Actin, a reliable marker of internal control?

Ruan, Wenjie; Lai, Maode

doi:10.1016/j.cca.2007.07.003

Cited by 161 publications

(107 citation statements)

References 33 publications

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“…ACT as traditional reference genes is not always stable in different experimental conditions, which was somewhat in accordance with several studies demonstrating ACT as an unsuitable internal control for RT-PCR in other cell types or species (Lord et al 2010). Recent studies have shown that ACT expression can change in response to a variety of conditions (Ruan & Lai 2007;Zarivi et al 2015;Wrzesińska et al 2016). Thus, these traditionally used reference genes are not persistently and stably expressed in many species or different experimental treatments (Chandna 2012;Cheng et al 2013;Ma et al 2016), which emphasised the need to evaluate reference genes in A. fatua.…”

Section: Discussionsupporting

confidence: 86%

Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua

Liu¹,

Li²,

Lu³

et al. 2019

Plant Prot. Sci.

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Citation: Liu J., Li P., Lu L., Xie L., Chen X., Zhang B. (2019): Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua. Plant Protect. Sci., 55: 61-71.Abstract: Eight commonly used candidate reference genes, 18S ribosomal RNA (rRNA) (18S), 28S rRNA (28S), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1α), ribosomal protein L7 (RPL7), Alpha-tubulin (α-TUB), and TATA box binding protein-associated factor (TBP), were evaluated under various experimental conditions to assess their suitability in different developmental stages, tissues and herbicide treatments in Avena fatua. The results indicated the most suitable reference genes for the different experimental conditions. For developmental stages, 28S and EF1α were the optimal reference genes, both EF1α and 28S were suitable for experiments of different tissues, whereas for herbicide treatments, GAPDH and ACT were suitable for normalizations of expression data. In addition, GAPDH and EF1α were the suitable reference genes.

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Section: Discussionsupporting

confidence: 86%

Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua

Liu¹,

Li²,

Lu³

et al. 2019

Plant Prot. Sci.

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show abstract

“…The β-actin, a non-muscle cytoskeletal protein, has a relatively stable expression, therefore it is worldwide applied as a reference gene in the course of PCR [49]. Newer data indicated that β-actin is differentially expressed in the brain specimens of both AD and control subjects [23].…”

Section: Discussionmentioning

confidence: 99%

Increase in Alzheimer's related markers preceeds memory disturbances: Studies in vasopressin-deficient Brattleboro rat

Várga

Klausz

Domokos

et al. 2014

Brain Research Bulletin

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Alzheimer's disease (AD) is the most common form of dementia in the elderly. For more effective therapy early diagnostic markers could be beneficial. Therefore we compared one year old rats with adults and examined if changes in possible brain markers of AD preceeded memory decline. We also tested if vasopressin-deficient animals were useful model of AD as vasopressin has well known positive effect on memory and AD patient has decreased vasopressin production.We compared adult (3 month) and old (12 month), normal and vasopressin-deficient Brattleboro rats. To receive a comprehensive picture about their memory we examined their social discrimination, object discrimination and conditioned learning abilities (shuttle box).Amyloid precursor protein (APP), mitogen-activated protein kinase 1 (MAPK1), β-actin and tryptophan 2,3-dioxygenase 2 (TDO2) mRNA levels was measured by quantitative PCR.There was no difference between the memory of adult and aged groups. The vasopressin-deficient rats at both ages showed a weaker performance in the course of social and object discrimination tests and a higher escape failure during the shuttle box experiment. The brain marker mRNAs of the elder animals were higher than the levels of the adults, but the absence of vasopressin had no influence on them.Thus, the one year old rats showed elevated levels of AD-related markers, but memory deficits were observable only in vasopressin deficient animals. Vasopressin does not seem to have pathogenic role in AD. Changes in the studied markers might predict later symptoms, although further studies are required for confirmation.

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“…2, a9) and alphatubulin (Fig. 2, a10), both components of the cytoskeleton, are commonly used to normalize molecular expression studies because of their functions as endogenous housekeeping genes (Ruan and Lai 2007). In summary, these downregulated proteins participate in cell metabolism, structure, and stability and are expressed at a high level when the physiological status of cotton is normal.…”

Section: Proteins Related To Cell Deathmentioning

confidence: 99%

Proteomics-based analysis reveals that Verticillium dahliae toxin induces cell death by modifying the synthesis of host proteins

Xie

Wang

et al. 2013

J Gen Plant Pathol

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Verticillium dahliae is one of the most destructive soil-borne fungal pathogens that cause vascular wilt diseases in a wide range of important crop plants, including cotton. However, the mechanisms used by this pathogen to infect cotton have not been fully elucidated. In the present study, we first investigated changes in protein abundance during the initial interaction between cotton roots and V. dahliae. Among the proteins that were upregulated upon infection, some were related to reactive oxygen species (ROS); among those downregulated upon infection were proteins involved in normal metabolism or cell structure. Further experiments confirmed that a sudden release of ROS and cell death accompany V. dahliae infection in the cotton vasculature. Further analysis indicated that a culture supernatant of V. dahliae induced lesion formation in tobacco leaves; de novo protein synthesis not active gene expression was required for this induction. Lesion formation was dependent on the age of leaves, but neither the known ROS burst nor the ubiquitin/ 26S proteasome system are prerequisites.

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Actin, a reliable marker of internal control?

Cited by 161 publications

References 33 publications

Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua

Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua

Increase in Alzheimer's related markers preceeds memory disturbances: Studies in vasopressin-deficient Brattleboro rat

Proteomics-based analysis reveals that Verticillium dahliae toxin induces cell death by modifying the synthesis of host proteins

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