Action of aprotinin on the survival of adult cerebellar neurons in organ culture

Davis, Heather L.; Gascho, C.; Kiernan, J. A.

doi:10.1007/bf00696799

Cited by 8 publications

(3 citation statements)

References 7 publications

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“…Moreover, the ability of Fb to sequester bFGF and BDNF through its heparin‐binding domain (Martino et al ., ) possibly contributed to their retention within fibrin and to prolong their bioavailability to cells. The addition of aprotinin to cell culture media, a serine protease inhibitor herein used to delay fibrin degradation, may have also contributed to cell viability, as aprotinin was shown to exert neuroprotective effects in vitro (Davis et al ., ) and to increase cell viability of dissociated embryoid bodies (EBs)‐containing neural progenitors in Fb (Willerth et al ., ). ES‐NSPCs entrapped within Fb as single cells rapidly divided forming spherical‐shaped clone‐like multicellular clusters.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional culture of single embryonic stem-derived neural/stem progenitor cells in fibrin hydrogels: neuronal network formation and matrix remodelling

Bento

Quelhas

Oliveira

et al. 2016

J Tissue Eng Regen Med

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In an attempt to improve the efficacy of neural stem/progenitor cell (NSPC) based therapies, fibrin hydrogels are being explored to provide a favourable microenvironment for cell survival and differentiation following transplantation. In the present work, the ability of fibrin to support the survival, proliferation, and neuronal differentiation of NSPCs derived from embryonic stem (ES) cells under monolayer culture was explored. Single mouse ES-NSPCs were cultured within fibrin (fibrinogen concentration: 6 mg/ml) under neuronal differentiation conditions up to 14 days. The ES-NSPCs retained high cell viability and proliferated within small-sized spheroids. Neuronal differentiation was confirmed by an increase in the levels of βIII-tubulin and NF200 over time. At day 14, cell-matrix constructs mainly comprised NSPCs and neurons (46.5% βIII-tubulin cells). Gamma-aminobutyric acid (GABA)ergic and dopaminergic/noradrenergic neurons were also observed, along with a network of synaptic proteins. The ES-NSPCs expressed matriptase and secreted MMP-2/9, with MMP-2 activity increasing along time. Fibronectin, laminin and collagen type IV deposition was also detected. Fibrin gels prepared with higher fibrinogen concentrations (8/10 mg/ml) were less permissive to neurite extension and neuronal differentiation, possibly owing to their smaller pore area and higher rigidity. Overall, it is shown that ES-NSPCs within fibrin are able to establish neuronal networks and to remodel fibrin through MMP secretion and extracellular matrix (ECM) deposition. This three-dimensional (3D) culture system was also shown to support cell viability, neuronal differentiation and ECM deposition of human ES-NSPCs. The settled 3D platform is expected to constitute a valuable tool to develop fibrin-based hydrogels for ES-NSPC delivery into the injured central nervous system. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Three-dimensional culture of single embryonic stem-derived neural/stem progenitor cells in fibrin hydrogels: neuronal network formation and matrix remodelling

Bento

Quelhas

Oliveira

et al. 2016

J Tissue Eng Regen Med

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“…Aprotinin exerts various effects on tissue when used in a tissue culture model at extremely high concentrations (up to 10 7 KIU/L). 22,23 The effect of aprotinin on organ preservation-liver, kidney, and lung-has also been studied. [24][25][26][27][28][29] This proteinase inhibitor was shown to protect dog myocardium against ischemic injury in terms of diminished area of infarction after coronary artery ligation.…”

Section: Discussionmentioning

confidence: 99%

Aprotinin improves myocardial recovery after ischemia and reperfusion:

Gurevitch¹,

Barak²,

Hochhauser³

et al. 1994

The Journal of Thoracic and Cardiovascular Surgery

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Aprotinin improves myocardial recovery after ischemia and reperfusion Effects of the drug on isolated rat heartsThe effects of aprotinin, a protease inhibitor, on the ischemic and nonischemic isolated rat heart was investigated with the use of the modified Langendorlf model. During phase I of the study, hearts were perfused with either low-dose aprotinin (10 5 Kill/L), high-dose aprotinin (10 6 KIU /L), or normal saline solution added to modified Krebs-Henseleit solution. No statistically significant differences in contraction amplitude, contractility, coronary flow, and wet/dry heart weight ratio were observed among the three groups of hearts. In phase II, hearts were exposed to a 40-minute period of global ischemia at 31 0 C. Ischemic arrest was induced by warm cardioplegia. Before ischemia and during cardioplegia, hearts were perfused with either aprotinin 10 6 KIU/L (n = 10) or normal saline solution (n = 10) for .30 minutes. On reperfusion, recovery of hearts treated with aprotinin was significantly better than that of control hearts, as reflected by better contractility (analysis of variance, p = 0.011), higher coronary flow (p < 0.025), and lower creatine kinase levels (p <0.05). No statistically significant differences in contraction amplitude were observed between the two groups. When the effect of ischemia within each group of hearts was analyzed, the preserving effect of aprotinin was even more pronounced. In the control group, ischemia caused a decrease in contractility (p < 0.025) and a decrease in oxygen consumption (p = 0.006); by contrast, in the aprotinin group the preischemic values were maintained. Accordingly, we conclude that aprotinin at concentrations up to 10 6 KIU /L has no deleterious effect on normally perfused hearts and has a significant protective effect on the ischemic heart when used in high doses in the preischemic period. (J THoRAe CARDIOVASC SURG 1994;108:109-18)

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“…and the foetal C.N.S. (Dunn, Davis, Gascho & Kiernan, 1975). Central white matter is more amenable to transplantation.…”

Section: (B) Transplantation Of Perzpheral ~O U Fmentioning

confidence: 99%

Hypotheses Concerned With Axonal Regeneration in the Mammalian Nervous System

Kiernan

1979

Biological Reviews

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257

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Action of aprotinin on the survival of adult cerebellar neurons in organ culture

Cited by 8 publications

References 7 publications

Three-dimensional culture of single embryonic stem-derived neural/stem progenitor cells in fibrin hydrogels: neuronal network formation and matrix remodelling

Three-dimensional culture of single embryonic stem-derived neural/stem progenitor cells in fibrin hydrogels: neuronal network formation and matrix remodelling

Aprotinin improves myocardial recovery after ischemia and reperfusion:

Hypotheses Concerned With Axonal Regeneration in the Mammalian Nervous System

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