Actinopolyspora iraqiensis sp. nov., a New Halophilic Actinomycete Isolated from Soil

Abstract

Halophilic actinomycete strains, IQ-HIT (T = type strain) and IQ-H2, were isolated from extremely saline soil samples in Iraq. Besides morphological properties, strains IQ-HIT and IQ-H2 have a type IV cell wall, type PI11 phospholipid, and menaquinone MK-9 (H4, H6), which are characteristic of Actinopolyspora spp. In addition, both strains required high concentrations of sodium chloride (10 to 15% [wt/vol] and 15 to 25% [wt/vol] NaCl, respectively), for optimal growth. However, strains IQ-HIT and IQ-H2 differed from other previously described species of the genus Actinopolyspora in cultural, morphological, and physiological characteristics. In addition, the size of the internal transcribed spacer in the rDNA (gene encoding rRNA) of IQ-HIT was different from those of known published species, while the size of the internal transcribed spacer of IQ-H2 was similar to that of ActinopoZyspora halophila JCM 327ST. Thus, we propose strain IQ-HIT as a new species and name it Actinopolyspora iraqiensis sp. nov. Strain IQ-HIT has been deposited in the culture collection

Actinopolyspora xinjiangensis sp. nov., a novel exteremely halophilic actinomycete isolated from a salt lake in Xinjiang, China

“…Data are from this study and from Gochnauer et al (1975), Yoshida et al (1991) and Ruan et al (1994). ?, positive; -, negative.…”

“…Susceptible to chloramphenicol (30 lg), nitrofurantoin (300 lg), ampicillin (10 lg), rifampicin (30 lg), carbenicillin (100 lg), tobramycin (10 lg), tetracycline (30 lg), novobiocin (30 lg), vancomycin (30 lg) and lincomycin (2 lg); but not Gochnauer et al (1975), Yoshida et al (1991) and Ruan et al (1994) Data are from this study and from Gochnauer et al (1975), Yoshida et al (1991) and Ruan et al (1994). The vegetative mycelium of strain TRM 40136 T was well developed, irregularly branched.…”

Novel Bioactive Metabolites from a Marine Derived Bacterium Nocardia sp. ALAA 2000

“…Chemotaxonomically: The presence of the isomeric form of diaminopimelic acid, and the composition of cell-wall sugars was analyzed by the previous method [21]. Polar lipids extracted, examined by two dimensional thin layer chromatography, the frist dimension with chloroform/ methanol/water (65 : 24 : 4) and in the second dimension with chloroform/methanol/acetic acid/water (80 : 12 : 15 : 4).…”

Taxonomy and antimicrobial activities of two novel halophilic Saccharomonospora strains isolated in Algerian Sahara soils

“…Another halophilic species, "Actinopolyspora iraqiensis" (Ruan et al 1994) was recently proposed to be reclassified as Saccharomonospora iraqiensis by S.K. Tang and co-workers (2007; unpublished data; details in http://www.ncbi.nlm.nih.gov/ nuccore/125975802.…”