SUMMARYComplete encystment of Acanthamoeba occurred in peptone-yeast extractglucose medium supplemented with 50 mwmagnesium chloride. There was an increase in the RNA, protein and DNA contents of the cells which coincided with an increase in the proportion of binucleate amoebae and commitment to encystation, suggesting that encystation could be a consequence of the inhibition of cell division when nutrient concentrations were limiting growth.