Actinobacteria from Marine Environments: A Unique Source of Natural Products

Girão, Mariana; Ribeiro, Inês; Carvalho, Maria de Fátima

doi:10.1007/978-981-16-6132-7_1

Cited by 7 publications

(8 citation statements)

References 344 publications

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“…Members of this phylum can be found in sundry environments, including marine ecosystems where they can establish symbiotic interactions with other organisms, as macroalgae (Gir ão et al, 2019). One of the most recognizable traits of these microorganisms is their unrivalled ability to synthesize biotechnologically relevant natural products (NP) (Gir ão et al, 2022;van Bergeijk et al, 2020), making the exploration of their metabolism for chemical novelty the driving force of many research programs. This biosynthetic capability is especially noteworthy for a more restricted group of Actinomycetota members, usually designated as actinomycetes.…”

Section: Introductionmentioning

confidence: 99%

Unveiling the culturable and non‐culturable actinobacterial diversity in two macroalgae species from the northern Portuguese coast

Girão,

Alexandrino,

Cao

et al. 2024

Environmental Microbiology

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Actinomycetota, associated with macroalgae, remains one of the least explored marine niches. The secondary metabolism of Actinomycetota, the primary microbial source of compounds relevant to biotechnology, continues to drive research into the distribution, dynamics, and metabolome of these microorganisms. In this study, we employed a combination of traditional cultivation and metagenomic analysis to investigate the diversity of Actinomycetota in two native macroalgae species from the Portuguese coast. We obtained and taxonomically identified a collection of 380 strains, which were distributed across 12 orders, 15 families, and 25 genera affiliated with the Actinomycetia class, with Streptomyces making up approximately 60% of the composition. Metagenomic results revealed the presence of Actinomycetota in both Chondrus crispus and Codium tomentosum datasets, with relative abundances of 11% and 2%, respectively. This approach identified 12 orders, 16 families, and 17 genera affiliated with Actinomycetota, with minimal overlap with the cultivation results. Acidimicrobiales emerged as the dominant actinobacterial order in both macroalgae, although no strain affiliated with this taxonomic group was successfully isolated. Our findings suggest that macroalgae represent a hotspot for Actinomycetota. The synergistic use of both culture‐dependent and independent approaches proved beneficial, enabling the identification and recovery of not only abundant but also rare taxonomic members.

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Section: Introductionmentioning

confidence: 99%

Unveiling the culturable and non‐culturable actinobacterial diversity in two macroalgae species from the northern Portuguese coast

Girão,

Alexandrino,

Cao

et al. 2024

Environmental Microbiology

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“…The widespread occurrence of prodigiosins in phylogenetically distant microbes suggests that these molecules may play a significant, albeit uncertain and yet to be fully defined, physiological role. In recent years, marine-sourced Actinomycetota have proved their value as source of relevant chemistry ( Girão et al, 2022 ). Some known prodigiosins and related molecules, exhibiting a wide range of bioactive properties, have been found to be produced by Streptomyces species living in association with sponges ( El-Bondkly et al, 2012 ; Abdelfattah et al, 2019 ) and inhabiting deep-sea sediments ( Song et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Decylprodigiosin: a new member of the prodigiosin family isolated from a seaweed-associated Streptomyces

Girão,

Freitas,

Martins

et al. 2024

Front. Pharmacol.

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Bioprospecting actinobacterial secondary metabolism from untapped marine sources may lead to the discovery of biotechnologically-relevant compounds. While studying the diversity and bioactive potential of Actinomycetota associated with Codium tomentosum, a green seaweed collected in the northern Portuguese cost, strain CT-F61, identified as Streptomyces violaceoruber, was isolated. Its extracts displayed a strong anticancer activity on breast carcinoma T-47D and colorectal carcinoma HCT116 cells, being effective as well against a panel of human and fish pathogenic bacteria. Following a bioactivity-guided isolation pipeline, a new analogue of the red-pigmented family of the antibiotics prodigiosins, decylprodigiosin (1), was identified and chemically characterized. Despite this family of natural products being well-known for a long time, we report a new analogue and the first evidence for prodigiosins being produced by a seaweed-associated actinomycete.

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“…Marine Actinomycetota have gathered attention due to their ability to synthesize molecules with unique chemical scaffolds, shaped by the exclusive natural pressures of their surroundings [ 22 ]. While some exist as free-living entities, others establish symbiotic relationships with several organisms, such as macroalgae; these associations prompt the synthesis of NPs with tailored bioactive properties [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Cellulamides: A New Family of Marine-Sourced Linear Peptides from the Underexplored Cellulosimicrobium Genus

Girão,

Murillo-Alba,

Martín

et al. 2024

Marine Drugs

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Bioprospecting the secondary metabolism of underexplored Actinomycetota taxa is a prolific route to uncover novel chemistry. In this work, we report the isolation, structure elucidation, and bioactivity screening of cellulamides A and B (1 and 2), two novel linear peptides obtained from the culture of the macroalga-associated Cellulosimicrobium funkei CT-R177. The host of this microorganism, the Chlorophyta Codium tomentosum, was collected in the northern Portuguese coast and, in the scope of a bioprospecting study focused on its associated actinobacterial community, strain CT-R177 was isolated, taxonomically identified, and screened for the production of antimicrobial and anticancer compounds. Dereplication of a crude extract of this strain using LC-HRMS(/MS) analysis unveiled a putative novel natural product, cellulamide A (1), that was isolated following mass spectrometry-guided fractionation. An additional analog, cellulamide B (2) was obtained during the chromatographic process and chemically characterized. The chemical structures of the novel linear peptides, including their absolute configurations, were elucidated using a combination of HRMS, 1D/2D NMR spectroscopy, and Marfey’s analysis. Cellulamide A (1) was subjected to a set of bioactivity screenings, but no significant biological activity was observed. The cellulamides represent the first family of natural products reported from the Actinomycetota genus Cellulosimicrobium, showcasing not only the potential of less-explored taxa but also of host-associated marine strains for novel chemistry discovery.

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Actinobacteria from Marine Environments: A Unique Source of Natural Products

Cited by 7 publications

References 344 publications

Unveiling the culturable and non‐culturable actinobacterial diversity in two macroalgae species from the northern Portuguese coast

Unveiling the culturable and non‐culturable actinobacterial diversity in two macroalgae species from the northern Portuguese coast

Decylprodigiosin: a new member of the prodigiosin family isolated from a seaweed-associated Streptomyces

Cellulamides: A New Family of Marine-Sourced Linear Peptides from the Underexplored Cellulosimicrobium Genus

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