Actinomyces israelii in radicular cysts: a molecular study

Gomes, Nathalia Rodrigues; Diniz, Marina Gonçalves; Pereira, Thaís dos Santos Fontes; Estrela, Carlos; Farias, Luiz de Mácêdo; Andrade, Bruno Augusto Benevenuto de; Gomes, Carolina Cavaliéri; Gomez, Ricardo Santiago

doi:10.1016/j.oooo.2017.02.006

Cited by 8 publications

(6 citation statements)

References 17 publications

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“…2017, Gomes et al . 2017). However, Actinomyces species are often found in periapical lesions in the format of aggregates that look like ‘star‐bursts’ when examined histologically (Siqueira, 2003).…”

Section: Discussionmentioning

confidence: 99%

Bacteria and virulence factors in periapical lesions associated with teeth following primary and secondary root canal treatment

et al. 2020

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Aim This cross‐sectional study aimed to investigate the microbial profile and to quantify the levels of endotoxins (LPS) and lipoteichoic acid (LTA) present in periapical lesions associated with root filled teeth and those that had received root canal retreatment. It also aimed to investigate the association between microorganisms and their virulence factors with clinical and radiographic features. Methodology Patients with periapical lesions in teeth with post‐treatment endodontic disease following primary root canal treatment (n = 19) and unsuccessful root canal retreatment (n = 13) were treatment planned for endodontic microsurgery, where the periapical lesions were collected. Clinical and radiographic data were also collected. For microbiological analysis, nested polymerase chain reaction was used to detect 17 bacterial species. Levels of LPS and LTA were determined using limulus amebocyte lysate and enzyme‐linked immunosorbent assays, respectively. The Student t‐test or Wilcoxon–Mann–Whitney tests were applied to compare the data on LPS and LTA with clinical and radiographic features. The associations between the clinical and radiographic features and the bacterial species were analysed using the Fisher’s exact test. A significance level of 5% was adopted. Results Bacterial DNA, LPS and LTA were detected in all samples. Parvimonas micra was the most commonly detected species in all groups, followed by Enterococcus faecalis, Fusobacterium nucleatum and Porphyromonas endodontalis. The type of endodontic treatment, whether a primary root canal treatment or retreatment, was not associated with the presence of any bacterial species in periapical lesions. The levels of LPS and LTA in periapical lesions of root filled teeth were not significantly different from those that had been retreated. Associations between the levels of LPS and LTA with clinical signs and symptoms were found. No association was found between specific bacteria and clinical features. Conclusion Periapical lesions associated with teeth after primary root canal treatment and retreatment had similar polymicrobial composition. The levels of LPS and LTA in periapical lesions associated with teeth after primary root canal treatment and retreatment were similar, and both were associated with the same symptomatology.

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Section: Discussionmentioning

confidence: 99%

Bacteria and virulence factors in periapical lesions associated with teeth following primary and secondary root canal treatment

et al. 2020

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“…A. israelii finding on culture was an unexpected result in this case, although something which should be considered where pleural aspiration demonstrates frank pus 9. The diagnosis of A. israelii can be a challenge to clinicians due to its presentation often being with non-specific symptoms but can be confirmed on microscopy and culture 10. Microscopically, the presence of gram-positive aggregates with surrounding neutrophils can be identified.…”

Section: Discussionmentioning

confidence: 72%

“…The unusual finding of A. israelii prompted some consideration of risk factors for developing this infection. There was no evidence that this patient was immunocompromised; however, he did have poor dentition requiring multiple dental procedures and is a current smoker with these being possible risks for development of A. israelii infection 10 11. It is not felt that his temporary housing situation contributed to the acquisition of this infection.…”

Section: Discussionmentioning

confidence: 93%

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Pulmonary actinomycosis presenting with empyema

Allan,

Said,

Shanks

2023

BMJ Case Rep

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“…Notwithstanding, a recent study detected Actinomyces israelii's DNA in radicular cysts that did not present the actinomycotic colonies in histological sections (18). Whether Actinomyces DNA detection in periapical lesions can be secondary or a determinant factor for the infection is still unclear.…”

Section: Discussionmentioning

confidence: 95%

Clinicopathological and ultrastructural characterization of periapical actinomycosis

Gomes-Silva¹,

Pereira²,

Fregnani³

et al. 2020

Med Oral

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Background: The aim of the present study was to analyze the clinicopathological and the ultrastructural features of periapical actinomycosis (PA) cases. Material and Methods: Data from the files of an oral pathology laboratory were retrieved and the findings of histopathological analysis were evaluated. Hematoxylin-eosin (HE), a modified Brown & Brenn, and Grocott stains as well as ultrastructural analysis using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) were utilized. Results: Six cases were obtained, 4 females and 2 males, with a mean age of 34 year-old. Two cases were symptomatic, lower teeth and the anterior region were more commonly affected, and all cases were characterized by periapical radiolucencies. All cases presented sulfur granules with a ray-fungus or club-shaped pattern of the Splendore-Hoeppli phenomenon in HE-stained sections, with filamentous gram-positive bacteria aggregates highlighted by the modified Brown & Brenn stain. SEM analysis revealed abundant packed rod-like and filamentous bacteria associated with an extracellular amorphous material. EDX analysis showed predominant picks of calcium and sulfur in actinomycotic colonies. Conclusions: Our findings suggest that PA manifests either clinically and radiologically as a non-specific and heterogeneous condition and that the actinomycotic colonies consist in a calcium-and sulfur-rich matrix. Furthermore, the results highlight the importance of submitting periapical specimens after surgical removal to histopathological analysis.

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Actinomyces israelii in radicular cysts: a molecular study

Cited by 8 publications

References 17 publications

Bacteria and virulence factors in periapical lesions associated with teeth following primary and secondary root canal treatment

Bacteria and virulence factors in periapical lesions associated with teeth following primary and secondary root canal treatment

Pulmonary actinomycosis presenting with empyema

Clinicopathological and ultrastructural characterization of periapical actinomycosis

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