Abstract:The use of dyes in culture media for distinguishing brown and white woodrotting fungi. Ann. Botany, 12, 53-64. RALPH, B. J. F. 1949 The chemistry of wood-rotting fungi. Ph.D. thesis, University of Liverpool, England. RICHBERG, C. G. AND JENNISON, M. W. 1956 Vitamin content of the mycelium of wood-rotting Basidiomycetes.
“…Streptomyces antibioticus (ATCC 14888, Rutgers Institute for Microbiology Culture Collection no. 3720) was grown in soya peptone medium as described (7). Large quantities of mycelia were obtained by use of the facilities of the New England Enzyme Center.…”
A procedure for the preparation of relatively large quantities of highly purified phenoxazinone synthetase from
Streptomyces antibioticus
is described. Enzyme preparations consisted of multiple forms, as determined by polyacrylamide gel electrophoresis. Each of the electrophoretically separable forms catalyzed the oxidation of catechols, ferrocyanide, and ethylenic thiols, in addition to
o
-aminophenols.
“…Streptomyces antibioticus (ATCC 14888, Rutgers Institute for Microbiology Culture Collection no. 3720) was grown in soya peptone medium as described (7). Large quantities of mycelia were obtained by use of the facilities of the New England Enzyme Center.…”
A procedure for the preparation of relatively large quantities of highly purified phenoxazinone synthetase from
Streptomyces antibioticus
is described. Enzyme preparations consisted of multiple forms, as determined by polyacrylamide gel electrophoresis. Each of the electrophoretically separable forms catalyzed the oxidation of catechols, ferrocyanide, and ethylenic thiols, in addition to
o
-aminophenols.
“…described previously (Goss and Katz, 1957;Katz, Pienta, and Sivak, 1958;Katz and Goss, 1959). Actinomycins IV, VI, and VII possess similar antimicrobial activity; therefore, a crystalline preparation of actinomycin IV was employed as assay standard.…”
IKATZ, EDWARD (Rutgers, The State University, New Brunswick, N. J.), CLARENCE R. AWTALDRON, AND MARY Lou MEILONI. Role of valine and isoleucine as regulators of actinomycin peptide formation by Streptomyces chrysomallus.
“…We had observed quantitative variat.ions in the composition of an actinomycin mixture when different amino acids served as sole nitrogen sources * The investigation reported in this paper was supported in part by Research Grants E-2280 and E-3232 from the National Institute of Allergy and Infectious Diseases, Public Health Service, Bethesda, Md. 304 (Goss and Katz, 1957;Katz et ak., 1958). As the effects usually were limited and there appeared to be neither a chemical nor a biochemical relationship between the amino acid used and the change observed, we assumed that the nitrogen compound influenced chiefly the growth and metabolism of the organism and only indirectly antibiotic synthesis.…”
Section: Controlled Riosyntlzesis Of the Actinomycinsmentioning
confidence: 99%
“…( 5 ) Simultaneous synthesis of several closely related antibiotics by an organism, each differing only at one amino acid site. Usually one component of a mixture is synthesized in major amounts (Goss and Katz, 1957). What determines the extent to which each of the components is synthesized?…”
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