Action of prostaglandins, endoperoxides, and thromboxanes on the lamb ductus arteriosus

Coceani, Flavio; Bishai, Isis; White, E.; Bodach, E.; Olley, P. M.

doi:10.1152/ajpheart.1978.234.2.h117

Cited by 24 publications

(16 citation statements)

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“…The procedure for cesarean delivery of lambs and isolation of the ductus arteriosus followed a published protocol (Coceani et al 1978). The ductus was freed of loose connective tissue, opened, and then cut perpendicularly to the main axis to yield one or two strips, depending on its length.…”

Section: Ge~zeral Paoceidegaementioning

confidence: 99%

Occurrence of endothelium-derived relaxing factor – nitric oxide in the lamb ductus arteriosus

Coceani¹,

Kelsey²,

Seidlitz³

1994

Can. J. Physiol. Pharmacol.

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To determine whether the ductus arteriosus can form endothelium-derived relaxing factor--nitric oxide, we used isolated ductal strips from near-term fetal lamb and examined their response to bradykinin (a nitric oxide stimulator), L-arginine (a nitric oxide precursor), and agents interfering with the synthesis (N omega-nitro-L-arginine) and action (methylene blue) of nitric oxide. Bradykinin relaxed the indomethacin-contracted ductus dose dependently from a threshold of about 10(-10) M, and peak relaxation was greater at high (176-210 mmHg; 1 mmHg = 133.3 Pa) than low (15-25 mmHg) PO2. Bradykinin relaxation was nearly completely or completely abolished in endothelium-denuded preparations and, in its place, there was often a small contraction. Pretreatment with nitric oxide inhibitors also prevented, in part (methylene blue, 1 microM) or in full (N omega-nitro-L-arginine, 100 microM), the relaxant effect of bradykinin. Paradoxically, L-arginine (10 microM) had an inhibiting rather than an enhancing effect on the bradykinin relaxation. N omega-Nitro-L-arginine (100 microM) and methylene blue (1-100 microM) contracted by themselves the untreated ductus, and their action persisted after removal of the endothelium. These findings indicate the presence in the ductus arteriosus of a nitric oxide based relaxing mechanism, which may supplement prostaglandin E2 in keeping the vessel patent in the fetus. This mechanism may, on one hand, afford protection against nonsteroidal anti-inflammatory drugs in utero and may, on the other hand, complicate the management of prematures with persistent ductus and account for failures of the indomethacin therapy.

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Section: Ge~zeral Paoceidegaementioning

confidence: 99%

Occurrence of endothelium-derived relaxing factor – nitric oxide in the lamb ductus arteriosus

Coceani¹,

Kelsey²,

Seidlitz³

1994

Can. J. Physiol. Pharmacol.

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“…An interesting issue emerging from this work concerns the differences between the ductus venosus and the ductus arteriosus with regard to the organization of the prostaglandin system and the response to oxygen (Coceani et al, 1978;Coceani and Olley, 1982). Unlike the ductus arteriosus, the ductus venosus contracts weakly to oxygen, and responses are normally not sustained.…”

Section: Ductus Venous Sphincter From Fetal and Neonatal (1 -Dayoldmentioning

confidence: 99%

The response of the lamb ductus venosus to prostaglandins and inhibitors of prostaglandin and thromboxane synthesis.

