Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II

Hofmann, Wilma A.; Stojiljković, Ljuba; Fuchsová, Beata; Vargas, Gabriela M.; Mavrommatis, Evangelos; Philimonenko, Vlada; Kyselá, Katarína; Goodrich, James A.; Lessard, James L.; Hope, Thomas J.; Hozák, Pavel; Lanerolle, Primal de

doi:10.1038/ncb1182

Cited by 340 publications

(316 citation statements)

References 38 publications

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“…That indicated that expression of certain target genes were regulated because b-actin is a co-factor of RNA polymerase II in gene transcription [25,26]. Actin is abundant in the nucleus and has been implicated in transcription [25] and RNA polymerase II is present in the nucleolus-like body at all transcriptional states of the oocyte nucleus [27].…”

Section: Discussionmentioning

confidence: 99%

“…Actin is abundant in the nucleus and has been implicated in transcription [25] and RNA polymerase II is present in the nucleolus-like body at all transcriptional states of the oocyte nucleus [27]. Actin and RNA polymerase II co-localize in nucleus [25,28]. Possibly when the expression of some genes, such as ICAM-1 and integrin b1 is changed, the b-actin and RNA polymerase II are recruited and transferred to nucleolus.…”

Section: Discussionmentioning

confidence: 99%

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Lipoprotein p37 from Mycoplasma hyorhinis inhibiting mammalian cell adhesion

et al. 2005

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Summaryp37 protein is a membrane lipoprotein of Mycoplasma hyorhinis, and our previous work showed that there was high ratio of M. hyorhinis infection in human gastric carcinoma. To investigate the possible functions of p37 in cancer development, the nucleotide sequence of p37 gene was modified and expressed well in transfected cells. We found that p37 localized at the Golgi apparatus and could be secreted out of the cell. Human gastric cancer cells AGS, after being transfected with the p37 gene, were smaller, more spherical and easy to detach from each other. Their adhesion to matrix was also diminished and cytoskeleton in these stable p37 AGS cell was rearranged and transcription co-factor b-actin was transferred to nucleolus with down-regulation of ICAM-1 and integrin b1. These findings will be helpful for us to elucidate the effects of p37 on eukaryotic cells as well as to better understand the potential relationship between cancer and mycoplasma infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Lipoprotein p37 from Mycoplasma hyorhinis inhibiting mammalian cell adhesion

et al. 2005

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“…3 Several studies have provided evidence that nuclear actin associates with three types of nuclear complexes involved in gene expression: chromatin remodeling complexes, [10][11][12] ribonucleoprotein particles 13,14 and the three RNA polymerases. [15][16][17] CapG as a nuclear actin-binding protein could play a role in preventing the nuclear actin from polymerizing and keeping it in a monomeric globular or a short oligomeric form. Thus, it could have some indirect influence on the regulation of gene expression.…”

mentioning

confidence: 99%

Invasive breast cancer cells exhibit increased mobility of the actin‐binding protein CapG

Renz

Betz

Niederacher

et al. 2008

Intl Journal of Cancer

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The CapG protein, a Gelsolin-related actin-binding protein, is expressed at higher levels in breast cancer, especially in metastasizing breast cancer, than in normal breast epithelium. Furthermore, it is known that an increased expression of the CapG protein triggers an increase in cell motility. According to in vitro experiments, it was supposed that it is the nuclear fraction of the protein, which causes the increase in cell motility. Here, we examined the dynamical distribution of the CapG protein within the living cell, i.e. the import of the CapG protein into the nucleus. The nuclear import kinetics of invasive, metastasizing breast cancer cells were compared to the import kinetics of non-neoplastic cells similar to normal breast epithelium. FRAP kinetics showed a highly significant increase in the recovery of photobleached CapG-eGFP in the cancer cells, so that a differentiation of invasive, metastasizing cells and non-invasive, non-metastasizing cells on the basis of transport processes of the CapG protein between the nucleus and the cytoplasm seems to be possible. Comprehension of the mobility and compartmentalization of the CapG protein in normal and in cancer cells in vivo could constitute a new basis to characterize the invasiveness and metastasizing potential of breast cancer. ' 2007 Wiley-Liss, Inc.

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“…Interestingly, the formation of actin rods implies not only an alteration of the synapses but it also contributes to an impairment of gene transcription. It has been reported that both nuclear actin and cofilin are part of the preinitiation complex and are implicated in the transcription of most RNA polymerase II genes, [85][86][87] directly connecting actin filaments to transcriptional regulation. When the actin-cofilin equilibrium is perturbed by oxidative stress and excitotoxicity, transcription is affected leading to gene repression.…”

Section: Multiple Targets For Gene Repression In the Nucleus Transglumentioning

confidence: 99%

Transglutaminase is a Therapeutic Target for Oxidative Stress, Excitotoxicity and Stroke: A new Epigenetic Kid on the Cns Block

Basso

Ratan

2013

J Cereb Blood Flow Metab

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Transglutaminases (TGs) are multifunctional, calcium-dependent enzymes that have been recently implicated in stroke pathophysiology. Classically, these enzymes are thought to participate in cell injury and death in chronic neurodegenerative conditions via their ability to catalyze covalent, nondegradable crosslinks between proteins or to incorporate polyamines into protein substrates. Accumulating lines of inquiry indicate that specific TG isoforms can shuttle into the nucleus when they sense pathologic changes in calcium or oxidative stress, bind to chromatin and thereby transduce these changes into transcriptional repression of genes involved in metabolic or oxidant adaptation. Here, we review the evidence that supports principally a role for one isoform of this family, TG2, in cell injury and death associated with hemorrhagic or ischemic stroke. We also outline an evolving model in which TG2 is a critical mediator between pathologic signaling and epigenetic modifications that lead to gene repression. Accordingly, the salutary effects of TG inhibitors in stroke may derive from their ability to restore homeostasis by removing inappropriate deactivation of adaptive genetic programs by oxidative stress or extrasynaptic glutamate receptor signaling.

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Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II

Cited by 340 publications

References 38 publications

Lipoprotein p37 from Mycoplasma hyorhinis inhibiting mammalian cell adhesion

Lipoprotein p37 from Mycoplasma hyorhinis inhibiting mammalian cell adhesion

Invasive breast cancer cells exhibit increased mobility of the actin‐binding protein CapG

Transglutaminase is a Therapeutic Target for Oxidative Stress, Excitotoxicity and Stroke: A new Epigenetic Kid on the Cns Block

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