Action of low-power laser irradiation on the proliferation of human gingival fibroblasts in vitro

Abstract

The low level power laser has hbeen used in dental treatmens aiming to improve tissue healing . An in vitro study was performed to analyse the laser influence on gengival fibroblast. A human gingival fibroblast culture ( LMF ) was produced in DME mediun with 1 O%bovine fetal serum (BFS) cells( LMF) were allocated in Petri plates and cultured in different SFB concentrations ( 0% , 5% e 1O%).After 48 hours the plates were divided in 9 groups : 3 control : 3 irradiated by 635nm laser ;and 3 irradiated by 780 nm laser . The cultured cells received 4 applications, in 1 2 hours intervals , with energy dosage of 2 joules for each plate by means of a punctual techniqueThe growth curves showed that the growth levels were lower in low BFS concentrations . The irradiadation with laser accelerated the growth rate in all groups.Additionally , the number of cells developed in low BFS concentration ( 5% ) and irradiated was similar to the number of control cells developed in ideal conditions (1 0% BFS )There was no statistically significant differences between the effects of the two types of laser studied.

Low-Level Laser Therapy in the Prevention and Treatment of Chemotherapy-Induced Oral Mucositis in Young Patients

“…Laser therapy enhances microcirculation, 17-20 improves lymphatic drainage, 21-23 promotes pain relief, [24][25][26][27][28][29] and increases proliferation and mobility of epithelial cells. 16,[30][31][32] There are also significant increases in fibroblast production and activity, [33][34][35][36][37][38] which accelerate collagen synthesis. [39][40][41][42] Our improved understanding of the pathophysiology of OM has made possible the introduction of new approaches to treat the manifestations of stomatotoxicity.…”

Photobiomodulation of human gingival fibroblasts with diode laser - A systematic review

“…in 2006 were performed on the same primary cell line harvested from a 24 years old female. [ 9 10 11 ]…”

“…with 10% FBS except Almeida-Lopes et al . [ 9 10 ] and Azevedo et al . [ 11 ] These studies evaluated the effect under stressed conditions, i.e.…”

“…[ 11 ] exposed the gingival fibroblasts to 5% serum concentration 2 days before irradiation whereas the duration of exposure was not mentioned by Almeida-Lopes et al . [ 9 10 ] The allocation of cells to experiment and control groups was not concealed to the observer in any study. The laser device was not calibrated before use in any study and four out of nine studies reported the use of power meter to check laser output during the actual experiment [ Table 2 ].…”

“…The diode lasers used in the range of 600–700 nm had the following wavelength – 635 nm, 660 nm, 670 nm, 685 nm, and 692 nm. Almeida-Lopes et al .,[ 9 10 ] Azevedo et al . [ 11 ] and Saygun et al .…”