Adeagbo¹,

Coceani²,

Olley³

1982

Circulation Research

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SUMMARY. Rings of the umbilical end of the ductus venosus (the so-called sphincter) from nearterm fetal and newborn (1-3 days of age) lambs were studied in vitro at low (17-31 mm Hg) and high (504-705 mm Hg) P02. Tissues developed a modest contraction upon exposure to a high P02; however, this contraction generally was not sustained and tended to be greater in the fetus than in the newborn. In contrast, excess potassium (55 min) produced a sustained contraction whose magnitude was greater in the newborn. The cyclooxygenase inhibitor, indomethacin, contracted both the fetal and the neonatal ductus venosus; however, responses in the newborn were often not maintained. Furthermore, indomethacin enhanced the potassium response in the fetus, whereas it had no significant effect in the newborn. The thromboxane synthesis inhibitor, compound OKY-1581, was ineffective in either age group. Prostaglandin (PG) E2 and PGI 2 relaxed the ductus venosus, the former being slightly more potent. Conversely,stable PG endoperoxide analogs (9a,lla-epoxymethano and 9a,lla-methano-epoxy compounds) and PGF20 were contractile agents. Endoperoxide analogs were more effective in the newborn than the fetus and their action consistently exceeded that of PGF20. We conclude that the ductus venosus sphincter is endowed with functional muscle cells. These cells are under the influence of a relaxing product of the cyclooxygenase reaction which may be identified with either PGE2 or PGI2. This prostaglandin-mediated relaxing mechanism may contribute to prenatal patency of the vessel. Postnatal closure of the ductus venosus is unlikely to be a direct effect of oxygen on the sphincter muscle. The prostaglandin endoperoxides, however, are well suited for playing a role in the latter process. (Circ Res 51: 580-586, 1982)

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“…As hatching progresses to the EP stage, the DA contractile response to O 2 increases significantly (Belanger et al, 2008;Copeland and Dzialowski, 2009) and the vessel begins to constrict (Belanger et al, 2008). This developmental responsive pattern is similar to mammalian species with increases in DA O 2 -sensitivity occurring at the end of gestation and at birth in mammals (Coceani et al, 1978(Coceani et al, , 1979Kajino et al, 2001).…”

Section: Introductionmentioning

confidence: 61%

In vitro oxygen exposure promotes maturation of the oxygen sensitive contraction in pre-term chicken ductus arteriosus

Greyner

Dzialowski

2015

Comparative Biochemistry and Physiology Part A: Molecular &

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The ductus arteriosus (DA) are O2-sensitive, embryonic blood vessels that serve as a right-to-left shunt in developing avian embryos. Prior to internal pipping, the chicken DA produces a weak O2-induced contraction. During hatching, the O2-sensitivity of the avian DA vessels increases significantly. To see if we could accelerate the maturation of chicken DA O2-sensitivity, we exposed the vessel in vitro to elevated O2 (25 kPa) for 3-h prior to internal pipping on day 19 of incubation. The DA initially responded to increasing O2 with a weak contraction (0.15±0.04 N/m) that significantly increased in strength (0.63±0.06 N/m) during 3-h 25 kPa O2 exposure. A tonic influence of nitric oxide, not present at low O2, appeared during the 3-h 25 kPa O2 exposure. The long-term O2-induced contraction was mediated by both L-type Ca(2+) channels and internal Ca(2+) stores. The Rho-kinase pathway inhibitors Y-27632 and fasudil produced significant relaxation, suggesting a role for Ca(2+) sensitization in the contractile response to the 3h of elevated O2. While the day 19 DA initially exhibited an immature contractile response to O2, maturation of the pathways regulating O2-induced contraction was accelerated by exposure to 25 kPa O2, producing contractions similar in magnitude to those found during the final stage of hatching. This suggests that maturation of O2-sensitivity may be accelerated in vivo by increasing arterial O2 levels.

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Action of prostaglandins, endoperoxides, and thromboxanes on the lamb ductus arteriosus

Cited by 24 publications

References 0 publications

Occurrence of endothelium-derived relaxing factor – nitric oxide in the lamb ductus arteriosus

Occurrence of endothelium-derived relaxing factor – nitric oxide in the lamb ductus arteriosus

The response of the lamb ductus venosus to prostaglandins and inhibitors of prostaglandin and thromboxane synthesis.

In vitro oxygen exposure promotes maturation of the oxygen sensitive contraction in pre-term chicken ductus arteriosus

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