Action of Aldosterone on Citrate Synthase in Cultured Renal Collecting Duct Cells

Minuth, Will W.; Struck, Michael; Zwanzig, Marianne; Groß, Peter

doi:10.1159/000173182

Cited by 3 publications

(5 citation statements)

References 18 publications

(44 reference statements)

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“…Others have also found minimal or no increase in citrate synthase activity in cultured cells that respond to aldosterone by increasing Na' transport. Enzyme activity is not stimulated in either aldosterone-treated A6 cells or cultured toad bladder cells (33,34), and only a 30% rise has been demonstrated in primary cultures of rabbit CCD (35). These results in cultured cells stand in contrast to the marked stimulation seen in native tubules (32).…”

Section: Discussionmentioning

confidence: 74%

Cellular responses to steroids in the enhancement of Na+ transport by rat collecting duct cells in culture. Differences between glucocorticoid and mineralocorticoid hormones.

Laplace

Husted²,

Stokes³

1992

J. Clin. Invest.

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It has recently been discovered that both mineralocorticoid (MC) and glucocorticoid (GC) hormones can stimulate electrogenic Na' absorption by mammalian collecting duct cells in culture. In primary cultures of rat inner medullary collecting duct (IMCD) cells, 24-h incubation with either MC or GC agonist stimulates Na+ transport approximately threefold. We have now determined that the effects were not additive, but the time courses were different. As aldosterone is known to stimulate citrate synthase, Na+/K' ATPase activity, and ouabain binding in cortical collecting duct principal cells, we determined the effects of steroids on these parameters in IMCD cells. MC and GC agonists both produced a small increase in citrate synthase activity. There was no increase in Na + / K+ ATPase activity but specific ouabain binding was increased more than twofold by either agonist. To determine the role ofapical Na+ entry in the steroid-induced effects, the Na+ channel inhibitor, benzamil, was used. Benzamil did not alter the stimulation of citrate synthase activity by either steroid. In contrast, GC stimulation of ouabain binding was prevented by benzamil, whereas MC stimulation was not. We conclude that there are differences in the way that MC and GC hormones produce an increased Na+ transport. Both appear to produce translocation (or activation) of pumps into the basolateral membrane. GC stimulation of pump translocation requires increased Na+ entry whereas MC stimulation does not. (J. Clin. Invest. 1992.

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Section: Discussionmentioning

confidence: 74%

Cellular responses to steroids in the enhancement of Na+ transport by rat collecting duct cells in culture. Differences between glucocorticoid and mineralocorticoid hormones.

Laplace

Husted²,

Stokes³

1992

J. Clin. Invest.

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“…Cortical explants from the kidney of newborn New Zealand rabbits were isolated as described elsewhere [ 10, [23][24][25][26][27][28]. The explants consisted of a piece of capsula fibrosa with adherent nephrogenic blastema containing S-shaped bodies and collecting duct anlagen.…”

Section: Cellsmentioning

confidence: 99%

“…To overcome this problem, we developed a technique for culture of a clearly defined, highly differentiated, and electrically " tight" collecting duct epithelium. It consisted of "principal cells" only [lo, [23][24][25][26][27][28]. We used this system of cultured collecting duct cells from mammalian kidney to investigate the appearance of two collecting-duct-specific proteins (PcD 2 and 3) [7] in response to chronic aldosterone and AVP administration and in close correlation with electrophysiological properties.…”

Section: The Problem Of a Suitable Model For Investigation Of Mammalimentioning

confidence: 99%

“…6a, c), the mature principal cell of the renal collecting duct [14, 201 ( Fig. 6b, d), nor the cultured epithelial cell [24] can be distinguished by morphological characteristics or other cell-biological markers [23, 24, 281. Antinatriuretic hormones modulated electrical properties and insertion of plasma membrane proteins Chronic application of antinatriuretic hormones demonstrated that both AVP and aldosterone had a direct influ-ence on measurable electrical properties of the cultured epithelium (Table 1). While control epithelia demonstrated chracteristics of electrical leakiness, a 10-day treatment with AVP, aldosterone, or aldosterone plus AVP was associated with electrical characteristics of a " tight"-epithelium (Rte > 0.5 k Q cm').…”

