Actinobacteria Associated with Vineyard Soils of Algeria: Classification, Antifungal Potential Against Grapevine Trunk Pathogens and Plant Growth-Promoting Features

Laassami, Affaf; Yekkour, Amine; Meklat, Atika; Djemouai, Nadjette; Zitouni, Abdelghani; Mokrane, Salim; Lecomte, Pascal; Rey, Patrice; Berraf-Tebbal, Akila

doi:10.1007/s00284-020-02097-x

Cited by 14 publications

(13 citation statements)

References 42 publications

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“…However, since bacteria can produce other molecules such as indole pyruvic acid, and indoleacetamid which can also react with the Salkowski reagent to result in a pink colour [61], confirmation of IAA production by these actinobacterial isolates using HPLC is required. The results showed that overall, the PCP traits assayed for in this study were widespread in the endophytic actinobacteria associated with grapevines being similar to other studies for actinobacteria recovered from grapevine rhizosphere soil [47], as endophytes of Camellia spp. [46], P. colorata [18] and Rhynchotoechum ellipticum [33].…”

Section: Discussionsupporting

confidence: 88%

“…This study employed similar isolation techniques and selective media used in other studies [33,38,48] so this is unlikely to be the reason for failure to isolate more diverse genera. In the current study actinobacteria colonies were recovered into pure culture after 14 days incubation, in contrast much longer incubation periods of up to 4 weeks were used by other researchers [34,47,48], with Kaewkla and Franco [45] incubating the isolation plates for up to 16 weeks. This may have resulted in the recovery of slower growing genera.…”

Section: Discussionmentioning

confidence: 98%

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Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines

et al. 2022

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Grapevine trunk diseases (GTDs) are a significant problem for New Zealand viticulture. Endophytic actinobacteria are of interest as potential biocontrol agents due to their ability to inhibit plant pathogens and improve plant growth. However, no studies have investigated the diversity of actinobacteria associated with grapevines in New Zealand vineyards and their bioactivity. Actinobacteria diversity in different ‘Sauvignon blanc’ vine tissues from three vineyards (conventional and organic management, and different vine ages) was assessed using different methods and media. Forty-six endophytic actinobacteria were isolated, with more isolates recovered from roots (n = 45) than leaves (n = 1) and shoot internodes (n = 0). More isolates were recovered from the organic (n = 21) than conventional (n = 8) vineyard, mature (25-year old; n = 21) than young (2-year old; n = 2) vines and using a tissue maceration technique (n = 40). Actinomycete Isolation Agar, International Streptomyces Project 2, and Starch Casein media were effective for actinobacteria isolation. Most of the isolates recovered belonged to Streptomyces, with one isolate identified as Mycolicibacterium. Forty isolates were assessed for antifungal activity and plant growth-promoting (PGP) characteristics. Of these, 13 isolates had antifungal activity against test GTD pathogens (Dactylonectria macrodidyma, Eutypa lata, Ilyonectria liriodendri, Neofusicoccum parvum, and N. luteum). Eighteen isolates exhibited more than one PGP trait; 25siderophore production (n = 25), phosphate solubilization (n = 6), and indole acetic acid production (n = 16). Two strains, Streptomyces sp. LUVPK-22 and Streptomyces sp. LUVPK-30, exhibited the best antifungal and PGP properties. This study revealed the diversity of culturable endophytic actinobacteria from grapevines in New Zealand vineyards and their biocontrol potential against GTD pathogens.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 98%

Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines

et al. 2022

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“…Actinobacteria is the second largest group of bacterial species in the soil samples. Most of the bacteria in this phylum can produce indole acetic acid and ammonia, which can also fix N 2 , all these properties of actinobacteria would promote tobacco growth ( Laassami et al, 2020 ). For the fungal community, unclassified_k_fungi with strong cellulose dissolution ability was present in the samples ( Song et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Differences in microbial diversity and environmental factors in ploughing-treated tobacco soil

Zhang

Shen

et al. 2022

Front. Microbiol.

