Action of the opioid agonist FK 33-824 on porcine small and large luteal cells from the mid-luteal phase: effect on progesterone, cAMP, cGMP and inositol phosphate release

Kamiński, Tadeusz; Siawrys, Gabriela; Okrasa, S; Przala, J

doi:10.1016/s0378-4320(99)00047-0

Cited by 15 publications

(16 citation statements)

References 33 publications

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“…These findings imply the existence of crosstalk between signalling systems induced by opioids and FSH or PRL. It is possible that points of contact of signalling systems stimulated by FSH and opioid peptides are represented by adenyl cyclase and phosphoinositide-specific phospholipase C. Both enzymes are involved in the action of FK 33-824 on porcine small and large luteal cells [24] and porcine theca cells (Kaminski et al, unpublished information). However, because the mechanism of opioid peptide action inside ovarian cells is still only superficially understood, it is premature to speculate on the mechanism of this crosstalk in detail.…”

Section: Discussionmentioning

confidence: 99%

The physiological role of ?-endorphin in porcine ovarian follicles

et al. 2000

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-β-Endorphin-like immunoreactivity (β-END-LI) was measured by radioimmunoassay in porcine ovarian follicular fluid (FF) from small, medium and large follicles throughout the oestrous cycle. The concentration of β-END-LI in FF from small follicles collected on days 1-5 of the cycle was at least tenfold higher than in the fluid from any other follicles independently from their size and the period of the cycle. The level of β-END-LI in small follicles on days 6-10 was drastically decreased. Subsequently, on days 11-16 its concentration was enhanced and reduced again in preovulatory period of the cycle. Concentrations of β-END-LI in FF from medium follicles were relatively equal throughout the cycle (days 6-21). No significant differences in β-END-LI levels were found between small, medium and large follicles from days 17-21. However, β-END-LI concentrations in medium follicles on days 11-13 and 14-16 were statistically lower than those in small follicles. Moreover, the effects of FSH, prolactin (PRL), progesterone (P 4 ), testosterone (T) and 17 β-oestradiol (E 2 ) on β-END-LI release by granulosa cells (GCs) from large follicles and, on the other hand, the effects of the opioid agonist FK 33-824 alone or in combination with FSH, PRL or naloxone (NAL) on follicular steroidogenesis were studied. FSH drastically increased β-END-LI output in a dose-dependent fashion. This stimulatory effect of the gonadotrophin was inhibited by the highest dose of P 4 (10 -5 M). The effect of PRL and the steroids added to the cultures on β-END-LI release was negligible. FSH-or PRL-induced P 4 secretion by GCs was essentially abolished by both FK 33-824 and NAL. However, androstenedione (A 4 ) and testosterone output by the cells was greatly potentiated by FK 33-824. In the presence of NAL, FSH or PRL, A 4 release stimulated by FK 33-824 was suppressed to the basal level. Secretion of E 2 was completely free from the influence of FK 33-824 or NAL; only oestrone (E 1 ) output was modulated by them in cultures where FSH or PRL was present. In conclusion, FSH appears to be the key regulator of β-END-LI secretion by porcine granulosa cells. Moreover, steroidogenesis in pig granulosa cells is modulated by opioid peptides acting both alone and by way of interaction with FSH or PRL.opioid peptides / β-endorphin / porcine granulosa cells / steroid secretion Reprod. Nutr. Dev. 40 (2000) 63-75 63

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Section: Discussionmentioning

confidence: 99%

The physiological role of ?-endorphin in porcine ovarian follicles

et al. 2000

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“…Luteal cells were isolated by the method described by Kaminski et al [25]. Dissected corpora lutea from ovaries on days 2-3, 10–12, and 14–16 were minced into small fragments and dispersed in F-12 medium containing bovine serum albumin fraction V (BSA; 1%) and antibiotics.…”

Section: Methodsmentioning

confidence: 99%

Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis

Maleszka

Smolińska

Nitkiewicz

et al. 2014

International Journal of Endocrinology

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Adiponectin is an adipose-secreted hormone that regulates energy homeostasis and is also involved in the control of the reproductive system. The goal of the present study was to investigate changes in adiponectin gene and protein expression in porcine ovarian structures during the oestrous cycle and to examine the effects of in vitro administration of adiponectin on basal and gonadotrophin- and/or insulin-induced secretion of ovarian steroid hormones. Both gene and protein expression of adiponectin were enhanced during the luteal phase of the cycle. Adiponectin affected basal secretion of progesterone by luteal cells, oestradiol by granulosa cells, and testosterone by theca interna cells. The gonadotrophin/insulin-induced release of progesterone from granulosa and theca interna cells and the release of oestradiol and androstenedione from theca cells was also modified by adiponectin. In conclusion, the presence of adiponectin mRNA and protein in the porcine ovary coupled with our previous results indicating adiponectin receptors expression suggest that adiponectin may locally affect ovarian functions. The changes in adiponectin expression throughout the oestrous cycle seem to be dependent on the hormonal status of pigs related to the stage of the oestrous cycle. The effect of adiponectin on ovarian steroidogenesis suggests that this adipokine influences reproductive functions in pigs.

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“…Moreover, the inhibition of PI hydrolysis suppressed steroid hormone secretion by porcine theca cells (Kaminski et al, 2003). The accumulation of IPs was also associated with an increase in P 4 production in luteal cells of primates and pigs (Ciereszko et al, 1995;Kaminski et al, 1999). It should be emphasised that, according to Yuan and Connor (1993), the activation of the PKC/[Ca 2+ ] i system can increase P 4 secretion by porcine luteal cells before the cells acquire luteolytic capacity.…”

Section: Discussionmentioning

confidence: 98%

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

Franczak

Kurowicka

Kowalik

et al. 2009

Acta Veterinaria Hungarica

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Oxytocin (OT) is involved in the regulation of steroid secretion by the corpus luteum (CL) in pigs, but OT signal transduction in the porcine CL has not been identified. In this study, the effects of OT on in vitro progesterone (P 4 ) secretion, phosphoinositide (PI) hydrolysis and intracellular mobilisation of Ca 2+ ([Ca 2+ ] i ) were investigated in porcine luteal cells during the early (days 3-5), mid-(days 8-10) and late luteal phases (days 12-14) of the oestrous cycle. Basal concentrations of P 4 and accumulation of inositol phosphates (IPs) were higher (P < 0.05) on days 3-5 and 8-10 of the oestrous cycle than on days 12-14. Basal [Ca 2+ ] i mobilisation did not differ among studied periods of the oestrous cycle. Oxytocin (10 -7 M) enhanced P 4 secretion and PI hydrolysis (P < 0.05) by luteal cells harvested on days 8-10 of the oestrous cycle. Moreover, OT started to increase mobilisation of [Ca 2+ ] i at the 15th (days 3-5 and 8-10) or 30th second (days 12-14) in porcine luteal cells. It was concluded that in pigs OT acts as a regulator of steroidogenesis, stimulating P 4 secretion in mature CL. This OT action may be mediated by changes in PI hydrolysis and [Ca 2+ ] i mobilisation.

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Action of the opioid agonist FK 33-824 on porcine small and large luteal cells from the mid-luteal phase: effect on progesterone, cAMP, cGMP and inositol phosphate release

Cited by 15 publications

References 33 publications

The physiological role of ?-endorphin in porcine ovarian follicles

The physiological role of ?-endorphin in porcine ovarian follicles

Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

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