Actinobacillus pleuropneumoniae hlyX gene homology with the fnr gene of Escherichia coli

MacInnes, Janet I.; Kim, J E; Lian, C J; Soltes, Glenn

doi:10.1128/jb.172.8.4587-4592.1990

Cited by 57 publications

(49 citation statements)

References 37 publications

(13 reference statements)

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“…3). (43,62), and 28% identity to E. coli Fnr (47,57) and A. pleuropneumoniae HlyX (29). The highest homologies among these proteins were found in the helix-turn-helix motif in the C-terminal region (Fig.…”

Section: Resultsmentioning

confidence: 85%

“…4), as well as in the ORF240 (FnrN) protein of R. leguminosarum (6), whereas the N-terminal regions of the R. meliloti and A. caulinodans FixK proteins contained no cysteine residues. In A. pleuropneumoniae HlyX and P. aeruginosa Anr, the spacing between the cysteine residues was the same as in the Fnr protein (29,43,62). Expression of B. japonicum fixK is controlled by the FixlJ system.…”

Section: Resultsmentioning

confidence: 99%

“…In Actinobacillus pleuropneumoniae, the HlyX protein was identified as a probable regulator of hemolysin synthesis (29). The Pseudomonas aeruginosa Anr protein is a regulator required for induction of nitrate reduc-the symbiont of alfalfa, as part of a complex regulatory cascade leading to the expression of genes (nif and fix) that are required for a nitrogen-fixing symbiosis (3).…”

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confidence: 99%

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Characterization of a fixLJ-regulated Bradyrhizobium japonicum gene sharing similarity with the Escherichia coli fnr and Rhizobium meliloti fixK genes

1992

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We describe the cloning, sequencing, regulation, and mutational analysis of a Bradyrhizobium japonicum fixK-like gene whose product belongs to the family of Fnr-Crp-related regulatory proteins. The predicted 237-amino-acid FixK protein was found to share between 28 and 38% sequence identity with the Escherichia coli Fnr protein, other bacterial Fnr-like proteins (FnrN, Anr, and HlyX), and two rhizobial FixK proteins.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Characterization of a fixLJ-regulated Bradyrhizobium japonicum gene sharing similarity with the Escherichia coli fnr and Rhizobium meliloti fixK genes

1992

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“…Expression of a heterologous fnr gene (the hlyX gene of Actinobacillus pleuropneumoniae) confers a haemolytic phenotype upon E. coli K-12 (Lian et al, 1989;Green et al, 1992). The primary structure of HlyX is 73% identical to that of FNR (MacInnes et al, 1990). Like FNR, HlyX senses and responds to oxygen through the assembly/ disassembly of a [4Fe-4S] 2þ cluster and they recognize the same DNA target (Green and Baldwin, 1997a).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of Escherichia coli yfiD expression by FNR occupying a site at −93.5 involves the AR1‐containing face of FNR

Green

Baldwin

Richardson

1998

Molecular Microbiology

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SummaryThe promoter of the FNR-activated yfiD gene of Escherichia coli has an unusual architecture because it contains two FNR sites, an arrangement usually associated with FNR-mediated repression. Investigation of yfiD promoter derivatives with altered FNR sites revealed that occupation of the far upstream FNR site (FNR II) downregulated expression, despite the presence of a FNR dimer activating expression from the promoter proximal site (FNR I). Transcript mapping by primer extension, and mutagenesis of potential ¹10 elements, indicated that yfiD expression is driven from a single FNR-dependent promoter with FNR sites at ¹40.5 (FNR I) and ¹93.5 (FNR II). However, yfiD mRNA is processed in stationary-phase cultures independently of rne, rpoS, ihfA and fis to yield transcripts lacking 12 and 21 bases from their respective 5Ј ends. Single amino acid substitutions (G74→C, F92→S, A95→P, R184→P, P188→A or L193→P) in the surface of FNR that contains activating region 1 (AR1 contacts the ␣-subunit of RNA polymerase to promote transcription activation) reduced the inhibitor y effect of FNR at FNR II, indicating that this region of the protein may have a role in repression as well as activation. The FNR variant F92→S was notable because, although it activated transcription of yfiD (two FNR sites), it was unable to activate transcription from model Class I and II promoters, which contain only a single FNR site.

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“…For example, Escherichia coli Fnr (fumarate-nitrate reduction regulator) mediates global changes in gene expression during transitions between aerobic and anaerobic growth (12). Fnr homologs have been described for a number of bacterial species (7,16,22,29), including Shewanella oneidensis MR-1 (20) (formerly Shewanella putrefaciens MR-1 [28]), a facultatively anaerobic metal-reducing bacterium (19). The predicted S. oneidensis EtrA (electron transport regulator) protein (20) shares a high degree of amino acid sequence identity with E. coli Fnr and with the analogous Anr (anaerobic regulator of arginine deiminase and nitrate reductase) protein from Pseudomonas aeruginosa (50.8 and 73.6% identity, respectively), thus suggesting the possibility that etrA is involved in regulating anaerobic energy metabolism in MR-1 (20).…”

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Microarray Transcription Profiling of a Shewanella oneidensis etrA Mutant

et al. 2002

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DNA microarrays were used to examine the effect of an insertional mutation in the Shewanella oneidensis etrA (electron transport regulator) locus on gene expression under anaerobic conditions. The mRNA levels of 69 genes with documented functions in energy and carbon metabolism, regulation, transport, and other cellular processes displayed significant alterations in transcript abundance in an etrA-mutant genetic background. This is the first microarray study indicating a possible involvement of EtrA in the regulation of gene expression in S. oneidensis MR-1

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Actinobacillus pleuropneumoniae hlyX gene homology with the fnr gene of Escherichia coli

Cited by 57 publications

References 37 publications

Characterization of a fixLJ-regulated Bradyrhizobium japonicum gene sharing similarity with the Escherichia coli fnr and Rhizobium meliloti fixK genes

Characterization of a fixLJ-regulated Bradyrhizobium japonicum gene sharing similarity with the Escherichia coli fnr and Rhizobium meliloti fixK genes

Downregulation of Escherichia coli yfiD expression by FNR occupying a site at −93.5 involves the AR1‐containing face of FNR

Microarray Transcription Profiling of a Shewanella oneidensis etrA Mutant

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