Abstract:1974. Action of light on Micrococc~ts roseus. Can. J.Microbiol. 20: 1015-1021. Micrococc~ts roseus is killed by exposure to a continuous spectrum of white light (3000 ft-c (32 292 Im/m2)) in the presence of air and the dye, toluidine blue. Comparison of rates of photodynamic killing for log and stationary phase cells and for two pigmentation mutants indicated that photosensitivity could not be correlated with amount of carotenoid or with carotenoid-chromophore length. Moreover, cells grown in medium containing… Show more
“…Procedures similar to those used here were used to demonstrate repair of photodynamic damage in Escherichia coli (Das et al, 1974). However, we were not able to substantiate the suggestion of Schwartzel and Cooney (1974b) that M . roseus has a repair mechanism.…”
Section: Resultscontrasting
confidence: 94%
“…The absorption spectrum of pigments from the wild type (Fig. 2) are typical of a mixture of canthaxanthin, echinenone, 4-hydroxyechinenone, and other pigments with a /I-carotene chromophore previously identified (Cooney et a!., 1966;Schwartzel and Cooney, 1970, 1972, 1974b. Two mutants, labeled W H and 10.5, respectively, appear to lack colored carotenoids.…”
Section: Resultsmentioning
confidence: 76%
“…The bacterium Micrococcus roseus is killed when cells are exposed to white light (3000 ft-c, 47 J/s/mz) in the presence of oxygen and a photosensitizing dye. The phenomenon, known as lethal photodynamic action (PDA), is not observed if the exogenous photosensitizing dye is omitted, even when cells are exposed for as long as 9 h to light of an intensity of 22,000 ft-c (348 J/s/m2) (Schwartzel and Cooney, 1974b). This led to the suggestions that M .…”
Section: Introductionmentioning
confidence: 99%
“…roseus synthesizes a number of carotenoids (Cooney, et al, 1966;Schwartzel and Cooney, 1970, 1972, 1974a. Cells grown in the presence of diphenylamine (DPA) do not contain significant quantities of colored carotenoids but they accumulate colorless carotenoid precursors (Schwartzel and Cooney, 1974b fore, a second objective of the present work was to obtain mutants of M . roseus which lack colored carotenoids and to compare their sensitivity to PDA with that of the pigmented parent organism in order to determine if carotenoids protect the organism against lethal PDA.…”
Abstract. Previous work showed that the bacterium Micrococcus roseus is killed by photodynamic action when an exogenous photosensitizer is present, but when a photosensitizer is not added the organism survives long exposure to high intensity (22,000ft‐c, 348 J/s/m2) white light. Experiments designed to demonstrate the presence of a mechanism to repair damage caused by photodynamic action failed to reveal such a mechanism. However, the organism was killed by light of a very high intensity (32,000ft‐c, 506 J/s/m2) in the absence of added photosensitizer, indicating that cells have an effective endogenous photosensitizer(s). Two carotenoid‐deficient mutants were killed via photodynamic action more rapidly than the fully pigmented wild‐type in the presence or absence of an exogenous photosensitizer. Thus, resistance of M. roseus to photodynamic action is not due to a repair mechanism, nor to lack of an effective endogenous photosensitizer, but to the protective action of carotenoid pigments.
“…Procedures similar to those used here were used to demonstrate repair of photodynamic damage in Escherichia coli (Das et al, 1974). However, we were not able to substantiate the suggestion of Schwartzel and Cooney (1974b) that M . roseus has a repair mechanism.…”
Section: Resultscontrasting
confidence: 94%
“…The absorption spectrum of pigments from the wild type (Fig. 2) are typical of a mixture of canthaxanthin, echinenone, 4-hydroxyechinenone, and other pigments with a /I-carotene chromophore previously identified (Cooney et a!., 1966;Schwartzel and Cooney, 1970, 1972, 1974b. Two mutants, labeled W H and 10.5, respectively, appear to lack colored carotenoids.…”
Section: Resultsmentioning
confidence: 76%
“…The bacterium Micrococcus roseus is killed when cells are exposed to white light (3000 ft-c, 47 J/s/mz) in the presence of oxygen and a photosensitizing dye. The phenomenon, known as lethal photodynamic action (PDA), is not observed if the exogenous photosensitizing dye is omitted, even when cells are exposed for as long as 9 h to light of an intensity of 22,000 ft-c (348 J/s/m2) (Schwartzel and Cooney, 1974b). This led to the suggestions that M .…”
Section: Introductionmentioning
confidence: 99%
“…roseus synthesizes a number of carotenoids (Cooney, et al, 1966;Schwartzel and Cooney, 1970, 1972, 1974a. Cells grown in the presence of diphenylamine (DPA) do not contain significant quantities of colored carotenoids but they accumulate colorless carotenoid precursors (Schwartzel and Cooney, 1974b fore, a second objective of the present work was to obtain mutants of M . roseus which lack colored carotenoids and to compare their sensitivity to PDA with that of the pigmented parent organism in order to determine if carotenoids protect the organism against lethal PDA.…”
Abstract. Previous work showed that the bacterium Micrococcus roseus is killed by photodynamic action when an exogenous photosensitizer is present, but when a photosensitizer is not added the organism survives long exposure to high intensity (22,000ft‐c, 348 J/s/m2) white light. Experiments designed to demonstrate the presence of a mechanism to repair damage caused by photodynamic action failed to reveal such a mechanism. However, the organism was killed by light of a very high intensity (32,000ft‐c, 506 J/s/m2) in the absence of added photosensitizer, indicating that cells have an effective endogenous photosensitizer(s). Two carotenoid‐deficient mutants were killed via photodynamic action more rapidly than the fully pigmented wild‐type in the presence or absence of an exogenous photosensitizer. Thus, resistance of M. roseus to photodynamic action is not due to a repair mechanism, nor to lack of an effective endogenous photosensitizer, but to the protective action of carotenoid pigments.
“…Cooney et al (165), Ungers and Cooney (826), and Schwartzel and Cooney (710) reported canthaxanthin to be the major pigment in Micrococcus roseus. Canthaxanthin has its widest distribution in marine organisms.…”
Section: Canthaxanthin For Yolk Pigmentationmentioning
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