, or valvular heart disease [20]. The exclusion criteria included ischaemic cardiomyopathy, hypertrophic cardiomyopathy, hypertensive heart disease, a history of uncontrollable or untreated hypertension for at least a year before the documentation of LV Cellular Physiology and Biochemistry Cellular Physiology and Biochemistry dysfunction, and other secondary cardiomyopathies such as sarcoidosis and amyloidosis, the presence of significant coronary artery stenosis on angiography and non-ischaemic DCM secondary to valvular heart disease, systemic hypertension, cardiac surgery or acute myocarditis, excessive alcohol abuse, pregnancy, endocrine disease, active infectious disease or collagen disease, medical history of autoimmune disease [12,21].…”