Actinidia deliciosa in vitro II. Growth and exogenous carbohydrates utilization by explants

Mezzetti, Bruno; Purcaro, Giorgia; Rosati, P.

doi:10.1007/bf00039937

Cited by 13 publications

(1 citation statement)

References 18 publications

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“…Îêð³ì ì³íåðàëüíîãî, íà ð³ñò ðîñëèí àêòè-í³ä³¿ âïëèâàº ïîâ³òðÿíå aeèâëåííÿ, òîáòî ôîòîñèíòåç, àáî âèêîðèñòàííÿ äaeåðåë ãåòåðîòðîôíîãî aeèâëåííÿ. Çà äàíèìè [34], óíàñë³äîê ïîãëèíàííÿ A. deliciosa ñèíòåòè÷íèõ âóãëåâîä³â ³ç ïîaeèâíîãî ñåðåäîâèùà âèõ³äíà ê³ëüê³ñòü ñàõàðîçè ï³ñëÿ 15 ä³á êóëüòèâóâàííÿ çíèçèëàñÿ äî 32%, ÷åðåç 30 ä³á -äî 4% ³ äî 0,08% íàïðèê³íö³ ïåð³îäó êóëüòèâóâàííÿ (60 ä³á). Çá³ëüøåííÿ ê³ëüêîñò³ ñàõàðîçè â åêñïëàíòàòàõ ïàðàëåëüíî ç ¿¿ çìåíøåííÿì ó ñåðåäîâèù³ íå â³äáóâàëîñÿ.…”

Section: ðåçóëüòàòè äîñë³äAeåíüunclassified

Micropropagation of plants of the genus Actinidia Lindl.

Kyienko

Kimeichuk

Matskevych

2022

PVSandP

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Purpose. Analysis of plant micropropagation technologies for the creation of viable interspecific hybrids and varieties of Actinidia Lindl. Methods. General scientific – hypothesis, experiment, observation, analysis, synthesis method for drawing conclusions. Results. The introduction of in vitro technologies is now becoming the dominant commercial method of large-scale and rapid production of seedlings with stable inheritance of variety traits, high multiplication rate, preservation of economically valuable traits in the absence of production seasonality and time constraints. In addition to reproduction, the breeding process is also accelerated, including mutagenesis and hybridization. It is important to obtain not only a sterile explant, but also a morphogenically active one, that is, a plant that takes roots and subsequently regenerates in vitro. The best in terms of decontamination efficiency is the method of treatment with hypochlorite and the addition of PPM biocide to the nutrient medium, but under these conditions, the lowest survival of explants in all samples was noted. The efficiency of introduction into aseptic culture at the first stage of micropropagation is also affected by the biological characteristics of the primary explants. In studies with nutrient media for A. arguta, it was found that of the elements of mineral nutrition, only 11 ions are necessary for life: five macro- (N, K, P, Mg, S) and six microelements (Cl, Fe, B, Mo, Na, I). Plants in vitro have a lower dry matter content and a greater amount of moisture, including free moisture, which is quickly lost when the water balance is disturbed. Conclusions. The ability to regenerate is more pronounced in the species A. chinensis and A. deliciosa, and to a lesser extent in A. arguta. For A. chinensis, the use of hydroponic technology for the adaptation of regenerants at the ex vitro stage is effective.

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Section: ðåçóëüòàòè äîñë³äAeåíüunclassified

Micropropagation of plants of the genus Actinidia Lindl.

Kyienko

Kimeichuk

Matskevych

2022

PVSandP

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Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricot

Marino

Bertazza

Magnanini

et al. 1993

Plant Cell Tiss Organ Cult

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In vitro proliferation and rooting capacity of 'San Castrese ' and 'Portici' apricots (Prunus armeniaca L.) were tested on modified MS medium enriched with varying growth regulator concentrations and sucrose (58.4 mM) or sorbitol (116.8 mM) as main carbon energy sources. The interaction of proliferation and rooting media was also studied.Proliferation of both cultivars was proportional to benzyladenine (BA) concentration and enhanced with sorbitol media. However, 8.8 p~M BA was often associated with hyperhydricity, particularly when shoots were grown on sucrose media. Newly proliferated shoots elongated better on sorbitol media. The positive influence of sorbitol on proliferation and shoot growth was not due to osmotic effects. Moreover, sorbitol showed a positive carryover effect in hastening rooting of 'Portici'. By contrast, when transferred to sorbitol rooting media, the shoots of both cultivars generally showed low rooting, with short, thick roots.Up to 70% of the plantlets that produced roots in sucrose media enriched with indolebutyric acid were successfully acclimatized when they were dipped in a benomyl (0.075% w/v) suspension before being transplanted with care being taken to prevent over-wetting of soil.

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Micropropagation of Actinidia kolomikta

Kováč

1993

Plant Cell Tiss Organ Cult

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Actinidia deliciosa in vitro II. Growth and exogenous carbohydrates utilization by explants

Cited by 13 publications

References 18 publications

Micropropagation of plants of the genus Actinidia Lindl.

Micropropagation of plants of the genus Actinidia Lindl.

Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricot

Micropropagation of Actinidia kolomikta

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