Action of exonuclease III on γ-irradiated DNA

Bopp, Annemarie; Carpy, Serge; Burkart, Brigitte; Hagen, U.

doi:10.1016/0005-2787(73)90313-4

Cited by 34 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA from E. coli appears to contain some 3'-phosphoryl termini (52), but these may have been created during isolation (53). DNA having 3'-phosphoryl termini could also be produced by y-ray irradiation (54). The 3'-phosphoryl groups in DNA are known to interfere with a number of enzymes and, moreover, such DNAs are not substrates for enzymes such as DNA polymerase, polynucleotide ligase, and many exonucleases (55-59).…”

Section: Discussionmentioning

confidence: 99%

Polynucleotide Kinase

Kleppe¹

1979

Advances in Enzymology - And Related Areas of Molecular Biology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Polynucleotide Kinase

Kleppe¹

1979

Advances in Enzymology - And Related Areas of Molecular Biology

View full text Add to dashboard Cite

“…The 3 -hydroxyl ends of the DNA sugar-phosphate backbone, which are a likely outcome of SSB formation [70], are labelled with brominated deoxyuridine triphosphate (BrdUTP) nucleotides in a reaction catalysed by the terminal deoxynucleotidyl transferase (TdT) enzyme. This is followed by an incubation with an commercially available anti-BrdU mouse monoclonal antibody linked to horseradish peroxidase (HRP) that then catalyses the oxidation of species such as 3 ,3 ,5 ,5 -tetramethylbenzidine (TMB) to give a coloured product, which then can be detected spectrophotometrically.…”

Section: Vuv Induced Damage In Dnamentioning

confidence: 99%

Evaluating experimental molecular physics studies of radiation damage in DNA*

Śmiałek

2016

Eur. Phys. J. D

View full text Add to dashboard Cite

Abstract. The field of Atomic and Molecular Physics (AMP) is a mature field exploring the spectroscopy, excitation, ionisation of atoms and molecules in all three phases. Understanding of the spectroscopy and collisional dynamics of AMP has been fundamental to the development and application of quantum mechanics and is applied across a broad range of disparate disciplines including atmospheric sciences, astrochemistry, combustion and environmental science, and in central to core technologies such as semiconductor fabrications, nanotechnology and plasma processing. In recent years the molecular physics also started significantly contributing to the area of the radiation damage at molecular level and thus cancer therapy improvement through both experimental and theoretical advances, developing new damage measurement and analysis techniques. It is therefore worth to summarise and highlight the most prominent findings from the AMP community that contribute towards better understanding of the fundamental processes in biologically-relevant systems as well as to comment on the experimental challenges that were met for more complex investigation targets.

show abstract

“…Henner et al (9) suggest that the 3 0 terminus may contain two kinds of structures, i.e., the phosphoryl or phosphoglycolate group. However, Bopp et al (10) found that about 40% of the 3 0 termini were hydroxyl groups. In general, we find Henner's analysis is more acceptable (11), and we conclude that the phosphomonoester is the main structure of the 5 0 or 3 0 terminus of DNA strand breaks.…”

Section: Introductionmentioning

confidence: 99%

Detection of DNA Strand Breaks in Gamma-Irradiated Lymphocytes Using Surface Plasmon Resonance

et al. 2013

View full text Add to dashboard Cite

Surface plasmon resonance (SPR) is a sensitive, rapid, simple and low cost method for detection of biological molecules. In this study, SPR technology with alkaline phosphatase as a probe was utilized to measure DNA strand breaks induced by (60)Co gamma rays. The doses were from 0.01-10 Gy with a dose rate of 0.1 Gy/min. The results demonstrate that the SPR technology can be used to estimate strand breaks of calf thymus DNA. SPR signals of the calf thymus DNA samples increased with increasing gamma ray doses and the relationship of y = sqrt (3297x + 582.6) (r = 0.99) between the SPR signal and gamma dose was obtained. Estimation of DNA strand breaks in irradiated lymphocytes by SPR also demonstrated an increase in SPR signal with increasing dose and the exponential relationship of y = 169.43 × (1 - exp(-0.89x)) (r = 0.93) was obtained. The initial yield of the SPR signal is about 150.79 mdeg · Gy(-1) and compared to the sensitivity of 0.05 Gy achieved by the neutral single cell gel electrophoresis (SCGE), the SPR-based assay of DNA strand breaks was found to be more sensitive (0.02 Gy). We therefore propose that SPR technology with alkaline phosphatase as the probe is a sensitive, simple and quick method for detection of DNA strand breaks in gamma-irradiated lymphocytes.

show abstract

Action of exonuclease III on γ-irradiated DNA

Cited by 34 publications

References 14 publications

Polynucleotide Kinase

Polynucleotide Kinase

Evaluating experimental molecular physics studies of radiation damage in DNA*

Detection of DNA Strand Breaks in Gamma-Irradiated Lymphocytes Using Surface Plasmon Resonance

Contact Info

Product

Resources

About