Action of crude Radix Aconiti Lateralis (Fuzi) and its processed products on splenic lymphocytes growth investigated by microcalorimetry

Liu, Tiantian; Yan, Zhao; Wang, Jiabo; Xu, Zhihui; Sun, Zhang‐Hua; Zheng, Quanfu; Li, Ruisheng; Zhang, Ping; Li, Jianyu; Song, Xin; Xiao, Xiaohe

doi:10.1016/j.tca.2013.07.031

Cited by 9 publications

(8 citation statements)

References 18 publications

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“…E. coli was inoculated in Luria-Bertani (LB) culture medium prepared by dissolving 10 g peptone, 5 g yeast extract, and 5 g NaCl in 1,000 ml ultra-pure water (pH 7.0–7.2) and sterilized by autoclaving at 0.1 MPa and 121°C for 30 min and then stored in a refrigerator at 4°C (Zhao et al, 2011 ). Before microcalorimetric measurements, E. coli was added to a 50 ml sterilized flask containing 30 ml LB culture medium and then incubated in incubator shaker for 2.5 h at 37°C (Liu T. et al, 2013 ) with the rotation speed at 75 rpm.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have showed that the ethanol extracts of the leaves of D. dao demonstrate anti- Staphylococcus aureus and anti- Bacillus subtitles activities (Khan and Omoloso, 2002 ). The subsequent studies from our research team have also indicated that the different extracts from the leaves of D. dao by means of systematic solvent extraction (including petroleum ether, chloroform, ethyl acetate, n-butanol, and water) show anti- Escherichia coli (the greatest IC 50 value is 83.93 μg·ml −1) and anti- Staphylococcus aureus (the greatest IC 0 value is 98.5 μg·ml −1 ) activities, especially the ethyl acetate fraction, the main components of which were flavonoids, including Cianidanol, L-Epicatechin, Quercetin, and Luteolin (Liu T. et al, 2013 ; Zhao et al, 2015 ), exhibit significant anti- Pseudomonas aeruginosa (IC 50 = 18.06 μg·ml −1 ) activity (Wu et al, 2015 ). The metabolic power-time ( P-t ) curves were established (Kong et al, 2009b ; Braissant et al, 2010 ; Ren et al, 2010 ; Kabanova et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

“…The basic principle of microcalorimetry is that the growth or/and metabolism of organism consistent with the change of heat is real-time online recorded and further evaluated. Under a set of growth conditions, unique power—time curves presented by the heat produced in the growth of cells could be analyzed quantitatively and qualitatively by the microcalorimeter (Zhao et al, 2010 , 2011 ; Liu T. et al, 2013 ; Zheng et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Antibacterial Effects of Flavonoid Combination from the Leaves of Dracontomelon dao by Microcalorimetry and the Quadratic Rotary Combination Design

Xia

et al. 2017

Front. Pharmacol.

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Skin infectious disease is a common public health problem due to the emergence of drug-resistant bacteria caused by the antibiotic misuse. Dracontomelon dao (Blanco) Merr. et Rolfe, a traditional Chinese medicine, has been used for the treatment of various skin infectious diseases over 1000 of years. Previous reports have demonstrated that the leaves of D. dao present favorable antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtitles. The flavonoids are the main components of the ethyl acetate extract of D. dao leaf. However, the correlation between flavonoids and antibacterial activities is yet to be determined. In this study, the combined antibacterial activities of these flavonoids were investigated. Three samples with the different concentrations of flavonoids (S1–S3) were obtained. By microcalorimetric measurements, the results showed that the IC50 value of S2 was lower than those of S1 and S3. The contents of main flavonoids (including Luteolin, L-Epicatechin, Cianidanol, and Quercetin) in S1–S3 were various, confirmed by the method of the Ultra High Performance Liquid Chromatography (UPLC). Based on the method of quadratic general rotary unitized design, the antibacterial effect of single flavonoid, and the potential synergistic effects between Luteolin and Quercetin, Luteolin and Cianidanol were calculated, which were also proved by microcalorimetric analysis. The antibacterial activities of main flavonoids were Luteolin > Cianidanol > Quercetin > L-Epicatechin. Meanwhile, the synergistic effects of Luteolin and Cianidanol (PL+C = 1.425), Quercetin and Luteolin (PL+Q = 1.129) on anti-microbial activity were validated. Finally, we found that the contents of Luteolin, L-Epicatechin, Cianidanol, Quercetin were 1061.00–1061.00, 189.14–262.86, 15,990.33–16,973.62, 6799.67–7662.64 ng·ml−1 respectively, with the antibacterial rate over 60.00%. In conclusion, this study could provide reference for quality evaluation and pharmacodynamics research of D. dao.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Antibacterial Effects of Flavonoid Combination from the Leaves of Dracontomelon dao by Microcalorimetry and the Quadratic Rotary Combination Design

Xia

et al. 2017

Front. Pharmacol.

