Action spectra for the rescue of temperature-sensitive mutants of vesicular stomatitis virus by ultraviolet-irradiated virions at nonpermissive temperature

Deutsch, Victor; Muel, B.; Brun, Gilbert

doi:10.1016/0042-6822(77)90426-3

Cited by 10 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This process was especially obvious when a high MOI of highly inactivated virus was used. It has previously been demonstrated that VSV proteins from high MOIs of highly UV-irradiated virus can complement ts mutants (12,13,15). These findings support the Bratt and Hightower (4) hypothesis: a lethally irradiated virus might be able to provide a necessary function to a virion which is noninfectious due to defective protein packaging rather than a defective genome.…”

Section: Minutes Uvsupporting

confidence: 73%

“…Furthermore, Deutsch et al (15) found that only group II VSV mutants could be complemented by both a functional surviving gene and by a structural protein of UV-irradiated virus, similar to the El mutant of NDV described here. Group II mutants of VSV represent lesions in the NS gene (31,35).…”

Section: Minutes Uvsupporting

confidence: 72%

“…UV irradiation has successfully been used to order amber mutants of phage T4 (25) and RNA-negative (RNA-) ts mutants of Sindbis virus (17). Surviving genes of UV-irradiated VSV have been shown to complement group II and IV mutants (14,15).…”

mentioning

confidence: 99%

See 2 more Smart Citations

UV irradiation analysis of complementation between, and replication of, RNA-negative temperature-sensitive mutants of Newcastle disease virus

Peeples¹,

Bratt²

1982

J Virol

View full text Add to dashboard Cite

Random UV irradiation-induced lesions destroy the infectivity of Newcastle disease virus (NDV) by blocking downstream transcription from the single viral promoter. The nucleocapsid-associated polypeptides most likely to be involved in RNA synthesis are located at the extreme ends of the genome: NP and P are promoter proximal genes, and L is the most distal gene. We attempted to order the two temperature-sensitive (ts) RNA-negative (RNA-) mutant groups of NDV by determining the UV target sizes for the complementing abilities of mutants Al and El. After UV irradiation, El was unable to complement Al, a result compatible with the A mutation lying in the L gene. In contrast, after UV irradiation, Al was able to complement El for both virus production and viral protein synthesis, with a target size most consistent with the E mutation lying in the P gene. UVirradiated virus was unable to replicate as indicated by its absence in the yields of multiply infected cells, either as infectious virus or as particles with complementing activity. After irradiation, ts mutant BlAP, with a non-ts mutation affecting the electrophoretic mobility of the P protein, complemented El in a manner similar to Al, but it did not amplify the expression of AP in infected cells. This too is consistent with irradiated virus being unable to replicate despite the presence of the components needed for replication of El. At high UV doses, Al was able to complement El in a different, UV-resistant manner, probably by direct donation of input polypeptides. Multiplicity reactivation has previously been observed at high-multiplicity infection by UV-irradiated paramyxoviruses. In this case, virions which are noninfectious because they lack a protein component may be activated by a protein from irradiated virions. Peeples, L. L. Rasenas, and M. A. Bratt, submitted for publication). As yet, no definite as-965 on July 7, 2020 by guest

show abstract

Section: Minutes Uvsupporting

confidence: 73%

Section: Minutes Uvsupporting

confidence: 72%

See 1 more Smart Citation

UV irradiation analysis of complementation between, and replication of, RNA-negative temperature-sensitive mutants of Newcastle disease virus

Peeples¹,

Bratt²

1982

J Virol

View full text Add to dashboard Cite

show abstract

“…The agD and rgD mutants could be tentatively classified into complementation groups by a UY-inactivation-rescue procedure in which cells are infected with one mutant at low multiplicity in the presence of UYinactivated wild-type virus or another mutant. This technique was developed by Deutsch IDeutsch, 1975, 1976Deutsch et al, 1977) for analysis of the function of ts mutants of YSY. The conclusion from preliminary analysis was that the majority of the agO and rgD mutants were L-protein mutants.…”

Section: Piry Virus In Drosophilamentioning

confidence: 99%

Rhabdovirus Genetics

Pringle¹

1987

The Rhabdoviruses

View full text Add to dashboard Cite

There are five families of RNA viruses in which the negative strand is sequestered in the extracellular virion. Viruses of two of these families, the Rhabdoviridae and the Paramyxoviridae, have unitary linear genomes, whereas viruses of the other three families, Arenaviridae, Bunyaviridae, and Orthomyxoviridae, have segmented genomes comprising, respectively, two, three, and seven or eight subunits. The informational macromolecules that comprise the genomes of rhabdoviruses and paramyxoviruses are among the largest functional RNA molecules and are exceeded in size only by those of the plus-strand coronaviruses. Reanney (1982Reanney ( , 1984 has calculated that the upper size limit for any RNA virus genome cannot be much in excess of 17,600 nucleotides (mol. wt. =5.7 x 10 6 ) as a consequence of the low copying fidelity of RNA polymerases. The segmentation of the genomes of the other negative-strand viruses may be a consequence of such constraints on molecular size or a device for decoupling the transcription of !ndividual genes. Whatever the reason, the genetic properties of the segmented-genome viruses differ substantially from those of the unsegmented-genome viruses, because variation in the former is generated by reassortment of genome subunits as well as by mutation. Mutation is the sole mechanism of variation in unsegmented-genome viruses, since the intramolecular recombination observed with positive-

show abstract

“…However, DEUTSCI~ et al (29) reported that mutant t8082 had features in common with ts mutants belonging to group II. It had been observed that ts mutants of four of the five original groups could be complemented in chick embryo cells by UV-irradiated wild type virus (25,26,27,28). The rescue of mutants of groups I and V under these conditions could be attributed to reutilisation of structural proteins supplied by the irradiated virus.…”

Section: Inter-and Tntra-genic Complementationmentioning

confidence: 99%

The genetics of vesiculoviruses

Pringle

1982

Archives of Virology

View full text Add to dashboard Cite

Action spectra for the rescue of temperature-sensitive mutants of vesicular stomatitis virus by ultraviolet-irradiated virions at nonpermissive temperature

Cited by 10 publications

References 27 publications

UV irradiation analysis of complementation between, and replication of, RNA-negative temperature-sensitive mutants of Newcastle disease virus

UV irradiation analysis of complementation between, and replication of, RNA-negative temperature-sensitive mutants of Newcastle disease virus

Rhabdovirus Genetics

The genetics of vesiculoviruses

Contact Info

Product

Resources

About