Actin microfilaments are involved in the regulation of HVA1 transcript accumulation in drought-treated barley leaves

Śniegowska-Świerk, Katarzyna; Dubas, Ewa; Rapacz, Marcin

doi:10.1016/j.jplph.2016.02.006

Cited by 12 publications

(10 citation statements)

References 17 publications

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“…Amongst the selected genes related to the drought response, actin has been confirmed to play a role in drought responses. According to [23], barley genotypes tolerant to drought are characterized by a lower level of actin gene expression under drought conditions, which was confirmed by the currently obtained results. This lower level of expression may be associated with a higher drought susceptibility threshold.…”

Section: Discussionsupporting

confidence: 82%

Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

Wójcik-Jagła

Rapacz

Dubas

et al. 2020

IJMS

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Plant tolerance to environmental stress is determined by a very complicated network composed of many intra- and extracellular factors. The aim of this study was to select candidate genes involved in responses to freezing and drought in barley on the basis of previous proteomic studies and to analyze changes in their expression caused by application of both stress factors. Six candidate genes for freezing tolerance (namely the genes encoding elongation factor 1 alpha (EF1A), ferredoxin-NADP reductase, a 14-3-3a protein, β-fructofuranosidase, CBF2A and CBF4B) and six for drought tolerance (encoding transketolase, periplasmic serine protease, triosephosphate isomerase, a protein with a co-chaperon region (GroEs), pfam14200 and actin) were chosen arbitrarily on the basis of in silico bioinformatic analyses. The expression levels of these genes were measured under control and stress conditions in six DH (doubled haploid) lines with differing freezing and drought tolerance. The results of gene expression analysis confirmed the roles of the candidate genes preselected in this study on the basis of previous proteome analysis in contributing to the differences in freezing and drought tolerance observed in the studied population of DH lines of winter barley.

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Section: Discussionsupporting

confidence: 82%

Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

Wójcik-Jagła

Rapacz

Dubas

et al. 2020

IJMS

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“…Previous studies of AFs in CamB and Maresi genotypes showed that these genotypes differ in actin content and AF organization, both in optimal water conditions and after leaf desiccation (Sniegowska-Swierk et al, 2015 ). This observation was accompanied with an increase of actin 11 ( ACT11 ) expression in leaves of Maresi and actin depolymerizing factor 3 ( ADF3 ) expression in leaves of both genotypes (Sniegowska-Swierk et al, 2016 ). Our transcriptome analysis is consistent with these previous data, showing an increase in the expression of ADF3 gene in both genotypes and both organs under drought stress.…”

Section: Discussionmentioning

confidence: 98%

“…It has been suggested that actin filaments (AFs) may participate in drought tolerance, as they form a network connecting cell walls, plasma membranes and cellular compartments. Through this physical connections they may act as osmotic sensors, because a decrease of turgor changes the compression of AFs, what in turn may be recognized by the cell as a signal to switch on expression of drought-responsive genes (Huang et al, 2012 ; Sniegowska-Swierk et al, 2015 , 2016 ). Previous studies of AFs in CamB and Maresi genotypes showed that these genotypes differ in actin content and AF organization, both in optimal water conditions and after leaf desiccation (Sniegowska-Swierk et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

No Time to Waste: Transcriptome Study Reveals that Drought Tolerance in Barley May Be Attributed to Stressed-Like Expression Patterns that Exist before the Occurrence of Stress

