ACTH(6–9)PGP Peptide Protects SH-SY5Y Cells from H2O2, tert-Butyl Hydroperoxide, and Cyanide Cytotoxicity via Stimulation of Proliferation and Induction of Prosurvival-Related Genes

Abstract

Stabilized melanocortin analog peptide ACTH(6–9)PGP (HFRWPGP) possesses a wide range of neuroprotective activities. However, its mechanism of action remains poorly understood. In this paper, we present a study of the proproliferative and cytoprotective activity of the adrenocorticotropic hormone fragment 6–9 (HFRW) linked with the peptide prolyine–glycyl–proline on the SH-SY5Y cells in the model of oxidative stress-related toxicity. The peptide dose-dependently protected cells from H2O2, tert-butyl hydroperoxide, and KCN and demonstrated proproliferative activity. The mechanism of its action was the modulation of proliferation-related NF-κB genes and stimulation of prosurvival NRF2-gene-related pathway, as well as a decrease in apoptosis.

Structure-based design of promising natural products to inhibit thymidylate kinase from Monkeypox virus and validation using free energy calculations

“…Similarly, SANC00984 which is a Caffeic acid isolated from different species of Salvia family from Africa [ 60 ]. NPC474409 which is 9H-Carbazol-4-Ol isolated from Murraya koenigii possess anti-oxidant activity [ 61 ]. In addition, NPC278434 is an Indole-3-Carboxylic Acid isolated from various plant species with anti-inflammatory activity and demonstrated a docking score of −8.60kcal/mol [ 62 ].…”

NMR Properties of the Cyanide Anion, a Quasisymmetric Two-Faced Hydrogen Bonding Acceptor

“…Hydrogen cyanide and cyanide salts are ubiquitous compounds. They can be both the starting ingredients of life [1,2] and the reason for its end [3][4][5]. The most distinctive feature of the cyanide anion is that its two ends are capable of almost equal non-covalent interactions [6].…”

A Red-Berry Mixture as a Nutraceutical: Detailed Composition and Neuronal Protective Effect

“…Scientific evidence suggests the capacity of polyphenols to reduce the ROS generation in neuronal cellular models [ 36 , 37 , 38 ]. In our study, we used t-BHP treatment to produce mitochondrial injury, which has been widely used to induce oxidative stress in the human neuroblastoma and in differentiated SH-SY5Y cells [ 39 , 40 ]. Multiple assays have been utilized to measure ROS production [ 41 ].…”