Action of Insulin on Thyroid Protein Synthesis in Dogs

A, Brisson-Lougarre; Blum, C; Ph, De Nayer

doi:10.1055/s-0028-1093400

Cited by 6 publications

(4 citation statements)

References 2 publications

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“…Because of its low K,, the lo-'' M optima receptor probably represents a genuine insulin receptor. Specific binding of insulin to nuclei was reported by Goldfine in 1977 [I] and has since been confirmed by several other laboratories [2,26]. The structure of the nuclear receptor has been recently published [27].…”

Section: Discussionmentioning

confidence: 84%

Effect of physiological concentrations of insulin and antidiabetic drugs on RNA release from isolated liver nuclei

Schumm

Webb

1983

J of Cellular Biochemistry

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The addition of 10-"M insulin to a cell-free system from rat liver promotes the release of messengerlike RNA from isolated prelabeled nuclei. The stimulation was similar whether the nuclei were preincubated with insulin, or if insulin was added directly to the cell-free system with or without a protease inhibitor. Dot blot hybridization using cloned cDNA for a2,-globulin mRNA showed that this was one of the messages whose release was enhanced by insulin. Nuclei isolated from rats treated with either of the antidiabetics tolbutamide or tolazamide showed no increase in RNA release in the presence of insulin over the concentration range 10-5-10-14 M . Furthermore, these nuclei did not release detectable levels of aZuglobulin mRNA.Key words: insulin, anti-diabetic drugs, tolazamide, tolbutamide, RNA transport, olz,-globulin mRNAInsulin is known to produce both immediate and late metabolic effects. The latter may be associated with the internalization of the hormone and its binding to high-affinity sites on the nuclear membrane [1,2]. Insulin binding to the nuclear membrane has been shown to stimulate the nuclear pore ATPase [3] and mRNA, but not rRNA, efflux from the nucleus to the cytoplasm in a cell-free system [4]. Since these latter experiments required insulin concentrations greater than physiological, it was of interest to determine the cause of the high insulin requirement and, if possible, to modify the system to respond to lower insulin levels.Among the specific long-term effects of insulin is the enhancement of the concentration of mRNA for albumin [5], az,-globulin [6], and tyrosine amino transferase [ 7 ] . If nuclei incubated in the cell-free system, which has previously been shown to release functional mRNA [8], are responding to insulin as do nuclei in vivo, it should be possible to detect an increased amount of these mRNAs in the RNAs released in vitro in the presence of insulin. The availability of a cloned cDNA to a2"-globulin mRNA [9] makes such a study feasible at this time.One of the acute in vivo effects of the oral antidiabetic drugs tolbutamide and tolazamide is to increase the levels of circulating insulin. These drugs have been

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Section: Discussionmentioning

confidence: 84%

Effect of physiological concentrations of insulin and antidiabetic drugs on RNA release from isolated liver nuclei

Schumm

Webb

1983

J of Cellular Biochemistry

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“…In addition, specific nuclear binding sites have been detected in thyroid nuclei [35,36]. The major site of insulin binding to the nucleus is the nuclear envelope [37-391.…”

Section: Binding Sites For Insulin On Nuclear Envelopes and Plasma Mementioning

confidence: 99%

Insulin binding sites on the nuclear envelope: potential relationship to mRNA metabolism

Goldfine

Purrello

Clawson

et al. 1982

J of Cellular Biochemistry

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Insulin regulates the growth and metabolism of most tissues. The hormonal potency of insulin results, to a large extent, from its ability to regulate target cells at a variety of subcellular sites. For many years, the effects of insulin on membrane transport, enzyme activity, and protein synthesis have been studied extensively. Less attention, however, was given to how insulin regulates nuclear functions. Recently the presence of specific binding sites for insulin on nuclei and nuclear envelopes have been documented and characterized. These binding sites have biochemical characteristics that are different from insulin binding sites on the plasma membrane. Moreover, direct in vitro effects of insulin on messenger RNA (mRNA) metabolism have recently been reported. These effects include the stimulation of mRNA efflux from intact nuclei, and stimulation of nucleoside triphosphatase activity (NTPase), the enzyme that regulates mRNA efflux. Thus, significant insight is now being gained concerning the action of insulin on the cell nucleus.

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“…In man insulin administration is reported to cause an increase of plasma T4 because of an increase of hepatic release of this hormone (Blum et al, 1973); also free thyroxine (FT4) is reported to increase (Negoescu et al, 1977). Brisson and Lougarre (1978) report that insulin causes an increase of protein synthesis, with a direct action on the thyroid cells both in vivo and in vitro. In the pig, Sho et al (1984) report stimulation of functional activity, with strong increase of iodine uptake and of organification.…”

Section: Introductionmentioning

confidence: 99%

The effects of insulin on TSH secretion and the morphology and physiology of the thyroid in the lizard Podarcis s. sicula

et al. 1996

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Insulin was administered to Podarcis sicula in winter, when the thyroid gland is inhibited. The activity of the thyroid increased, plasma concentrations of thyroid hormones and hepatic 5'-monodeiodinase activity (MDA) increased, and thyroid stimulating hormone (TSH) concentrations fell to undetectable values. This result confirms the influence of insulin on the activity of the thyroid gland in the lizard species studied. The mechanisms are still unclear, although there is evidence which leads us to believe that insulin is directly responsible for thyroid activation.

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Action of Insulin on Thyroid Protein Synthesis in Dogs

Cited by 6 publications

References 2 publications

Effect of physiological concentrations of insulin and antidiabetic drugs on RNA release from isolated liver nuclei

Effect of physiological concentrations of insulin and antidiabetic drugs on RNA release from isolated liver nuclei

Insulin binding sites on the nuclear envelope: potential relationship to mRNA metabolism

The effects of insulin on TSH secretion and the morphology and physiology of the thyroid in the lizard Podarcis s. sicula

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