The main effort of this study was to enhance the enzymatic hydrolysis yields of cotton and viscose waste fibers via fed batch enzymatic hydrolysis. To do so, enzymes fed into slurries at two steps in fed batch mode. Also, the batch mode with the same amount of enzymes was implemented in order to be compared with the fed batch mode. The results showed that the released sugar contents from fed batch hydrolysis of cotton and viscose waste fibers were 29.0 and 32.3 g/l, respectively, but batch process released 25.0 and 30.3 g/l from cotton and viscose waste fibers, respectively, which confirms the increasing effect of fed batch process on the hydrolysis of both waste fibers. In addition, the morphological studies and enzymatic hydrolysis yields from pretreated cotton and untreated viscose waste fibers showed that alkali pretreatment for viscose waste fibers is not necessary, but has significant effect on enzymatic hydrolysis of cotton waste fibers; also, the differences in microcrystalline structures of viscose and cotton waste fibers have resulted in more enzymatic hydrolysis and then fermentation yields of viscose waste fibers. The amount of ethanol produced from cotton and viscose waste fibers were 6.9 and 8.1g/l, respectively.