Actin cytoskeleton dynamics in mammalian oocyte meiosis†

Duan, Xing; Sun, Shao‐Chen

doi:10.1093/biolre/ioy163

Cited by 100 publications

(61 citation statements)

References 120 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that absence or inhibition of actin filaments leads to abnormal spindle position (Miao et al ). Actin filaments are also essential for cytokinesis, oocyte without actin filaments in the cortex, the cytokinesis rings will not form, and eventually induced failure of the first polar body extrusion (Terada et al ; Duan and Sun ). A fundamental premise to extrude a normal polar body is that the spindle is positioned close to the cortex and actin filaments are normally distributed in the cortex (Kexi and Rong ).…”

Section: Discussionmentioning

confidence: 99%

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

Meng

Jiao

Chen

et al. 2020

Environ and Mol Mutagen

View full text Add to dashboard Cite

As a member of parabens (PBs), Isobutylparaben (IBP) has a broad-spectrum antimicrobial activity and widely used in personal care products and cosmetics. Recent studies have indicated that usage of IBP poses a potential threat to reproductive health. In this study, we aimed to reveal the effects of acute exposure to IBP on the meiotic maturation of porcine cumulus oocyte complexes. Initial study showed that 200 μM of IBP significantly reduced the rate of the first polar body extrusion with no significant effect on cumulus cell expansion; however, 400 μM of IBP could significantly affect both. Further research revealed that abnormal spindles, misalignment chromosomes, and aberrant distributed actin filaments were detected in IBP-treated oocytes, which indicates that the cytoskeleton architecture of oocyte could be the target of IBP. At the same time, ROS level and apoptosis rate of oocyte were significantly increased by IBP exposure. Moreover, the levels of H3K9me3 and H3K27me3 were significantly induced in oocytes by IBP. Collectively, these results demonstrate that acute exposure to IBP could disrupt porcine oocyte maturation through affecting cytoskeleton, oxidative stress, viability and epigenetic modification. Environ. Mol. Mutagen. 61:433-444, 2020. Fig. 7. Schematic representation of negative effects of IBP on porcine oocytes. Oocyte exposure to IBP during meiotic maturation leads to inhibition of cumulus cell expansion, disruption of cytoskeleton, generation of oxidative stress and apoptosis, and alteration of epigenetic modifications. Environmental and Molecular Mutagenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

Meng

Jiao

Chen

et al. 2020

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…Mammalian oocytes undergo two consecutive rounds of meiotic division and generate a large haploid egg and two small polar bodies [ 1 ]. The majority of the oocyte cytoplasm is retained during this process, which contains most of the organelles including mitochondria, providing sufficient storage materials and dynamophore (energy carrier) for early embryonic development after fertilization [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation

Zhang

Pan

et al. 2020

J Animal Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

Background Environmental pollution induces oxidative stress and apoptosis in mammalian oocytes, which can cause defects in reproduction; however, the molecular regulation of oxidative stress in oocytes is still largely unknown. In the present study, we identified that dynamin-related protein 1 (DRP1) is an important molecule regulating oocyte mitochondrial function and preventing oxidative stress/apoptosis. DRP1 is a member of the dynamin GTPase superfamily localized at the mitochondrial-endoplasmic reticulum interaction site, where it regulates the fission of mitochondria and other related cellular processes. Results Our results show that DRP1 was stably expressed during different stages of porcine oocyte meiosis, and might have a potential relationship with mitochondria as it exhibited similar localization. Loss of DRP1 activity caused failed porcine oocyte maturation and cumulus cell expansion, as well as defects in polar body extrusion. Further analysis indicated that a DRP1 deficiency caused mitochondrial dysfunction and induced oxidative stress, which was confirmed by increased reactive oxygen species levels. Moreover, the incidence of early apoptosis increased as detected by positive Annexin-V signaling. Conclusions Taken together, our results indicate that DRP1 is essential for porcine oocyte maturation and that a DRP1 deficiency could induce mitochondrial dysfunction, oxidative stress, and apoptosis.

show abstract

“…Figure 3b), and MAPK, PI3K-AKT, HIPPO and calcium signaling pathways (Supp. Figure 4a-d) have been shown previously to play important roles in oocyte structure and function [63][64][65][66][67][68][69] .…”

Section: Follicular Fluid Evs Protein Content and Their Possible Rolementioning

confidence: 76%

Follicular extracellular vesicles enhance meiotic resumption of domestic cat vitrified oocytes

Ferraz

Fujihara

Nagashima

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Extracellular vesicles (EVs) contain multiple factors that regulate cell and tissue function. However, understanding of their influence on gametes, including communication with the oocyte, remains limited. In the present study, we characterized the proteome of domestic cat (Felis catus) follicular fluid EVs (ffEV). To determine the influence of follicular fluid EVs on gamete cryosurvival and the ability to undergo in vitro maturation, cat oocytes were vitrified using the Cryotop method in the presence or absence of ffEV. Vitrified oocytes were thawed with or without ffEVs, assessed for survival, in vitro cultured for 26 hours and then evaluated for viability and meiotic status. Cat ffEVs had an average size of 129.3 ± 61.7 nm (mean ± SD) and characteristic doughnut shaped circular vesicles in transmission electron microscopy. Proteomic analyses of the ffEVs identified a total of 674 protein groups out of 1,974 proteins, which were classified as being involved in regulation of oxidative phosphorylation, extracellular matrix formation, oocyte meiosis, cholesterol metabolism, glycolysis/gluconeogenesis, and MAPK, PI3K-AKT, HIPPO and calcium signaling pathways. Furthermore, several chaperone proteins associated with the responses to osmotic and thermal stresses were also identified. There were no differences in the oocyte survival among fresh and vitrified oocyte; however, the addition of ffEVs to vitrification and/or thawing media enhanced the ability of frozen-thawed oocytes to resume meiosis. In summary, this study is the first to characterize protein content of cat ffEVs and their potential roles in sustaining meiotic competence of cryopreserved oocytes.

show abstract

Actin cytoskeleton dynamics in mammalian oocyte meiosis†

Cited by 100 publications

References 120 publications

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation

Follicular extracellular vesicles enhance meiotic resumption of domestic cat vitrified oocytes

Contact Info

Product

Resources

About