ACTH Secreting Transplantable Pituitary Tumors.

Fürth, J.; Gadsen, E. L.; Upton, Arthur C.

doi:10.3181/00379727-84-20607

Cited by 58 publications

(29 citation statements)

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“…3 Present address: Department of Medicine, Meir Hospital, Kfar-Saba, Israel. 4 Present address: Department of Medicine, University of Chicago, Pritzker School of Medicine, Chicago, Illinois 60637. 5 Abbreviations: ACTH = adrenocorticotropic hormone; MSH = melanocyte-stimulating hormone.…”

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confidence: 99%

ACTH and MSH Production by a Single Cloned Mouse Pituitary Tumor Cell Line

et al. 1973

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The production of ACTH and MSH was studied in the cloned, functional, murine pituitary tumor cell line, AtT-20. Extracts of normal whole LAFj mouse pituitaries, tumors induced by injecting AtT-20 cells into recipient LAF X mice, cultured AtT-20 cells, and the medium in which the cells were cultured were subjected to molecular sieve chromatography on Sephadex G-SO fine resin. The original extracts and the Sephadex fractions were assayed for bioactive ACTH and MSH. Each of the extracts contained both ACTH and MSH. The ACTH existed as two distinct moieties, one of which eluted earlier than the ACTH standard. The MSH eluted in later fractions and thus was a separate molecular entity from the ACTH's. The extracts and fractions were subjected to radioimmunoassays for ACTH, a-MSH, and P-MSH. Both ACTH moieties were immunologically indistinguishable from highly purified human ACTH standard. The MSH in normal mouse pituitary and AtT-20 tumor was immunologically indistinguishable from a-MSH standard, but less than one-fourth of the MSH biologic activity could be accounted for by immunoreactive a-MSH. The MSH in the cultured AtT-20 cells and culture medium, which eluted earlier from the Sephadex column, failed to react in the a-MSH radioimmunoassay. The MSH in each extract reacted with antibodies in the P-MSH radioimmunoassay, generating competition curves parallel to each other but less steep than P-MSH standard. Thus, the MSH was immunologically similar, but not identical, to any known P-MSH. The normal mouse pituitary and the AtT-20 tumor cell synthesize two ACTH's, the larger of which may be a precursor molecule, and a smaller MSH molecule which has immunochemical similarities to both a-MSH and P- MSH. {Endocrinology 92: 385, 1973) T HE KNOWN ACTH's and MSH's 5 from several species have important similarities: they are all produced in the adenohypophysis, they are all relatively short polypeptides that share a common sequence of amino acids, and they all have both intrinsic adrenocorticotropic and melanocyte stimulating activities. Previous studies from our laboratory have shown that the concentrations of ACTH and P-MSH, the principal

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confidence: 99%

ACTH and MSH Production by a Single Cloned Mouse Pituitary Tumor Cell Line

et al. 1973

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“…Células neuroendócrinas como as encontradas na hipófise ou na glândula adrenal possuem o aparato necessário para a secreção de hormônios polipeptídeos em resposta a estímulos externos. A maioria dos estudos em células neuroendócrinas tem sido realizada em células derivadas de um tumor de células pituitárias corticotróficas de camundongo, denominada de AtT20 (135,140). Estas células apresentam endoproteases PC2, PC3 (141) assim como glicoquinase (142), porém são deficientes em GLUT2.…”

Section: Engenharia Genética De Células Extra-pancreáticasunclassified

Terapia gênica para o diabetes

Demeterco¹,

Levine²

2001

Arq Bras Endocrinol Metab

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A administração de insulina exógena tem sido a única forma de tratamento disponível para milhões de indivíduos portadores de diabetes mellitus do tipo 1 (insulino-dependente). Embora o transplante de pâncreas tenha sido empregado com sucesso para um número limitado de pacientes, ele ainda é considerado um procedimento invasivo com alto risco de complicações. Por outro lado, estudos preliminares onde o transplante de ilhotas pancreáticas foi realizado sem o emprego de glucocorticóides no esquema de imunossupressão demonstraram resultados extremamente promissores. Entretanto, o emprego de ilhotas pancreáticas, assim como o transplante de pâncreas, enfrenta o problema da escassez de órgãos disponíveis para transplante. Assim, um dos grandes objetivos da terapia gênica para diabetes é a geração de fontes ilimitadas de células que apresentem secreção normal de insulina em resposta ao estímulo da glicose, capazes de serem transplantadas sem a necessidade de imunossupressão sistêmica. Este artigo tem como finalidade revisar como a terapia gênica pode ser empregada na obtenção desta fonte de células, assim como discutir os últimos avanços no campo da biologia celular e molecular em relação ao crescimento e diferenciação da célula beta.

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“…At Z18VIA is a mouse-transplantable pituitary tumor com posed of chromophobe cells which has been found to secrete large amounts of ACTH (5). Its metabolic effects in the LAF1mouse, in which species this tumor will propagate, are essentially those of ACTH.…”

Section: Discussionmentioning

confidence: 99%

“…This tumor is a subline of the ACTHsecreting mouse tumor developed and studied extensively by F u r t h et al (5). LAFX mice in which there was an actively growing node served as donors.…”

Section: Methodsmentioning

confidence: 99%