Section: Specifity Of the Mabs CD 2 And Cd 3 For The Differentiated Cmentioning

confidence: 99%

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Appearance of specific proteins in the apical plasma membrane of cultured renal collecting duct principal cell epithelium after chronic administration of aldosterone and arginine vasopressin

1988

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“…[1][2][3][4][5] Adrenalectomy reduces renal citrate synthase activity, and the administration of aldosterone to adrenalectomized animals restores citrate synthase activity. 4 These findings arose from an ongoing effort to identify aldosterone-induced proteins (ie, proteins synthesized in response to the transcription factor activity of the occupied mineralocorticoid receptor) and to determine how these proteins mediate the classic action of mineralocorticoids: renal sodium reabsorption.…”

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confidence: 99%

Role of Citrate Synthase in Aldosterone-Mediated Sodium Reabsorption

et al. 2000

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Abstract-Aldosterone and other mineralocorticoids increase citrate synthase activity in the kidney and enhance renal sodium reabsorption, but it is unclear whether the increased citrate synthase activity is involved in renal sodium transport. We used the Wistar-Furth rat, an inbred strain found to be deficient in renal citrate synthase activity, as an experimental model to investigate this issue. We confirmed that renal citrate synthase activity from adrenalectomized Wistar-Furth rats was decreased compared with that from control Wistar rats (by 28% T he mineralocorticoid aldosterone stimulates activity of certain key enzymes in the tricarboxylic acid cycle (eg, citrate synthase) in the rat kidney. 1-5 Adrenalectomy reduces renal citrate synthase activity, and the administration of aldosterone to adrenalectomized animals restores citrate synthase activity. 4 These findings arose from an ongoing effort to identify aldosterone-induced proteins (ie, proteins synthesized in response to the transcription factor activity of the occupied mineralocorticoid receptor) and to determine how these proteins mediate the classic action of mineralocorticoids: renal sodium reabsorption. It has been hypothesized that enhancement of citrate synthase activity increases ATP formation and, subsequently, activity of Na ϩ ,K ϩ -ATPase and sodium reabsorption in the distal nephron. This schema is consistent with the "energy hypothesis" of aldosterone action.However, the correlation of aldosterone-induced enhancement of renal citrate synthase activity with aldosteroneinduced stimulation of sodium reabsorption has been investigated in only a few studies, and the results have been inconsistent. At 3 to 4 hours after aldosterone was injected into rats, both a maximum increase in renal citrate synthase activity and a maximum decrease in urinary sodium-topotassium ratio were observed. 3 Other studies have been performed in cultured cell lines. In toad bladder and toad kidney cells, aldosterone stimulated sodium transport without an increase in citrate synthase activity. 6 In another study, aldosterone stimulated both vectorial sodium transport and citrate synthase activity in toad bladder cells, whereas aldosterone stimulated sodium transport in the absence of an increase in citrate synthase activity in A6 cells. 7 In the present study, we used Wistar-Furth rats (WF) as an experimental model to investigate the role of citrate synthase activity in aldosterone-mediated sodium reabsorption. WF, which were bred from normal Wistar rats (W), have been shown to be resistant to the development of mineralocorticoid-excess hypertension. 8 -13 During the biochemical evaluation of the mechanisms that underlie this resistance to mineralocorticoids, we observed that basal and aldosterone-stimulated citrate synthase activities in whole kidney were markedly reduced in WF compared with W. 13

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Action of Aldosterone on Citrate Synthase in Cultured Renal Collecting Duct Cells

Cited by 3 publications

References 18 publications

Cellular responses to steroids in the enhancement of Na+ transport by rat collecting duct cells in culture. Differences between glucocorticoid and mineralocorticoid hormones.

Cellular responses to steroids in the enhancement of Na+ transport by rat collecting duct cells in culture. Differences between glucocorticoid and mineralocorticoid hormones.

Appearance of specific proteins in the apical plasma membrane of cultured renal collecting duct principal cell epithelium after chronic administration of aldosterone and arginine vasopressin

Role of Citrate Synthase in Aldosterone-Mediated Sodium Reabsorption

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