Actinomycetes in submerged culture

Whitaker, Allan

doi:10.1007/bf02922146

Cited by 33 publications

(15 citation statements)

References 77 publications

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“…The results at 10 6 spore/mL initial spore concentration are in line with a previous report by Heydarian et al (1997) for another strain of S. erythraea. An increase in the proportion of clump/dispersed growth morphology with an increase in initial spore concentration has also been reported for other filamentous actinomycetes (Whitaker, 1992;ElEnshasy et al, 2000). The rheological analysis indicated that, in the runs at 10 3 and 10 4 spore/mL initial spore concentrationin which pellet was the dominant morphology the cultivation broth exhibited Newtonian characteristics (Figure 3a).…”

Section: Rheological Measurementssupporting

confidence: 58%

“…The information available about the morphology of actinomycetes grown in submerged culture is restricted, even though this group of microorganisms is used to produce many of the antibiotics in current use (Whitaker, 1992). The morphology of these filamentous microorganisms is an important factor when they are employed in the industrial production of secondary metabolites, such as antibiotics.…”

Section: Introductionmentioning

confidence: 99%

“…For example, a mycelial form of Streptomyces griseus is required for streptomycin production, but pelleted and fragmented forms are undesirable (Schatz and Waksman, 1945). On the other hand, pellets of S. nigrificans have been shown to produce more glucose isomerase than the mycelial form (Whitaker, 1992).…”

Section: Introductionmentioning

confidence: 99%

“…One of the factors that has been reported to affect the morphology of filamentous microorganisms is the inoculum size (Whitaker, 1992; (Glazebrook and Vining, 1992;Lawton et al, 1989;Smith and Calam, 1980;Tucker and Thomas, 1992;Tucker and Thomas, 1994). An inoculum with high concentration of viable spores tends to produce a disperse form of growth, whereas the use of an inoculum with low spore concentration normally results in pellet formation (Whitaker, 1992).…”

Section: Introductionmentioning

confidence: 99%

“…An inoculum with high concentration of viable spores tends to produce a disperse form of growth, whereas the use of an inoculum with low spore concentration normally results in pellet formation (Whitaker, 1992). The inoculum spore concentration, in which the transition from predominantly pellet to a predominantly clump or dispersed mycelial form occurs, depends on the strain and can be affected by nutrient concentrations and other fermentation conditions (Braun and VechtLifshitz, 1991).…”

Section: Introductionmentioning

confidence: 99%

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The inter-relationship between inoculum concentration, morphology, rheology and erythromycin productivity in submerged cultivation of Saccharopolyspora erythraea

Ghojavand

Bonakdarpour

Heydarian

et al. 2011

Braz. J. Chem. Eng.

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-Submerged cultivation of Saccharopolyspora erythraea, at different initial spore concentrations, was carried out to study the inter-relationship between inoculum concentration, morphology, rheology and erythromycin production. Pellet morphology was dominant in runs at 10 3 and 10 4 spore/ml initial spore concentrations, whereas there was a significant presence of clump morphology in runs at initial spore concentrations of 10 5 -10 7 spore/ml. The S. erythraea cultivation broths exhibited Newtonian rheology in runs at initial spore concentrations of 10 3 and 10 4 spore/ml, whereas at higher initial spore concentrations the rheological data could be fitted with the power law model. Runs in which clump morphology was predominant resulted in the highest erythromycin productivities. The findings of the present work suggest that the predominance of clump morphology, smaller sized clumps and, in the case of non-Newtonian S. erythraea cultivation broths, a decrease in viscosity enhance erythromycin production.

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Section: Rheological Measurementssupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The inter-relationship between inoculum concentration, morphology, rheology and erythromycin productivity in submerged cultivation of Saccharopolyspora erythraea

Ghojavand

Bonakdarpour

Heydarian

et al. 2011

Braz. J. Chem. Eng.

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Vancomycin production in batch and continuous culture

McIntyre

Bull

Bunch

2000

Biotechnol. Bioeng.

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Macroparticle‐enhanced cultivation of Lentzea aerocolonigenes: Variation of mechanical stress and combination with lecithin supplementation for a significantly increased rebeccamycin production

Schrinner

Schrader

Niebusch

et al. 2021

Biotech & Bioengineering

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The actinomycete Lentzea aerocolonigenes produces the antitumor antibiotic rebeccamycin. In previous studies the rebeccamycin production was significantly increased by the addition of glass beads during cultivation in different diameters between 0.5 and 2 mm and the induced mechanical stress by the glass beads was proposed to be responsible for the increased production. Thus, this study was conducted to be a systematic investigation of different parameters for macroparticle addition, such as bead diameter, concentration, and density (glass and ceramic) as well as shaking frequency, for a better understanding of the particle‐induced stress on L. aerocolonigenes. The induced stress for optimal rebeccamycin production can be estimated by a combination of stress energy and stress frequency. In addition, the macroparticle‐enhanced cultivation of L. aerocolonigenes was combined with soy lecithin addition to further increase the rebeccamycin concentration. With 100 g L−1 glass beads in a diameter of 969 µm and 5 g L−1 soy lecithin a concentration of 388 mg L−1 rebeccamycin was reached after 10 days of cultivation, which corresponds to the highest rebeccamycin concentrations achieved in shake flask cultivations of L. aerocolonigenes stated in literature so far.

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Actinomycetes in submerged culture

Cited by 33 publications

References 77 publications

The inter-relationship between inoculum concentration, morphology, rheology and erythromycin productivity in submerged cultivation of Saccharopolyspora erythraea

The inter-relationship between inoculum concentration, morphology, rheology and erythromycin productivity in submerged cultivation of Saccharopolyspora erythraea

Vancomycin production in batch and continuous culture

Macroparticle‐enhanced cultivation of Lentzea aerocolonigenes: Variation of mechanical stress and combination with lecithin supplementation for a significantly increased rebeccamycin production

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