Action of L-alanine on the fluxes of inorganic ions across the intestinal epithelium of the Greek tortoise

Gilles-Baillien, M.; Schoffeniels, E.

doi:10.1016/0024-3205(67)90019-7

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…However, if Na was replaced by tris(hydroxymethyl)aminomethane (Tris) (constant C1) or if C1 was replaced by SO 4 (constant Na), no effect on alanine flux was observed. Finally, Gilles-Baillien and Schoffeniels (9) found that alanine added to the mucosal solution had no clear effect on net Na flux but markedly altered net C1 flux. On the basis of these results, they argue for a completely different mechanism of amino acid transport in turtle intestine than that proposed for rabbit intestine (3,18).…”

Section: Discussionmentioning

confidence: 95%

“…METHODS Before measuring the unidirectional efflux of alanine from the cell to the serosal solution, we felt it necessary to examine some general aspects of alanine transport in turtle intestine since the present study might be of more general interest if the process were similar to that observed in mammalian intestine. Certain features of the transport process, particularly with respect to Na dependence, required further study in view of observations of Gilles-Baillien and Schoffeniels (8)(9)(10)(11) suggesting differences between turtle and mammalian intestine. Thus several types of experiments were carried out as described below.…”

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confidence: 99%

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Alanine Efflux across the Serosal Border of Turtle Intestine

Hajjar

Khuri

Curran

1972

The Journal of General Physiology

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The exit of alanine across the serosal border of the epithelial cells of turtle intestine was measured by direct and indirect techniques. A decrease or an increase in cell Na did not affect the amino acid flux from cell to serosal solution. Cells loaded with Na and alanine did not exhibit any extrusion of alanine when their serosal membranes were exposed to an Na-free medium containing alanine. However, substantial amino acid extrusion was observed across the mucosal cell border under similar conditions. Although alanine flux across the serosal membrane appeared to be Na-independent, it showed a tendency toward saturation as cellular alanine concentration was elevated. The results are consistent with the postulate that the serosal and mucosal membranes of intestinal cells are asymmetrical with respect to amino acid transport mechanisms. The serosal membrane appears to have an Naindependent carrier-mediated mechanism responsible for alanine transport while transport across the mucosal border involves an Na-dependent process.

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Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

Alanine Efflux across the Serosal Border of Turtle Intestine

Hajjar

Khuri

Curran

1972

The Journal of General Physiology

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“…D. A. HUDSON AND R. J. LEVIN across the intestinal wall (Schachter & Britten, 1961;Barry, Dikstein, Matthews, Smyth & Wright, 1964;Schultz & Zalusky, 1965; Barry, 1967). The exact nature of the electrogenic mechanism generating these transfer potentials is still in doubt in rat (Barry, Smyth & Wright, 1965) and tortoise intestine (Gilles-Baillien & Schoffeniels, 1967), but studies with rabbit ileum have shown that the active transfer of hexoses and amino acids is linked with an increased transfer of sodium ions that generates the transfer p.d. (Schultz & Zalusky, 1965).…”

Section: Introductionmentioning

confidence: 99%

The ontogeny of electrical activity associated with absorption of solutes across the developing small intestine of the chick (Gallus domesticus)

Hudson¹,

Levin²

1968

The Journal of Physiology

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1. Electrical potentials across the developing intestine of the chick were measured in vitro 3-4 days before and up to 32 days after hatching.2. Embryonic intestine had a very high endogenous p.d. (serosa positive to mucosa) which decreased up to hatching. This decrease continued during intestinal maturation, reaching a basal level several days after hatching.3. Transfer potential differences were caused only by the actively transferred hexoses both in embryonic and post-embryonic intestine. These potentials decreased concomitantly with the decrease in the endogenous p.d. Kinetic analysis showed that the ;apparent K(m)' of glucose for the glucose transfer p.d. generating mechanism did not change over the period of hatching.4. Both the embryonic and post-embryonic transmural p.d. either in the presence or absence of metabolizable hexose was dependent upon aerobic metabolism.5. The transmural p.d. of hatched chick small intestine (with and without hexoses) was linearly related to log(10)[Na(+)] of the mucosal fluid. The magnitude of the glucose transfer p.d. both in embryonic and post-embryonic small intestine, however, was not lowered by decreasing the mucosal [Na(+)].6. Amino acid transfer potentials were of small magnitude at all stages of development.

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Electrical Activity of the Intestine

Barry¹,

Eggenton²

1974

Intestinal Absorption

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Action of L-alanine on the fluxes of inorganic ions across the intestinal epithelium of the Greek tortoise

Cited by 8 publications

References 9 publications

Alanine Efflux across the Serosal Border of Turtle Intestine

Alanine Efflux across the Serosal Border of Turtle Intestine

The ontogeny of electrical activity associated with absorption of solutes across the developing small intestine of the chick (Gallus domesticus)

Electrical Activity of the Intestine

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