Actinophytocola timorensis sp. nov. and Actinophytocola corallina sp. nov., isolated from soil

Abstract

Two actinomycete strains, ID05-A0653T and ID06-A0464T, were isolated from soils of West Timor and Lombok island, respectively, in Indonesia. 16S rRNA gene sequence analysis clearly demonstrated that the isolates belonged to the family Pseudonocardiaceae and were closely related to the genus Actinophytocola. Strains ID05-A0653T and ID06-A0464T exhibited 98.1 and 98.2 % 16S rRNA gene sequence similarity, respectively, with Actinophytocola oryzae GMKU 367T. The isolates grew well on ISP media and produced white aerial mycelium. Short spore chains were formed directly on the substrate mycelium. The isolates contained meso-diaminopimelic acid, arabinose and galactose as cell-wall components, MK-9(H4) as the sole isoprenoid quinone, iso-C16 : 0 as the major cellular fatty acid and phosphatidylethanolamine as the diagnostic polar lipid. The DNA G+C contents of strains ID05-A0653T and ID06-A0464T were 69.7 and 71.2 mol%, respectively. On the basis of phenotypic characteristics, DNA–DNA relatedness and 16S rRNA gene sequence comparisons, strains ID05-A0653T and ID06-A0464T each represent a novel species of the genus Actinophytocola, for which the names Actinophytocola timorensis sp. nov. (type strain ID05-A0653T = BTCC B-673T = NBRC 105524T) and Actinophytocola corallina sp. nov. (type strain ID06-A0464T = BTCC B-674T = NBRC 105525T) are proposed.

Actinophytocola sediminis sp. nov., an actinomycete isolated from a marine sediment

“…At maturity, spore chains with irregular round spores are borne on aerial hyphae. Growth occurs at pH 6.0-9.0 (optimum pH 8.0), 10-30 u C (optimum 25 u C) and with 0-6.0 % (w/v) NaCl (optimum (Ara et al, 2011;Guo et al, 2011); 4, A. corallina NBRC 105525 T (Guo et al, 2011;Otoguro et al, 2011); 5, A. oryzae NBRC 105245 T (Guo et al, 2011;Indananda et al, 2010); 6, A. timorensis NBRC 105524 T (Guo et al, 2011;Otoguro et al, 2011). All strains contain meso-diaminopimelic acid.…”

“…Currently, the genus Actinophytocola contains five species with validly published names, which were isolated from different habitats. Actinophytocola oryzae was isolated from the roots of Thai glutinous rice plants (Indananda et al, 2010), while Actinophytocola timorensis (Otoguro et al, 2011), Actinophytocola corallina (Otoguro et al, 2011), Actinophytocola burenkhanensis (Ara et al, 2011) and Actinophytocola xinjiangensis (Guo et al, 2011) were from soils. Members of the genus grow slowly, form nonfragmented substrate mycelium and, on some media, produce aerial mycelium that fragments into spore chains or spore chain-like structures.…”

“…The cell wall contains mesodiaminopimelic acid and mycolic acids are absent. The major fatty acid, diagnostic menaquinone and polar phospholipid are iso-C 16 : 0 , MK-9(H 4 ) and phosphatidylethanolamine, respectively (Indananda et al, 2010;Ara et al, 2011;Otoguro et al, 2011;Guo et al, 2011). However, A. xinjiangensis contains more MK-10(H 2 ) (76.5 %) than it does MK-9(H 4 ) (23.5 %), while the other species have only MK-9(H 4 ).…”

Actinophytocola algeriensis sp. nov., an actinobacterium isolated from Saharan soil

“…Its first species, Actinophytocola oryzae, was isolated from the roots of Thai glutinous rice plants. At the time of writing, this genus contains seven species with validly published names: Actinophytocola oryzae (Indananda et al, 2010) as the type species, Actinophytocola timorensis and Actinophytocola corallina (Otoguro et al, 2011), Actinophytocola burenkhanensis (Ara et al, 2011), Actinophytocola xinjiangensis (Xiaoxuan et al, 2011), Actinophytocola sediminis and Actinophytocola gilvus (Sun et al, 2014). Members of this genus are aerobic, Gramstain-positive and non-motile.…”

Actinophytocola gilvus sp. nov., isolated from desert soil crusts, and emended description of the genus Actinophytocola Indananda et al. 2010

“…Actinophytocola oryzae was first described and proposed as the type species of the genus Actinophytocola by Indananda et al (2010), with a single strain isolated from the root of Thai glutinous rice plant. Later, four more species of the genus Actinophytocola from different types of soil samples were identified (Otoguro et al, 2011;Ara et al, 2011;Guo et al, 2011). One of the genus-specific morphological features is that no sporangium-like structures are produced.…”