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During agricultural production, plowing affects the existing traits of the planted soil, including environmental factors (physicochemical properties and soil enzymatic activity) and microbial community, but whether deep tillage and conventional tillage cause differences in soil microecology are unknown. In this study, the 16S rRNA high-throughput sequencing technology was combined with soil environmental factor detection to analyze the differences in microbial diversity of smokey soils at different depths. As a result, the composition and structure of microbial community varied in different soil depth after plowing. Two dominant phyla, Actinobacteria and Acidobacteria, have varied a lot between the deep-plowing treatment HS3 (the sample in 10–20 cm depth after deep-plowing treatment) sample and the conventional tillage HC3 (treatment the sample in 10–20 cm depth after conventional tillage) sample. The abundance of Actinobacteria has increased significantly, while the abundance of Acidobacteria has decreased significantly. Moreover, deep tillage increased the activity of sucrase (S-SC) and nitrate reductase (NR) in samples with soil depth below 20 cm. In summary, deep tillage disturbed spatial microbial diversity and environmental factors significantly. This would provide new guidance for improving farmland management strategies, optimizing the activation methods of soil layers, further improving crop planting soil, and increasing crop yield.

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“…Screening based on antagonistic and plant-growth-promotion abilities of certain strains-Streptomyces, Saccharothrix, Nocardia, Nocardiopsis, Actinoplanes, Lentzea, Promicromonospora, Nonomuraea, Saccharopolyspora, and Streptosporangium-showed an appreciable antagonistic activity against both Paeomoniella chlamydospora and Phaeoacremonium minimum. These strains were able to produce siderophores, ammonia, indole acetic acid, ACC deaminase, cellulase, and amylase, as well as to fix N 2 [218].…”

Section: Trichoderma Sppmentioning

confidence: 99%

Biostimulants in Viticulture: A Sustainable Approach against Biotic and Abiotic Stresses

Cataldo

Fucile

Mattii

2022

Plants

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Climate change and disproportionate anthropogenic interventions, such as the excess of phytopharmaceutical products and continuous soil tillage, are jeopardizing viticulture by subjecting plants to continuous abiotic stress. One of the main physiological repercussions of abiotic stress is represented by the unbalanced redox homeostasis due to the overproduction of reactive oxygen species (ROS), ultimately leading to a state of oxidative stress (detrimental to grape quality). To these are added the direct and indirect damages caused by pathogens (biotic stresses). In light of this scenario, it is inevitable that sustainable techniques and sensitivity approaches for environmental and human health have to be applied in viticulture. Sustainable viticulture can only be made with the aid of sustainable products. Biostimulant (PB) applications (including resistance inducers or elicitors) in the vineyard have become interesting maneuvers for counteracting vine diseases and improving grape quality. These also represent a partial alternative to soil fertilization by improving nutrient absorption and avoiding its leaching into the groundwater. Their role as elicitors has important repercussions in the stimulation of the phenylpropanoid pathway by triggering the activation of several enzymes, such as polyphenol oxidase, lipoxygenase, phenylalanine ammonia-lyase, and peroxidase (with the accumulation of phenolic compounds). The present review paper summarizes the PBs’ implications in viticulture, gathering historical, functional, and applicative information. This work aims to highlight the innumerable beneficial effects on vines brought by these products. It also serves to spur the scientific community to a greater contribution in investigating the response mechanisms of the plant to positive inductions.

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Actinobacteria Associated with Vineyard Soils of Algeria: Classification, Antifungal Potential Against Grapevine Trunk Pathogens and Plant Growth-Promoting Features

Cited by 14 publications

References 42 publications

Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines

Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines

Differences in microbial diversity and environmental factors in ploughing-treated tobacco soil

Biostimulants in Viticulture: A Sustainable Approach against Biotic and Abiotic Stresses

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