ACSL1, CH25H, GPCPD1, and PLA2G12A as the potential lipid-related diagnostic biomarkers of acute myocardial infarction

Abstract

Lipid metabolism plays an essential role in the genesis and progress of acute myocardial infarction (AMI).Herein, we identified and verified latent lipid-related genes involved in AMI by bioinformatic analysis. Lipidrelated differentially expressed genes (DEGs) involved in AMI were identified using the GSE66360 dataset from the Gene Expression Omnibus (GEO) database and R software packages. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted to analyze lipidrelated DEGs. Lipid-related genes were identified by two machine learning techniques: least absolute shrinkage and selection operator (LASSO) regression and support vector machine recursive feature elimination (SVM-RFE). The receiver operating characteristic (ROC) curves were used to descript diagnostic accuracy. Furthermore, blood samples were collected from AMI patients and healthy individuals, and real-time quantitative polymerase chain reaction (RT-qPCR) was used to determine the RNA levels of four lipid-related DEGs. Fifty lipid-related DEGs were identified, 28 upregulated and 22 downregulated. Several enrichment terms related to lipid metabolism were found by GO and KEGG enrichment analyses. After LASSO and SVM-RFE screening, four genes (ACSL1, CH25H, GPCPD1, and PLA2G12A) were identified as potential diagnostic biomarkers for AMI. Moreover, the RT-qPCR analysis indicated that the expression levels of four DEGs in AMI patients and healthy individuals were consistent with bioinformatics analysis results. The validation of clinical samples suggested that 4 lipid-related DEGs are expected to be diagnostic markers for AMI and provide new targets for lipid therapy of AMI.

The Diagnostic Value of ACSL1, ACSL4, and ACSL5 and the Clinical Potential of an ACSL Inhibitor in Non-Small-Cell Lung Cancer

“…An increased expression of ACSL1 has been found in different cancer types, associated with oncogenic functions in liver, colorectal, prostate, breast, and ovarian cancers [ 41 , 42 , 43 , 44 , 45 ]. Additionally, ACSL1 also plays an important role in cardiac and inflammatory diseases [ 46 , 47 ].…”

Identification of mitochondria-related gene biomarkers associated with immune infiltration in acute myocardial infarction

“…demonstrated the high expression of ACSL1, which reduces fatty acid β-oxidation by the peroxisome proliferator-activated receptor-gamma (PPAR-γ) pathway. 19 A cohort study has shown that expression of ACSL1 was significantly increased in the peripheral blood mononuclear cells of patients with AMI and may be a potential molecular biomarker for the early diagnosis of AMI. 20 It is reported that ALDH2 acts as a key mediator of endogenous cytoprotection against myocardial I/R injury, and ALDH2 activation is sufficient to protect the heart from ischemic damage.…”

Ferroptosis and Lipid Metabolism in Acute Myocardial Infarction

“…In premenopausal women, high breast fat accumulation can produce and release inflammatory molecules implicit in vascular damage, atherosclerosis, leading to an increased risk of subsequent cardiovascular adverse events [ 5 ]. Lipid metabolism is of utmost importance in preserving a well-functioning cardiovascular system [ 6 ]. A study showed that metformin may reduce perimeter coronary fat levels by targeting the mediated-glucose cotransporter 2 (SGLT2)/leptin axis, improving adverse cardiovascular outcomes in diabetic patients [ 7 ].…”