Action of amphotericin B on the toad bladder: evidence for sodium transport along two pathways

Bentley, P. J.

doi:10.1113/jphysiol.1968.sp008531

Cited by 35 publications

(18 citation statements)

References 17 publications

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“…(2) By exposing the mucosal surface to Amphotericin B, a polyene antibiotic known to increase selectively the apical cellular permeability to Na+ ions (Lichenstein & Leaf, 1965;Bentley, 1968;Cremaschi, Henin, Meyer & Bacciola, 1977), it was possible to induce across the tissue large changes in p.d. and resistance which may interfere in turn with other exchanges, namely with Cl-.…”

Section: Introductionmentioning

confidence: 99%

Ion flux changes induced by voltage clamping or by amphotericin B in the isolated urinary bladder of the trout.

Fossat¹,

Lahlou²

1982

The Journal of Physiology

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SUMMARY1. When bathed with Ringer solutions on both sides in Ussing chambers, isolated urinary bladders of trout carry out an electrically silent transport of Na+ and Clions from mucosa to serosa. In the present experiments, an electrical gradient was set up across the epithelium mounted under these conditions either by using a voltage-clamp technique or by submitting the mucosal side to the polyene antibiotic Amphotericin B 2. With stable potential differences between -40 and + 40 mV applied to the tissue, both unidirectional fluxes (Jms and Jsm) for Na+ and for Cl-varied as a function of the electrical gradient. This indicates that a sizeable fraction of these ionic exchanges is due to passive diffusion across a low-resistance pathway.3. Amphotericin B (40 ,sg/ml.) applied to the mucosal side induced an immediate and large (up to 50 mV, serosa positive) potential difference and a sustained diminution in tissue resistance. These effects were strictly Na+-dependent and were reduced in the presence of ouabain. Therefore they are due to increased passive entry of Na+ along its electrochemical gradient across the cellular apical membrane. 4. The effect of Amphotericin B was enhanced when Cl-was replaced by an impermeant anion such as gluconate. Therefore the shunting effect of Cl-on the induced potential observed in the control conditions appears to be due to an increase in Cl-permeability across an anion pathway. Flux data indicate that this pathway is transcellular.

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Section: Introductionmentioning

confidence: 99%

Ion flux changes induced by voltage clamping or by amphotericin B in the isolated urinary bladder of the trout.

Fossat¹,

Lahlou²

1982

The Journal of Physiology

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“…An alternative mechanism could involve low permeability of the outer apical barrier of the epithelial cells. The polyene antibiotic amphotericin B can interact with sterols in the walls of such cells (Lichtenstein & Leaf, 1965;Bentley, 1968a) and so increases their permeability. When amphotericin B was placed in the solution bathing the outer surface of the skin a transmural potential difference appeared and a short-circuit current could be measured ( Fig.…”

Section: Resultsmentioning

confidence: 99%

The permeability of the skin of a neotenous urodele amphibian, the mudpuppy Necturus maculosus.

Bentley¹,

Yorio²

1977

The Journal of Physiology

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SUMMARY1. The permeability of the isolated skin of a neotenous urodele amphibian, the mudpuppy Necturus maculosus, to Na, C1, urea and water was measured.2. Unidirectional transcutaneous flux measurements and the action of ouabain and amiloride, showed that there was normally no active Cl or Na transport, nor a Cl/Cl exchange diffusion process.3. Amphotericin B initiated a transcutaneous potential difference and short-circuit current, which could be inhibited by ouabain.4. The short-circuit current was nearly equivalent to the net Na transport and this was also inhibited by ouabain.5. A transcutaneous active Na transport mechanism thus appears to be incipient in the mudpuppy but is limited by a low permeability of the outer barrier of the cells.6. Vasotocin increased the skin's diffusion permeability for water but had no effect on the influx of Na or urea. 7. The function of Necturus skin is in several respects unique compared to that of other amphibians.

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“…AmB increased Na transport (4, 23, 26) but diminishes the ADH effect on Na transport in the toad bladder (4,28), it is not clear whether this AmB induced impairment of ADH action reflects the reduction of water transfer per se or the depression of the Na transport response to ADH. However, qual itatively similar results were obtained when Na was replaced by choline in both mucosal and serosal media or only in the mucosal bathing medium, although the magnitudes Time, min Fig.…”

Section: Effects Of Amb On Toad Urinary Bladder Water Permeabilitymentioning

confidence: 95%

Interaction of Amphotericin B and Vasopressin in the Rat Kidney and the Toad Urinary Bladder

Park¹,

Hong²,

Solomon³

1978

Kidney Blood Press Res

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The interaction of amphotericin B (AmB) and vasopressin (ADH) was studied in the rat and the toad urinary bladder. In the anesthetized rat infused with a sustaining dose of ADH, AmB administration resulted in a decrease in urine osmolality and increases in urine flow and fractional water and Na excretions. However, the urine flow for a given osmolar clearance was higher after AmB than before, indicating that free water reabsorption is inhibited by AmB. Micropuncture experiments suggested an inhibition of proximal Na reabsorption after AmB. In the toad bladder, AmB added to the mucosal side enhanced osmotic water flux and impaired hydroosmotic action of ADH. These results indicate a functional antagonism between AmB and ADH in the renal tisssue.

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Action of amphotericin B on the toad bladder: evidence for sodium transport along two pathways

Cited by 35 publications

References 17 publications

Ion flux changes induced by voltage clamping or by amphotericin B in the isolated urinary bladder of the trout.

Ion flux changes induced by voltage clamping or by amphotericin B in the isolated urinary bladder of the trout.

The permeability of the skin of a neotenous urodele amphibian, the mudpuppy Necturus maculosus.

Interaction of Amphotericin B and Vasopressin in the Rat Kidney and the Toad Urinary Bladder

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