ACSL3 is a potential prognostic biomarker for immune infiltration in clear cell renal cell carcinoma

Abstract

ObjectiveLong-chain acyl-coenzyme A synthases (ACSLs) catalyze the activation of fatty acid and are often dysregulated in malignancies. The purpose of this research was to figure out the ACSL3's prognostic value and mechanism in clear cell renal cell carcinoma (ccRCC).MethodsThe expression of ACSL3 in ccRCC was investigated in this work using data from the GEO, TCGA, Oncomine and HPA databases. The expression differences of ACSL3 in the cell lines were further detected by qPCR and Western blot. GEPIA, MethSurv, cBioPortal, and the TIMER were used to perform survival and correlation analysis on ACSL3. GO and KEGG analyses were carried out in R using clusterProfiler and GOplot. Protein-protein interactions (PPI) are constructed from Strings website, and the results were visualized in Cytoscape software.ResultsThe expression level of ACSL3 was significantly reduced in ccRCC tissues, and its mRNA and protein expression were also significantly lower in both renal cancer cell lines. ACSL3 is significantly related to clinical stage, OS, DFS, DNA methylation, and immune-cell infiltration.ConclusionOur findings demonstrated that data mining was capable of eliciting information on ACSL3 levels and its role in genetic regulatory pathways in ccRCC.

Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor Slimming

“…Furthermore, renal cancer cell lines had signifcantly reduced levels of mRNA and protein expression. Additionally, this study demonstrated a strong association between ACSL3 and immune-cell infltration, DNA methylation, and clinical stage [42]. According to Zhang et al's research, it is possible to successfully block the migration, invasion, and proliferation of ccRCC cells by overexpressing ACSL3.…”

Lipids as Targets for Renal Cell Carcinoma Therapy

“…Increased levels of all these enzymes have been suggested to be involved in molecular processes driving cancer growth and progression [ 116 , 117 ]. While a clear view on the relevance of all the ACSL isoforms in RCC is not yet available, it seems that at least ACSL3 could serve as a potential prognostic biomarker for immune infiltration in ccRCC [ 118 ]. Furthermore, ccRCC cells in vitro depend on ACSL3 for lipid droplet formation.…”