Actin-Binding Proteins and Disease

We report a drug dose-response, end-point study of intracellular filamentous actin (F-actin) by automated fluorescence microscopy, complemented with theoretical kinetic simulation of drug action. We highlight the use of an advanced orientation-sensitive image processing procedure ( transform), specially tailored for the detection of ordered filamentous "patches" in cell images. To examine the extent of stress F-actin disruption caused by the drug, we compare the measured response based on the above transformation with the theoretical data obtained from a quantitative model. We show that the assay data are consistent with the first-order mass action kinetics predicted by a basic reaction model. As a concluding remark, we briefly discuss advantages, perspectives, and challenges of conventional fluorescent microscopy within the context of the quantitative high-throughput screening paradigm.

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Diagnosing phenotypes of single-sample individuals by edge biomarkers

Zhang

Zeng

et al. 2015

J Mol Cell Biol

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Actin-Binding Proteins and Disease

Cited by 4 publications

References 962 publications

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Actin-Binding Toxin “Tail” Wags the Dog

Microscopy-Based HTS Examines the Mechanism of Stress F-Actin Fiber Disruption by Cytochalasin D: Orientation Texture Data Collated With Quantitative Kinetic Modeling

Diagnosing phenotypes of single-sample individuals by edge biomarkers

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