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“…002%-0. 055 0%)) [36]. At room temperature, aconite powder (1.5g) mixed with yellow rice wine (1.5g) shaped using a mold (1 cm in diameter and 0.45 cm thick) (ratio: 1:1) and fixed it for 10 minutes.…”

Section: Treatmentmentioning

confidence: 99%

Herb-partitioned moxibustion alleviates colonic inflammation in Crohn’s disease rats by inhibiting hyperactivation of the NLRP3 inflammasome via regulation of the P2X7R-Pannexin-1 signaling pathway

et al. 2021

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Crohn’s disease is a chronic inflammatory bowel disease and the NLRP3 inflammasome plays an important role in Crohn’s disease. Previous studies have shown that Herb-partitioned moxibustion treating (at Qihai (CV 6) and Tianshu (ST 25)) prevented the excessive activation of the NLRP3 inflammasome and repaired damaged colonic mucosa in Crohn’s disease. However, the mechanism by which Herb-partitioned moxibustion (at CV 6 and ST 25) regulates NLRP3 remains unclear. In this study, we treated Crohn’s disease rats with herb-partitioned moxibustion (at CV 6 and ST 25) to investigate the mechanism by which Herb-partitioned moxibustion regulates the colonic NLRP3 inflammasome by observing colon length, the colon macroscopic damage indexes, and the expression of ATP, P2X7R, Pannexin-1, NF-κBp65, NLRP3, ASC, caspase-1, IL-1β and IL-18 in the colon in Crohn’s disease. Here, this study shows that herb-partitioned moxibustion (at CV 6 and ST 25) can reduce colon macroscopic damage indexes and colon histopathological scores, alleviate colon shortening and block the abnormal activation of the NLRP3 inflammasome by inhibiting the ATP content and the expression of P2X7R, Pannexin-1 and NF-κBp65, thereby reducing the release of the downstream inflammatory cytokine IL-1β and ultimately suppressing colonic inflammation in Crohn’s disease rats. This study for the first time identifies the mechanism by which herb-partitioned moxibustion (at CV 6 and ST 25) may inhibit the abnormal activation of the NLRP3 inflammasome by inhibiting the P2X7R-Pannexin-1 signaling pathway in Crohn’s disease rats.

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“…Also, the ethanolic extract from the sapwood of D. dao exhibited antimicrobial activity against gramnegative bacteria: Salmonella typhimurium and Klebsiella pneumoniae; grampositive bacteria: S. aureus and Bacillus subtilis; and fungi: Candida albicans and Aspergillus niger (Dela Peña et al 2019). Several systematic solvent extractions of D. dao like petroleum ether, chloroform, ethyl acetate, n-butanol, and water exhibited significant anti-E. coli and anti-S. aureus (Liu et al 2013;Zhao et al 2015), and anti-Pseudomonas aeruginosa activities (Wu et al 2015). Moreover, essential oil of D. dao has been reported to have antitumor activity (Su et al 2008).…”

Section: Phytochemistrymentioning

confidence: 99%

Dracontomelon dao (Blanco) Merr. & Rolfe Anacardiaceae

Dapar¹

2020

Ethnobotany of Mountain Regions

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Action of crude Radix Aconiti Lateralis (Fuzi) and its processed products on splenic lymphocytes growth investigated by microcalorimetry

Cited by 9 publications

References 18 publications

Evaluation of the Antibacterial Effects of Flavonoid Combination from the Leaves of Dracontomelon dao by Microcalorimetry and the Quadratic Rotary Combination Design

Evaluation of the Antibacterial Effects of Flavonoid Combination from the Leaves of Dracontomelon dao by Microcalorimetry and the Quadratic Rotary Combination Design

Herb-partitioned moxibustion alleviates colonic inflammation in Crohn’s disease rats by inhibiting hyperactivation of the NLRP3 inflammasome via regulation of the P2X7R-Pannexin-1 signaling pathway

Dracontomelon dao (Blanco) Merr. & Rolfe Anacardiaceae

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