et al. 2018

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Plant survival in adverse environmental conditions requires a substantial change in the metabolism, which is reflected by the extensive transcriptome rebuilding upon the occurrence of the stress. Therefore, transcriptomic studies offer an insight into the mechanisms of plant stress responses. Here, we present the results of global gene expression profiling of roots and leaves of two barley genotypes with contrasting ability to cope with drought stress. Our analysis suggests that drought tolerance results from a certain level of transcription of stress-influenced genes that is present even before the onset of drought. Genes that predispose the plant to better drought survival play a role in the regulatory network of gene expression, including several transcription factors, translation regulators and structural components of ribosomes. An important group of genes is involved in signaling mechanisms, with significant contribution of hormone signaling pathways and an interplay between ABA, auxin, ethylene and brassinosteroid homeostasis. Signal transduction in a drought tolerant genotype may be more efficient through the expression of genes required for environmental sensing that are active already during normal water availability and are related to actin filaments and LIM domain proteins, which may function as osmotic biosensors. Better survival of drought may also be attributed to more effective processes of energy generation and more efficient chloroplasts biogenesis. Interestingly, our data suggest that several genes involved in a photosynthesis process are required for the establishment of effective drought response not only in leaves, but also in roots of barley. Thus, we propose a hypothesis that root plastids may turn into the anti-oxidative centers protecting root macromolecules from oxidative damage during drought stress. Specific genes and their potential role in building up a drought-tolerant barley phenotype is extensively discussed with special emphasis on processes that take place in barley roots. When possible, the interconnections between particular factors are emphasized to draw a broader picture of the molecular mechanisms of drought tolerance in barley.

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“…On the other hand, photosynthetic capacity in drought was clearly related to the expression level of the dehydrine encoding gene HVA1 (Rapacz et al, 2010; Wójcik-Jagła et al, 2012). This complex relationship was probably connected to the proposed mechanism of the induction of HVA1 expression in drought, which is triggered by intact changes of turgor in mesophyll cells sensed by the actin cytoskeleton (Wójcik-Jagła et al, 2012; Sniegowska-Swierk et al, 2015, 2016). To summarize, these studies showed that in barley, high photosynthetic activity can be maintained during moderate drought if the plants effectively protect the cells from dehydration irrespective of maintaining high turgidity at the cost of closing the stomata.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Associations of Chlorophyll Fluorescence OJIP Transient Parameters Connected With Soil Drought Response in Barley

et al. 2019

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One hundred and nine accessions of spring barley seedlings were phenotyped under soil drought conditions. Chlorophyll fluorescence induction (OJIP) parameters, leaf water content, relative turgidity, net assimilation rate ( P N ), and water use efficiency (WUE) of plants were measured. All the tested lines were genotyped by means of DArT sequencing (DArTseq) technology. For association mapping a 11,780 polymorphic DArTseq and 4,725 DArTseq SNP markers were used. Our results revealed dissimilar patterns of the relationships between OJIP-parameters under control and drought conditions. A high level of correlation between parameters characterizing Photosystem's II (PSII) energy trapping efficiency (F v /F m ) and photochemical events downstream of PSII reaction center (e.g., Performance Index—PI CSo ) was observed only in the case of drought-treated plants. Generally, OJIP parameters were correlated with leaf water content (less in control). This correlation was weaker with WUE, and absent with P N . Under drought stress, 6,252 genotype × phenotype associations, which passed false discovery rate (FDR) verification, were found between all the studied phenotypic characteristics (23, including 19 OJIP parameters) and 2,721 markers. On the other hand, only 282 associations passed FDR test in the control. They comprised 22 phenotypic parameters and 205 markers. Probing for gene annotations of sequences was performed for markers associated with F v /F m for both drought and control, markers were associated with studied traits in both control and drought, as well as for markers associated with both OJIP and other physiological parameters in drought. Our work allowed us to conclude that drought treatment differentiates the studied lines through the revealing of relationships between water content and the damages to PSII reaction centers or different components of PSII energy transfer chain. Moreover, the former was not connected with net photosynthesis rate.

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Actin microfilaments are involved in the regulation of HVA1 transcript accumulation in drought-treated barley leaves

Cited by 12 publications

References 17 publications

Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

No Time to Waste: Transcriptome Study Reveals that Drought Tolerance in Barley May Be Attributed to Stressed-Like Expression Patterns that Exist before the Occurrence of Stress

Genome-Wide Associations of Chlorophyll Fluorescence OJIP Transient Parameters Connected With Soil Drought Response in Barley

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