Actions of MDMA at glutamatergic neuromuscular junctions

Sparks, Garrett; Dasari, Sameera; Cooper, Robin L.

doi:10.1016/j.neures.2003.12.007

Cited by 11 publications

(5 citation statements)

References 33 publications

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“…A second source of glutamate is the glutamate already in the cytoplasm, within the nerve terminals, that may not have been immediately taken up across the membrane during synaptic activity. The NMJ on the opener muscle in crayfish is used here to compare the previous findings related to glutamate uptake and to build on studies that addressed actions of 5-HT in enhancing synaptic transmission (Dudel, 1965;Dixon and Atwood, 1989;Crider and Cooper, 2000;Sparks et al, 2004). The NMJs on this tonic muscle are relatively low in synaptic efficacy as compared to higher output phasic motor nerve terminals in the crayfish (Cooper et al, 1995a or even those of the larval Drosophila NMJs (Cooper et al, 1995b) presented herein.…”

Section: Introductionmentioning

confidence: 99%

The regulation and packaging of synaptic vesicles as related to recruitment within glutamatergic synapses

Cooper

2012

Neuroscience

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Section: Introductionmentioning

confidence: 99%

The regulation and packaging of synaptic vesicles as related to recruitment within glutamatergic synapses

Cooper

2012

Neuroscience

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“…Responses were recorded with a 1X LU head stage and an Axoclamp 2A amplifier. The recording techniques for intracellular (EJPs) measures have been previously described (Dasari and Cooper, 2004a,b; Sparks et al, 2004; Stewart et al, 1994). The compound amplitude of EJP elicited by Is and Ib motor nerve terminals in segment 3 of muscle m6 was monitored (Kurdyak et al, 1994; Ruffner et al, 1999).…”

Section: Methodsmentioning

confidence: 99%

Effects of inhibiting mTOR with rapamycin on behavior, development, neuromuscular physiology, and cardiac function in larval Drosophila

et al. 2019

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Rapamycin and other mTOR inhibitors are being heralded as possible treatments for many human ailments. It is currently being utilized clinically as an immunomodulator after transplantation procedures and as a treatment for certain forms of cancer, but it has numerous potential clinical indications. Some studies have shown profound effects on life cycle and muscle physiology, but these issues have not been addressed in an organism undergoing developmental processes. This paper fills this void by examining the effect of mTOR inhibition by rapamycin on several different qualities of larval Drosophila. Various dosages of the compound were fed to second instar larvae. These larvae were monitored for pupae formation to elucidate possible life cycle effects, and a delay to pupation was quantified. Behavioral deficits were documented in rapamycin-treated larvae. Electrophysiological measurements were taken to discern changes in muscle physiology and synaptic signaling (i.e. resting membrane potential, amplitude of excitatory post-synaptic potentials, synaptic facilitation). Pupation delay and effects on behavior that are likely due to synaptic alterations within the central nervous system were discovered in rapamycin-fed larvae. These results allow for several conclusions as to how mTOR inhibition by rapamycin affects a developing organism. This could eventually allow for a more informed decision when using rapamycin and other mTOR inhibitors to treat human diseases, especially in children and adolescents, to account for known side effects.

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“…The recording arrangement was the same as previously described (Stewart et al ., 1994; Dasari & Cooper, 2004; Sparks et al ., 2004). Intracellular recordings in muscles were made with 30–60 MΩ resistance, 3M KCl‐filled microelectrodes.…”

Section: Methodsmentioning

confidence: 99%

The pharmacological and physiological profile of glutamate receptors at the Drosophila larval neuromuscular junction

Bhatt

Cooper

2005

Physiological Entomology

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Abstract. Drosophila larval muscles are commonly used for developmental assessment in regard to various mutations of synaptically relevant molecules. In addition, the molecular sequence of the glutamate receptors on the muscle fibre have been described; however, the pharmacological profiles to known agonists and antagonists have yet to be reported. Here, the responses of N‐methyl‐d‐aspartic acid, α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole‐propionate (AMPA), l‐glutamate, kainate, quisqualic acid, NBQX, AP5 and DNQX are characterized with regard to synaptic transmission and direct effects on the muscle fibres. The muscle fibres depolarize to application of glutamate or quisqualate and the excitatory postsynaptic potential (EPSP) amplitudes are diminished. Kainate does not alter the muscle membrane potential but does reduce the EPSP amplitude. The known antagonists NBQX, AP5 and DNQX have no substantial effect on synaptic transmission at 1 mm, nor do they block the response of quisqualate. Kainate may be acting as a postsynaptic antagonist or via autoreceptors presynaptically to reduce evoked transmission.

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Actions of MDMA at glutamatergic neuromuscular junctions

Cited by 11 publications

References 33 publications

The regulation and packaging of synaptic vesicles as related to recruitment within glutamatergic synapses

The regulation and packaging of synaptic vesicles as related to recruitment within glutamatergic synapses

Effects of inhibiting mTOR with rapamycin on behavior, development, neuromuscular physiology, and cardiac function in larval Drosophila

The pharmacological and physiological profile of glutamate receptors at the Drosophila larval neuromuscular junction

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