Actinopyrone D, a new downregulator of the molecular chaperone GRP78 from Streptomyces sp.

Hayakawa, Yoichi; Saito, Junki; Izawa, Masako; Shin‐ya, Kazuo

doi:10.1038/ja.2014.76

Cited by 11 publications

(9 citation statements)

References 16 publications

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“…B. abortus takes advantage of VceC to interact with the ER chaperone GRP78 and localizes to the ER in HeLa cells (de Jong et al, 2013 ). Streptomyces inhibits GRP78 protein expression and induces cell death under ER stress (Hayakawa et al, 2014 ). Currently, Nexavar/Stivarga/Votrient is used to target GRP78 in the treatment of human malignancies, viral infections and bacterial diseases (Roberts et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Brucella suis Vaccine Strain 2 Induces Endoplasmic Reticulum Stress that Affects Intracellular Replication in Goat Trophoblast Cells In vitro

Wang

Lin

et al. 2016

Front. Cell. Infect. Microbiol.

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Brucella has been reported to impair placental trophoblasts, a cellular target where Brucella efficiently replicates in association with the endoplasmic reticulum (ER), and ultimately trigger abortion in pregnant animals. However, the precise effects of Brucella on trophoblast cells remain unclear. Here, we describe the infection and replication of Brucella suis vaccine strain 2 (B.suis.S2) in goat trophoblast cells (GTCs) and the cellular and molecular responses induced in vitro. Our studies demonstrated that B.suis.S2 was able to infect and proliferate to high titers, hamper the proliferation of GTCs and induce apoptosis due to ER stress. Tunicamycin (Tm), a pharmacological chaperone that strongly mounts ER stress-induced apoptosis, inhibited B.suis.S2 replication in GTCs. In addition, 4 phenyl butyric acid (4-PBA), a pharmacological chaperone that alleviates ER stress-induced apoptosis, significantly enhanced B.suis.S2 replication in GTCs. The Unfolded Protein Response (UPR) chaperone molecule GRP78 also promoted B.suis.S2 proliferation in GTCs by inhibiting ER stress-induced apoptosis. We also discovered that the IRE1 pathway, but not the PERK or ATF6 pathway, was activated in the process. However, decreasing the expression of phosphoIRE1α and IRE1α proteins with Irestatin 9389 (IRE1 antagonist) in GTCs did not affect the proliferation of B.suis.S2. Although GTC implantation was not affected upon B.suis.S2 infection, progesterone secretion was suppressed, and prolactin and estrogen secretion increased; these effects were accompanied by changes in the expression of genes encoding key steroidogenic enzymes. This study systematically explored the mechanisms of abortion in Brucella infection from the viewpoint of pathogen invasion, ER stress and reproductive endocrinology. Our findings may provide new insight for understanding the mechanisms involved in goat abortions caused by Brucella infection.

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Section: Discussionmentioning

confidence: 99%

Brucella suis Vaccine Strain 2 Induces Endoplasmic Reticulum Stress that Affects Intracellular Replication in Goat Trophoblast Cells In vitro

Wang

Lin

et al. 2016

Front. Cell. Infect. Microbiol.

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“…14 The only difference of pyrone ring formation in the biosynthetic pathway is the absence of amidation. 15 Actinopyrones with α -methoxy- γ -pyrone ring, such as actinopyrones A-D, 16,17 M050511,PM050463, PM060054, and PM060431, 18 were also discovered from Streptomyces strains. Not only the chemical structural similarity, actinopyrones showed resembling antibiotic/antitumor activities and related biosynthetic pathway with piericidins.…”

Section: Resultsmentioning

confidence: 99%

Two new α-Methoxy-γ-Pyrones From the Mangrove Sediment-Derived Streptomyces psammoticus SCSIO NS126

Zhou

et al. 2021

Natural Product Communications

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Two new α-methoxy- γ-pyrone analogs, 2-methoxy-3-methyl-5,6-diethyl- γ-pyrone (2) and 2-methoxy-3,5-dimethyl-6-propyl- γ-pyrone (3), together with 2-methoxy-3,5-dimethyl-6-ethyl- γ-pyrone (1), firstly isolated from natural sources, were obtained from the EtOAc-solube extract of the mangrove sediment-derived actinomycete strain Streptomyces psammoticus SCSIO NS126, under the optimized fermentation conditions. Their structures were elucidated by detailed spectroscopic analysis and by comparison of their spectroscopic data with those reported in the literature. Those α-methoxy-γ-pyrones were evaluated for their acetylcholinesterase inhibitory activity; however, none of them exhibited obvious activity. Moreover, their biosynthetic relationship with piericidins was also discussed.

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“…A further ECD analysis led to the establishment of the absolute configuration of 95. effect against glutamate-induced cytotoxicity in HT22 murine hippocampal neuronal cells. Besides, two 4-pyrone propionates, 99 and 100, constructed a relatively long side chain, were produced by an actinomycete Streptomyces sp [58,59]. 99 dose-dependently inhibited luciferase expression in 2deoxyglucose treated HT1080 human fibrosarcoma cells (IC50 = 7.8 nM) and it could also inhibit GRP78 protein expression and induce cell death under endoplasmic reticulum stress.…”

Section: Mar Drugs 2020 18 X 6 Of 23mentioning

confidence: 99%

Natural Polypropionates in 1999–2020: An Overview of Chemical and Biological Diversity

Liu

Zhang

2020

Marine Drugs

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Natural polypropionates (PPs) are a large subgroup of polyketides with diverse structural features and bioactivities. Most of the PPs are discovered from marine organisms including mollusks, fungi and actinomycetes, while some of them are also isolated from terrestrial resources. An increasing number of studies about PPs have been carried out in the past two decades and an updated review is needed. In this current review, we summarize the chemical structures and biological activities of 164 natural PPs reported in 67 research papers from 1999 to 2020. The isolation, structural features and bioactivities of these PPs are discussed in detail. The chemical diversity, bioactive diversity, biodiversity and the relationship between chemical classes and the bioactivities are also concluded.

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Actinopyrone D, a new downregulator of the molecular chaperone GRP78 from Streptomyces sp.

Cited by 11 publications

References 16 publications

Brucella suis Vaccine Strain 2 Induces Endoplasmic Reticulum Stress that Affects Intracellular Replication in Goat Trophoblast Cells In vitro

Brucella suis Vaccine Strain 2 Induces Endoplasmic Reticulum Stress that Affects Intracellular Replication in Goat Trophoblast Cells In vitro

Two new α-Methoxy-γ-Pyrones From the Mangrove Sediment-Derived Streptomyces psammoticus SCSIO NS126

Natural Polypropionates in 1999–2020: An Overview of Chemical and Biological Diversity

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